Loading...

Alexa Fluor® 700 anti-human CD45 Antibody

Pricing & Availability
Clone
HI30 (See other available formats)
Regulatory Status
RUO
Workshop
IV N816
Other Names
LCA, T200
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
HI30_Alx700_111407
Human peripheral blood lymphocytes stained with HI30 Alexa Fluor® 700
  • HI30_Alx700_111407
    Human peripheral blood lymphocytes stained with HI30 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
304023 25 µg 100€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
304024 100 µg 212€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. BÖiers C, et al. 2018. Dev Cell. 44:362. PubMed
  2. Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed
  3. Garfall AL, et al. 2018. JCI Insight. 3. PubMed
  4. Zheng S, et al. 2018. Mol Syst Biol. 14:e8041. PubMed
  5. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  6. Kinchen J et al. 2018. Cell. 175(2):372-386 . PubMed
  7. Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed
  8. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  9. Marquardt N, et al. 2019. Nat Commun. 10:3841. PubMed
  10. Ludwig LS et al. 2019. Cell. 176(6):1325-1339 . PubMed
  11. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  12. Neal JT, et al. 2018. Cell. 175:1972. PubMed
  13. Perez–Zsolt D, et al. 2019. Front Immunol. 0.989583333. PubMed
  14. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  15. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  16. Fedyk ER, et al. 2020. British Journal of Clinical Pharmacology. 86(7):1314-1325.. PubMed
  17. Butch ER, et al. 2020. Cancer Research. 79(12):3112-3124.. PubMed
  18. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  19. Wu X, et al. 2020. Front Immunol. 1.922222222. PubMed
  20. Wu Y, et al. 2013. J Biosci Bioeng. 64:169. PubMed
  21. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  22. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  23. Marquardt N, et al. 2015. J Immunol. 194:2467. PubMed
  24. Björklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed
  25. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  26. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  27. Ivarsson M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.50. PubMed
  28. Nilsson A, et al. 2016. PLoS One. 11: 0158369. PubMed
  29. Cartellieri M, et al. 2016. Blood Cancer J. 6:e458. PubMed
  30. Marquardt N, et al. 2016. J Immunol. 197: 3069 - 3075. PubMed
  31. Qiu J, et al. 2020. Cell Rep. 33:108465. PubMed
  32. Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed
  33. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  34. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  35. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  36. Williams DW, et al. 2021. Cell. . PubMed
  37. Louka E, et al. 2021. Journal of Experimental Medicine. 218(2):. PubMed
  38. Hernndez DC, et al. 2021. Immunity. :. PubMed
  39. Fontana D, et al. 2020. Hemasphere. e497:4. PubMed
  40. Calabrese DR, et al. 2020. J Clin Invest. . PubMed
  41. Jühling F, et al. 2021. Life Sci Alliance. 4: . PubMed
  42. Poon MML, et al. 2021. Cell Rep. 37:110071. PubMed
  43. Takano T, et al. 2022. Cell Rep Med. 3:100631. PubMed
  44. Bessy T, et al. 2021. J Cell Biol. 220:. PubMed
  45. Strand K, et al. 2022. Front Endocrinol (Lausanne). 13:856530. PubMed
  46. Sun K, et al. 2022. Nat Commun. 13:4943. PubMed
  47. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  48. Li L, et al. 2020. J Transl Med. 18:363. PubMed
  49. Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
  50. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  51. Khatamzas E, et al. 2022. Nat Commun. 13:5586. PubMed
  52. Shen Z, et al. 2022. Immun Ageing. 19:55. PubMed
  53. Pal R, et al. 2020. Curr Protoc Cytom. 92:e69. PubMed
  54. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  55. Mashayekhi M, et al. 2022. Prostaglandins Other Lipid Mediat. 158:106604. PubMed
  56. Filipello F, et al. 2023. Acta Neuropathol. 145:749. PubMed
  57. Wang X, et al. 2023. Stem Cell Rev Rep. . PubMed
RRID
AB_493760 (BioLegend Cat. No. 304023)
AB_493761 (BioLegend Cat. No. 304024)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org
Go To Top Version: 3    Revision Date: 06.27.2014

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account