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APC anti-mouse/human IL-5 Antibody

Pricing & Availability
Clone
TRFK5 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T-cell replacing factor-1 (TRF-1)
Isotype
Rat IgG1, κ
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Product Citations
publications
TRFK5_APC_IL-5_Antibody_101322
Th2 polarized BALB/c splenocytes were stimulated with PMA + ionomycin for 6 hours and then were stained with anti-mouse CD3 PE, and intracellularly stained with anti-mouse IL-5 (clone TRFK5) APC (left) or rat IgG1 APC isotype control (right).
  • TRFK5_APC_IL-5_Antibody_101322
    Th2 polarized BALB/c splenocytes were stimulated with PMA + ionomycin for 6 hours and then were stained with anti-mouse CD3 PE, and intracellularly stained with anti-mouse IL-5 (clone TRFK5) APC (left) or rat IgG1 APC isotype control (right).
See APC spectral data
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504305 25 µg 82€
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504306 100 µg 263€
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Description

IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse,Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partially-purified T cell clone supernatant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA1,3,4 or ELISPOT2,6 Capture: The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Flow Cytometry: The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations.

Neutralization1,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318).

Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections7, and immunocytochemistry.

Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  2. Klinman, D., et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.
  3. Swain, S., et al. 1990. J. Immunol. 145:3796.
  4. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  5. Assenmacher, M., et al. 1994. Eur. J. Immunol. 24:1097.
  6. Karulin, A., et al. 2000. J. Immunol. 164:1862.
  7. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Fan, W. Y., et al. 2001. Exp. Biol. Med. 226:1045.
  9. Sitkauskiene, B., et al. 2005. Respir Res. 6:33.
Product Citations
  1. Sun X, et al. 2017. Microbes Infect. 10.1016/j.micinf.2017.08.007. PubMed
  2. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  3. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  4. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  5. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  6. Shah RR, et al. 2019. Sci Rep. 9:11520. PubMed
  7. Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed
  8. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  9. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  10. Singh A, et al. 2020. Cell Rep. 32:108153. PubMed
  11. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  12. Williams J, et al. 2013. Am J Physiol Lung Cell Mol Physiol. 305:693. PubMed
  13. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  14. Colliou N, et al. 2015. Toxins. 7: 3805 - 3817. PubMed
  15. Schulze B, et al. 2016. Pathog Dis. 74: ftw020. PubMed
  16. Chen Z, et al. 2016. Nat Commun. 7:11596. PubMed
  17. Schütze N, et al. 2016. J Immunol. 197: 2653 - 2664. PubMed
  18. Corbett KS, et al. 2020. Nature. 586:567. PubMed
  19. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  20. DiPiazza AT, et al. 2021. Immunity. 54(8):1869-1882.e6. PubMed
  21. Wei H, et al. 2021. Malar J. 20:89. PubMed
  22. Baldwin SL, et al. 2021. PLoS One. 16:e0247990. PubMed
  23. Gu C, et al. 2021. Front Immunol. 12:678036. PubMed
  24. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  25. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  26. Peng L, et al. 2022. Cell Rep Med. 3:100634. PubMed
  27. Yu KK, et al. 2021. JCI Insight. 6:. PubMed
  28. Boothby IC, et al. 2021. Nature. 599:667. PubMed
  29. Nakajima T, et al. 2021. J Exp Med. 218:. PubMed
  30. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  31. Ai L, et al. 2023. Cell Discov. 9:9. PubMed
  32. Nanishi E, et al. 2023. NPJ Vaccines. 8:18. PubMed
  33. Vesin B, et al. 2022. Mol Ther. 30:2984. PubMed
  34. Jowett GM, et al. 2022. Cell Rep. 40:111281. PubMed
  35. Corbière V, et al. 2023. Front Immunol. 14:1101366. PubMed
RRID
AB_315329 (BioLegend Cat. No. 504305)
AB_315330 (BioLegend Cat. No. 504306)

Antigen Details

Structure
Cytokine; dimer; 40-50 kD (Mammalian)
Bioactivity
Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources
Mast cells, T cells, eosinophils
Cell Targets
Eosinophils, B cells
Receptors
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immunol. 35:593.

Gene ID
16191 View all products for this Gene ID 3567 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org
Go To Top Version: 2    Revision Date: 10.13.2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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