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FITC anti-mouse CD80 Antibody

Pricing & Availability
Clone
16-10A1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, Ly-53
Isotype
Armenian Hamster IgG
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Product Citations
publications
16-10A1
LPS-stimulated (3 days) BALB/c splenocytes stained with 16-10A1 FITC
  • 16-10A1
    LPS-stimulated (3 days) BALB/c splenocytes stained with 16-10A1 FITC
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
104705 50 µg 57€
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104706 500 µg 164€
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Description

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  2. Nechama M, et al. 2018. Nat Commun. 9:1603. PubMed
  3. Porciello N, et al. 2018. Nat Commun. 9:1080. PubMed
  4. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  5. Jiang H, et al. 2018. Front Pharmacol. 9:612. PubMed
  6. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  7. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  8. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  9. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  10. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  11. Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed
  12. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  13. Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed
  14. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  15. Ji W, et al. 2018. Evid Based Complement Alternat Med. 2017:3272649. PubMed
  16. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  17. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
  18. Bode K, et al. 2020. Cell Reports. 29(13):4435-4446.e9.. PubMed
  19. Li X, et al. 2009. PLoS One. 4:e6385. PubMed
  20. Kis-Toth K, et al. 2011. J Immunol. 187:1273. PubMed
  21. Gravano D, et al. 2011. PLoS One. 6:e13517. PubMed
  22. Benhamron S, et al. 2012. PLoS One. 7:e35602. PubMed
  23. Liang Y, et al. 2014. J Immunol. 192:1277. PubMed
  24. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  25. Tassi I, et al. 2014. J Immunol. 193:4303. PubMed
  26. Li X, et al. 2014. PLoS One. 9:108192. PubMed
  27. Balashov L 2015. Mult Scler. 21: 1131-1138. PubMed
  28. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  29. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  30. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  31. Sadiq BA, et al. 2020. Current Protocols in Immunology. 131(1):e115. PubMed
  32. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  33. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  34. Pandey V, et al. 2021. eLife. 0.416666666666667. PubMed
  35. Zheng B, et al. 2021. Bioact Mater. 6:3879. PubMed
  36. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  37. Saito S, et al. 2020. Nutrients. 12:. PubMed
  38. Jiang Y, et al. 2021. Nat Commun. 12:742. PubMed
  39. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  40. Shi GN, et al. 2021. Ther Adv Med Oncol. 13:1758835920987056. PubMed
  41. Kornstädt L, et al. 2021. Front Immunol. 11:607048. PubMed
  42. Wang G, et al. 2021. Nat Commun. 12:5733. PubMed
  43. Wang L, et al. 2021. Sci Adv. 7:eabj4796. PubMed
  44. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  45. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  46. Ren S, et al. 2022. Int J Biol Sci. 18:166. PubMed
  47. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  48. Jiang Q, et al. 2022. Theranostics. 12:59. PubMed
  49. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  50. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  51. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  52. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  53. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  54. Zhou Y, et al. 2020. Front Pharmacol. 10:1579. PubMed
  55. Bommireddy R, et al. 2020. Vaccines (Basel). 8:. PubMed
  56. Kawai H, et al. 2021. Asian Pac J Allergy Immunol. :. PubMed
  57. Matsumura F, et al. 2021. J Immunol. 207:938. PubMed
  58. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  59. Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
  60. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
  61. Gómez LA, et al. 2020. Front Microbiol. 11:1586. PubMed
  62. Blank A, et al. 2021. J Pathol. 253:160. PubMed
  63. Funk KE, et al. 2021. Aging Cell. 20:e13412. PubMed
  64. Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
  65. Xiong W, et al. 2022. Adv Sci (Weinh). 9:e2103029. PubMed
  66. Liang F, et al. 2021. RSC Adv. 11:35331. PubMed
  67. Chen C, et al. 2022. Sci Adv. 8:eabo4363. PubMed
  68. Chen Y, et al. 2023. Adv Healthc Mater. 12:e2202085. PubMed
  69. Wang H, et al. 2022. J Nanobiotechnology. 20:513. PubMed
  70. Kimura Y, et al. 2023. Front Immunol. 14:1070196. PubMed
  71. Vesin B, et al. 2022. Mol Ther. 30:2984. PubMed
  72. Akhtar MN, et al. 2022. MBio. 13:e0330921. PubMed
  73. Zhuang WR, et al. 2023. Nat Commun. 14:1675. PubMed
  74. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  75. Fu S, et al. 2023. Nat Commun. 14:2248. PubMed
  76. Brigo N, et al. 2023. Cells. 12:. PubMed
  77. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  78. Wang D, et al. 2023. Nat Commun. 14:2943. PubMed
RRID
AB_313126 (BioLegend Cat. No. 104705)
AB_313127 (BioLegend Cat. No. 104706)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org
Go To Top Version: 2    Revision Date: 02.18.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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