- Clone
- JS11 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI N-L060
- Other Names
- DAF (Decay Accelerating Factor)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![JS11 JS11](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/JS11.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes stained with JS11 PE, then detected with anti-mouse IgG FITC -
Pre-lysed human blood leukocytes were stained with CD55 (clone JS11) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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311308 | 100 tests | 215€ |
CD55 is a 60-70 kD glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein also known as decay-accelerating factor (DAF). It is expressed on hematopoietic cells including erythrocytes and many non-hematopoietic cells. CD55 accelerates the dissociation of the components of the C3-convertases (namely C2a from C4b in the C4bC2a complex, a C3-convertase of the classical pathway, and factor Bb from the C3bBb complex, a C3-convertase of the alternative pathway) to protect cells from inappropriate damage caused by autologous complement. CD55 has been reported to reduce the efficiency of NK cell lysis and induce signal transduction in T cells. CD55 has also been shown to interact with CD97 and bind to Coxsackie and Echovirus.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Peyron P, et al. 2000. J. Immunol. 165:5186
- Product Citations
- RRID
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AB_314865 (BioLegend Cat. No. 311308)
Antigen Details
- Structure
- GPI-achored regulator of complement activation (RCA), type I membrane glycoprotein, 60-70 kD
- Distribution
-
Hematopoietic cells including erythrocytes and many non-hematopoietic cells
- Function
- Protects against inappropriate complement activation and cell damage, induces signal transduction, reduces the efficiency of NK cell lysis
- Ligand/Receptor
- C3b, C4b, CD97, Coxsackie viruses
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
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1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Hamann J, et al. 1996. J. Exp. Med. 184:1185.
3. Fujita T, et al. 1987. J. Exp. Med. 166:1221 - Gene ID
- 1604 View all products for this Gene ID
- UniProt
- View information about CD55 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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