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Alexa Fluor® 700 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_Alx700_080408.jpg
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Alexa Fluor® 700 (filled histogram) or rat IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
  • 6D5_Alx700_080408.jpg
    C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Alexa Fluor® 700 (filled histogram) or rat IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
115527 25 µg 76€
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115528 100 µg 172€
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  2. Kenderes KJ, et al. 2018. Cell Rep. 24:824. PubMed
  3. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  4. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  5. Bennion BG, et al. 2019. J Virol. 93. PubMed
  6. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  7. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  8. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  9. Kwong Chung CK, et al. 2017. Immunology. 150:221. PubMed
  10. Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed
  11. Chitu V, et al. 2020. Cell Reports. 30(9):3004-3019. PubMed
  12. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  13. Li J, et al. 2010. J Immunol. 184:6142. PubMed
  14. Perry D, et al. 2012. J Immunol. 189:793. PubMed
  15. Holm J, et al. 2015. PLoS One. 10:125495. PubMed
  16. Cruz F, et al. 2016. Stem Cells Trans Med. 5: 488-499. PubMed
  17. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  18. Gorman M, et al. 2016. J Virol. 90: 8212 - 8225. PubMed
  19. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  20. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  21. Aldrich AL, et al. 2021. The Journal of Immunology. 206(4):751-765. PubMed
  22. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  23. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  24. Yewdell WT, et al. 2020. Immunity. 53(5):952-970.e11. PubMed
  25. Doron I, et al. 2021. Cell. 184(4):1017-1031.e14. PubMed
  26. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  27. Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
  28. Giampazolias E, et al. 2021. Cell. . PubMed
  29. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  30. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  31. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  32. Vogel AB, et al. 2021. Nature. 592:283. PubMed
  33. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  34. Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
  35. Li H, et al. 2020. Nature. 584:274. PubMed
  36. Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed
  37. Vijay R, et al. 2021. J Exp Med. 218:. PubMed
  38. Boldison J, et al. 2020. Cell Mol Immunol. 17:843. PubMed
  39. Roy R, et al. 2022. Elife. 11:. PubMed
  40. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  41. Rinaldi S, et al. 2021. PLoS Pathog. 17:e1009533. PubMed
  42. Andreas N, et al. 2021. Arthritis Res Ther. 23:222. PubMed
  43. Wu S, et al. 2022. BMC Immunol. 23:17. PubMed
  44. Kitagawa S, et al. 2022. NPJ Vaccines. 7:115. PubMed
  45. Matsuda T, et al. 2022. iScience. 25:105324. PubMed
  46. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  47. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  48. Orvain C, et al. 2022. Arthritis Res Ther. 24:13. PubMed
  49. du Halgouet A, et al. 2023. Immunity. 56:78. PubMed
  50. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  51. Patton T, et al. 2023. Cell Death Dis. 14:111. PubMed
  52. Xiao R, et al. 2022. J Immunol. 208:2109. PubMed
RRID
AB_493734 (BioLegend Cat. No. 115527)
AB_493735 (BioLegend Cat. No. 115528)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 3    Revision Date: 06.27.2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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