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APC anti-human HLA-DR Antibody

Pricing & Availability
Clone
L243 (See other available formats)
Regulatory Status
RUO
Other Names
Major Histocompatibility Class II, MHC class II
Isotype
Mouse IgG2a, κ
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Product Citations
publications
L243_APC_072607
Human peripheral blood lymphocytes stained with L243 APC
  • L243_APC_072607
    Human peripheral blood lymphocytes stained with L243 APC
See APC spectral data
Cat # Size Price Quantity Check Availability Save
307609 25 tests 67€
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307610 100 tests 155€
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Description

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19

Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).

Application References

(PubMed link indicates BioLegend citation)
  1. Brodsky F. 1984. Immunogenetics 19:179.
  2. Robbins P, et al. 1987. Human Immunol. 18:301.
  3. Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161.
  4. Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC)
  5. Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC)
  6. Zipf T, et al. 1981. Cancer Res. 41:4786.
  7. Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion)
  8. Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB)
  9. Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block)
  10. Wang RF, et al. 1999. Science 284:1351. (Block)
  11. Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed
  12. Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed
  13. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  14. Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed
  15. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  16. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  17. Stein R, et al. 2011. Leuk. Lymphoma 52:273.
  18. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  19. Moro M, et al. 2005. BMC Immunol. 6:24.
  20. Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
  21. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  22. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Eitas T, et al. 2017. PLoS One. 10.1371/journal.pone.0184164. PubMed
  2. Yang M, et al. 2018. Oncol Lett. 15:3918. PubMed
  3. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  4. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  5. Roy Chowdhury R, et al. 2018. Nature. 560:644. PubMed
  6. Jin Y et al. 2019. Nature communications. 10(1):391 . PubMed
  7. Singh N, et al. 2017. J Endocrinol. 235:69. PubMed
  8. Wang Y, et al. 2019. J Transl Med. 17:93. PubMed
  9. Sasano T, et al. 2018. Clin Cancer Res. 24:4018. PubMed
  10. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  11. Vielle NJ, et al. 2018. Sci Rep. 8:5440. PubMed
  12. Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
  13. Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
  14. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  15. Zizzo G, et al. 2012. J Immunol. 189:3508. PubMed
  16. Li Z, et al. 2014. PLoS One. 9:106064. PubMed
  17. Li L, et al. 2015. J Am Soc Nephrol. 26: 2183-2197. PubMed
  18. Pate K, et al. 2015. J Infect Dis. 212: 1387 - 1396. PubMed
  19. Bsat M, et al. 2015. J Leukoc Biol. 98: 671 - 681. PubMed
  20. Scottà C, et al. 2016. Haematologica. 101: 91 - 100. PubMed
  21. Kotsiou E, et al. 2016. Blood. 128: 72 - 81. PubMed
  22. Alfaro C, et al. 2016. Clin Cancer Res. 22: 3924 - 3936. PubMed
  23. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  24. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  25. M?czy?ska J, et al. 2020. Cell Death Dis. 1.073611111. PubMed
  26. Nazari-Shafti TZ, et al. 2020. Biomolecules. 10:00. PubMed
  27. Wenthe J, et al. 2021. Cancer Immunology Immunotherapy. . PubMed
  28. Cai J, et al. 2021. eLife. 10:00. PubMed
  29. Ramaswamy A, et al. 2021. Immunity. 54(5):1083-1095.e7. PubMed
  30. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
  31. Gorby C, et al. 2020. Sci Signal. :13. PubMed
  32. Garcia EG, et al. 2020. Leukemia. 35:679. PubMed
  33. Parenti S, et al. 2021. NPJ Precis Oncol. 5:4. PubMed
  34. Seery V, et al. 2021. EBioMedicine. 67:103357. PubMed
  35. Cho JH, et al. 2021. Nat Commun. 12:792. PubMed
  36. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  37. Wu H, et al. 2022. Biomolecules. 12:. PubMed
  38. Liu Y, et al. 2022. iScience. 25:104405. PubMed
  39. Porter RL, et al. 2022. J Clin Invest. :. PubMed
  40. Han J, et al. 2022. FEBS J. 289:417. PubMed
  41. Xu L, et al. 2022. Int J Mol Sci. 23:. PubMed
  42. Diamantopoulos PT, et al. 2022. Cancers (Basel). 14:. PubMed
  43. Perera MR, et al. 2022. Int J Mol Sci. 23:. PubMed
  44. Murphy DM, et al. 2022. J Clin Invest. Online ahead of print. PubMed
  45. Johnson RK, et al. 2022. Sci Rep. 12:19920. PubMed
  46. El Kharbili M, et al. 2022. FASEB J. 36:e22300. PubMed
  47. Pioch J, et al. 2022. J Immunol Methods. 507:113308. PubMed
  48. Parasar P, et al. 2022. Am J Reprod Immunol. 88:e13614. PubMed
  49. Li H, et al. 2023. Clin Rheumatol. 42:1327. PubMed
  50. Yang Y, et al. 2023. Cancers (Basel). 15:. PubMed
RRID
AB_314687 (BioLegend Cat. No. 307609)
AB_314688 (BioLegend Cat. No. 307610)

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Peptide presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138.
3. Edwards JA, et al. 1986. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID
UniProt
View information about HLA-DR on UniProt.org
Go To Top Version: 2    Revision Date: 06.17.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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