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Alexa Fluor® 700 anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_Alx700_022008
Human peripheral blood lymphocytes stained with HIB19 Alexa Fluor® 700
  • HIB19_Alx700_022008
    Human peripheral blood lymphocytes stained with HIB19 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
302225 25 µg 101€
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302226 100 µg 215€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Jagannathan P, et al. 2017. Sci Rep. 10.1038/s41598-017-10624-3. PubMed
  2. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  3. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  4. Berry MR et al. 2017. Cell. 170(5):860-874 . PubMed
  5. Fasbender F, et al. 2017. Front Immunol. 0.88125. PubMed
  6. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  7. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  8. Plum T, et al. 2020. Immunity. 52(2):404-416. PubMed
  9. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  10. Boyle M, et al. 2015. J Infect Dis. 212: 416-425. PubMed
  11. Quast I, et al. 2015. Neurol Neuroimmunol Neuroinflamm. 2: e148. PubMed
  12. Inglis H, et al. 2015. J Vis Exp. 97: 52484. PubMed
  13. Miles B, et al. 2015. Nat Commun. 6: 8608. PubMed
  14. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  15. Brouwer PJM, et al. 2020. Science. 369:643. PubMed
  16. Wang J, et al. 2020. Cell. 183:1264. PubMed
  17. Hood SP, et al. 2020. Elife. 9:00. PubMed
  18. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  19. Rubio-Pérez C, et al. 2021. Nat Commun. 1503:12. PubMed
  20. Shin CH, et al. 2020. J Immunol. 205:3179. PubMed
  21. Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
  22. Feldman J, et al. 2021. Sci Immunol. 6:eabl5842. PubMed
  23. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  24. Harris LD, et al. 2020. Front Cell Infect Microbiol. 10:120. PubMed
  25. Shin JJ, et al. 2022. J Clin Immunol. :. PubMed
  26. Port JR, et al. 2020. J Virol. 94:. PubMed
  27. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  28. Faissner S, et al. 2022. Front Immunol. 13:980526. PubMed
  29. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
  30. Wildner NH, et al. 2021. J Leukoc Biol. 109:77. PubMed
  31. Sefik E, et al. 2022. Nature. 606:585. PubMed
  32. Du Y, et al. 2022. J Exp Med. 219: . PubMed
RRID
AB_493750 (BioLegend Cat. No. 302225)
AB_493751 (BioLegend Cat. No. 302226)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 3    Revision Date: 06.27.2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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