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APC anti-human CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A019D5 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Mouse IgG1, κ
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Product Citations
publications
A019D5_APC_CD127_Antibody_FC_1_102512
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) APC (top) or mouse IgG1 APC isotype control (bottom).
  • A019D5_APC_CD127_Antibody_FC_1_102512
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) APC (top) or mouse IgG1 APC isotype control (bottom).
  • A019D5_APC_CD127_Antibody_FC_2_102512
See APC spectral data
Cat # Size Price Quantity Check Availability Save
351315 25 tests 114€
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351342 100 µg 237€
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351316 100 tests 252€
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD127
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for the relevant formats) application: proteogenomics1.

Application References

(PubMed link indicates BioLegend citation)
  1. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  2. Wang L, et al. 2018. Oncol Lett. 15:8635. PubMed
  3. Kim SY, et al. 2018. Invest Ophthalmol Vis Sci. 59:5693. PubMed
  4. Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed
  5. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  6. Li J, et al. 2017. Toxicology. 378:17. PubMed
  7. Rochman Y, et al. 2015. PLoS One. 10:122198. PubMed
  8. Christophersen A, et al. 2016. J Immunol. 196: 2819 - 2826. PubMed
  9. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  10. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  11. Imura Y, et al. 2020. JCI Insight. :5. PubMed
  12. Goods BA, et al. 2021. JCI Insight. 6: . PubMed
  13. Vandooren J, et al. 2021. Front Immunol. 12:701739. PubMed
  14. Shen C, et al. 2021. Front Immunol. 12:680055. PubMed
  15. Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed
  16. Gamradt S, et al. 2021. iScience. 24:103312. PubMed
  17. Hixon JA, et al. 2020. Leukemia. 34:35. PubMed
  18. Grigoriou M, et al. 2021. Cancer Immunol Res. 9:726. PubMed
  19. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  20. Sehgal R, et al. 2022. Front Immunol. 13:828949. PubMed
  21. Nabatanzi R, et al. 2021. BMC Immunol. 22:59. PubMed
  22. Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed
  23. Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed
  24. Batikan O, et al. 2022. J Coll Physicians Surg Pak. 32:1004. PubMed
  25. Grivas A, et al. 2022. Front Immunol. 13:964274. PubMed
  26. Sudmeier LJ, et al. 2022. Cell Rep Med. 3:100620. PubMed
  27. Cui B, et al. 2023. Int Immunopharmacol. 114:109541. PubMed
  28. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  29. Avital G, et al. 2022. Cell Rep. 41:111477. PubMed
RRID
AB_10900814 (BioLegend Cat. No. 351315)
AB_2564137 (BioLegend Cat. No. 351342)
AB_10900804 (BioLegend Cat. No. 351316)

Antigen Details

Structure
Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.

Gene ID
3575 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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