Brilliant Violet 421™ anti-human TCR α/β Antibody

Product Details

Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone.  Other anti-CD3 clones may compete out the binding of IP26.  

Application References

(PubMed link indicates BioLegend citation)

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. (FC)
2. Joseph A, et al. 2008. J. Virol. 82:3078. (FC) PubMed
3. Pinto JP, et al. 2010. Immunology. 130:217. PubMed

Product Citations

1. Goel G, et al. 2019. Sci Adv. 5:eaaw7756. PubMed
2. Mayassi T, et al. 2019. Cell. 176:967. PubMed
3. Schreurs RRCE, et al. 2019. Immunity. 50:462. PubMed
4. Barbarin A, et al. 2017. Front Immunol. 8:316. PubMed
5. Schreurs RRCE, et al. 2021. Mucosal Immunology. 14(3):605-614. PubMed
6. Hamilton JR, et al. 2021. Cell Reports. 35(9):109207. PubMed
7. Cho JH, et al. 2021. Nat Commun. 12:792. PubMed
8. Möller L, et al. 2022. Nat Commun. 13:4550. PubMed
9. Lee SA, et al. 2020. J Immunol. 204:586. PubMed
10. Shaw DG, et al. 2023. Nat Med. 29:1520. PubMed

RRID

AB_2562077 (BioLegend Cat. No. 306721)
AB_2562805 (BioLegend Cat. No. 306722)

Antigen Details

Structure

Ig superfamily, with CD3 forms CD3/TCR complex

Distribution

T cells, thymocytes

Function

Antigen recognition, T cell activation

Ligand/Receptor

Peptide bound to MHC

Cell Type

T cells, Thymocytes, Tregs

Biology Area

Adaptive Immunity, Immunology

Molecular Family

TCRs

Antigen References

1. Marchalonis J, et al. 2002. J. Mol. Recognit. 15:260.

Gene ID

6955 View all products for this Gene ID 6957 View all products for this Gene ID

UniProt

View information about TCR alpha/beta on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis