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PE anti-human CD123 Antibody

Pricing & Availability
Clone
6H6 (See other available formats)
Regulatory Status
RUO
Other Names
IL-3Rα, IL-3 Receptor alpha
Isotype
Mouse IgG1, κ
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Product Citations
publications
6H6
Human peripheral blood lymphocytes stained with 6H6 PE and CD45RO PE/Cy5
  • 6H6
    Human peripheral blood lymphocytes stained with 6H6 PE and CD45RO PE/Cy5
See PE spectral data
Cat # Size Price Quantity Check Availability Save
306005 25 tests 66€
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306006 100 tests 140€
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Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IL-3Rα transfected COS cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.

Application References

(PubMed link indicates BioLegend citation)
  1. Sun Q, et al. 1996. Blood 87:83. (IP, WB)
  2. Herling M, et al. 2003. Blood 101:5007. (IHC)
  3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  4. Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
  5. Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
  6. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
  7. Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
Product Citations
  1. Zaro BW, et al. 2019. J Immunol. 202:2737. PubMed
  2. Felce JH, et al. 2018. Sci Signal. 11:eaat0756. PubMed
  3. Luchsinger LL, et al. 2020. Cell Stem Cell. 25(2):225-240. PubMed
  4. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
  5. Han L, et al. 2012. PLoS One. 4:e7989. PubMed
  6. Randrianarison-Huetz V, et al. 2010. Blood. 115:2784. PubMed
  7. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  8. Woo MS, et al. 2021. iScience. 24(7):102752. PubMed
  9. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  10. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
  11. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  12. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  13. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  14. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  15. Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
  16. Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
  17. Sefik E, et al. 2022. Nature. 606:585. PubMed
  18. Shahid AM, et al. 2022. PLoS One. 17:e0278209. PubMed
  19. Baudet A, et al. 2022. Bio Protoc. 12:e4353. PubMed
  20. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
RRID
AB_314579 (BioLegend Cat. No. 306005)
AB_314580 (BioLegend Cat. No. 306006)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution

Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes

Function
Hematopoietic cell proliferation, differentiation
Ligand/Receptor
IL-3
Cell Type
Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Miyajima A, et al. 1993. Blood 82:1960.

Gene ID
3563 View all products for this Gene ID
UniProt
View information about CD123 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04.18.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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