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Brilliant Violet 605™ anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136_BV605_NK-1.1_Antibody_FC_1_071013
C57BL/6 mouse splenocytes were stained with CD49b/DX5 APC and NK1.1 (clone PK136) Brilliant Violet 605™ (top) or mouse IgG2a, κ Brilliant Violet 605™ isotype control (bottom).
  • PK136_BV605_NK-1.1_Antibody_FC_1_071013
    C57BL/6 mouse splenocytes were stained with CD49b/DX5 APC and NK1.1 (clone PK136) Brilliant Violet 605™ (top) or mouse IgG2a, κ Brilliant Violet 605™ isotype control (bottom).
  • PK136_BV605_NK-1.1_Antibody_FC_2_071013
See Brilliant Violet 605™ spectral data
Cat # Size Price Quantity Check Availability Save
108739 125 µL 172€
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108753 50 µg 227€
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108740 500 µL 352€
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  2. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  3. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  4. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  5. Wong E, et al. 2019. Cell Rep. 29:3047. PubMed
  6. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  7. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  8. Cunningham C, et al. 2016. PLoS Pathog. 12: 1005356. PubMed
  9. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  10. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  11. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  12. Barberio AE, et al. 2020. ACS Nano. 14:11238. PubMed
  13. Takushi SE, et al. 2020. Hum Gene Ther. 31:626. PubMed
  14. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  15. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  16. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  17. Hobbs BE, et al. 2021. Pathogens. 10:. PubMed
  18. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  19. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
  20. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  21. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  22. Wang J, et al. 2022. Clin Transl Med. 12:e718. PubMed
  23. Boddupalli CS, et al. 2022. Elife. 11:. PubMed
  24. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
  25. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  26. Rodrigues PB, et al. 2022. Cells. 11: . PubMed
  27. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  28. Jowett GM, et al. 2022. Cell Rep. 40:111281. PubMed
  29. Barberio AE, et al. 2023. Bioeng Transl Med. 8:e10453. PubMed
RRID
AB_2562273 (BioLegend Cat. No. 108739)
AB_2686977 (BioLegend Cat. No. 108753)
AB_2562274 (BioLegend Cat. No. 108740)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Related FAQs

There are no FAQs for this product.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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