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- Clone
- W20066D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B cell activating factor belonging to the TNF family (BAFF), B lymphocytes stimulator (BLyS), TNF- and APOL-related leucocyte expressed ligand 1(TALL1), TNF homolog that activates apoptosis, NKFB, JNK (THANK), TNF13B, CD257
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Recombinant Human BAFF (Cat. No. 559602) (black circles) induces the proliferation of IgM-stimulated primary mouse B cells. Ultra-LEAF™ Purified Anti-Human BAFF (clone W20066D) (purple squares) neutralizes proliferation induced by Recombinant Human BAFF at 6.7 ng/mL in a dose-dependent manner, whereas Ultra-LEAF™ Purified mouse IgG2b, κ isotype control (clone MG2b-57) (Cat. No. 401215) (gray triangles) does not have an effect. The ND50 is 4-24 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 638353 | 100 µg | 344€ | ||||
| 638354 | 1 mg | 908€ | ||||
BAFF is a TNF-α cytokine member (a type II membrane protein) that acts in both a membrane-bound form and soluble cytokine form. The extracellular domain of BAFF shows high homology with APRIL. The soluble form is derived by cleavage in a furin consensus site. In human neutrophils treated with G-CSF, BAFF is processed intracellularly by a furin-type convertase. BAFF acts mainly as a soluble trimer and binds to three receptors with decreasing affinity in the following order: BAFFR > TACI > BCMA. Mice deficient in BAFF or its receptor BAFFR exhibit an obstruction in B cell development after the transitional T1 stage and lack marginal zone (MZ) and conventional B2 B cells. BAFFR-deficient mice and humans (patients with common variable immunodeficiency, or CVID) develop severe B cell lymphopenia due to the arrest of B cell development at the transitional B cell stage. Extreme BAFF production triggers severe autoimmune disorders in mice similar to Systemic Lupus Erythematosus (SLE) and Sjögren’s syndrome. BAFF has been associated with different human diseases such as pancreatic ductal adenocarcinoma (PDAC), autoimmune pancreatitis, SLE, rheumatoid arthritis, Sjögren's syndrome, lymphoid cancers, HIV infection, and B cell malignancies such as non-Hodgkin’s lymphoma (NHL).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human BAFF
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing the proliferation of primary mouse B cells induced by recombinant human BAFF (Cat. Nos. 559602, 559604, 559606, and 559608) at a spike concentration determined from a standard curve. ND50 range: 4 - 24 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details
- Structure
- Trimer
- Distribution
-
Monocytes, macrophages, dendritic cells, neutrophils, and stromal cells of lymphoid organs
- Function
- BAFF induces B cell maturation, proliferation, survival, and immunoglobulin production. BAFF controls the size and composition of mature preimmune B cell pools. BAFF is induced by INF-γ in monocytes.
- Interaction
- Immature B cells, peripheral B-cells, naïve and memory cells
- Ligand/Receptor
- BAFFR, TACI (transmembrane activator and calcium-modulator and cyclophilin ligand interactor), and BCMA (B cell maturation antigen)
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction, Stem Cells
- Molecular Family
- CD Molecules, Cytokines/Chemokines, Growth Factors, Soluble Receptors
- Antigen References
-
- Schneider P, et al. 1999. J Exp Med. 189:1747-56.
- Nardelli B, et al. 2001. Blood. 97:198-204.
- Schiemann B, et al. 2001. Science. 5537:2111-4.
- Scapini P, et al. 2003. J Exp Med. 197:297-302.
- Yeramilli VA and Knight KL. 2010. J Immunol. 184:5527-36.
- Scholz JL and Cancro MP. 2012. Immunol. Lett. 143:2-8.
- Sindhava VJ, et al. 2013. Front Immunol. 4:37.
- Koizumi M, et al. 2013. PLoS One. 8:e71367.
- Gene ID
- 10673 View all products for this Gene ID
- UniProt
- View information about BAFF on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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