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PE anti-human CD69 Antibody

Pricing & Availability
Clone
FN50 (See other available formats)
Regulatory Status
RUO
Workshop
IV A91
Other Names
Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Isotype
Mouse IgG1, κ
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Product Citations
publications
FN50
PMA + ionomycin stimulated (6 hours) human lymphocytes stained with FN50 PE
  • FN50
    PMA + ionomycin stimulated (6 hours) human lymphocytes stained with FN50 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
310905 25 tests 53€
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310906 100 tests 115€
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Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD69 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
  3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
  4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
  5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
  6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  2. Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
  3. Zimmermann M, et al. 2019. Front Immunol. 10:829. PubMed
  4. Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
  5. Halkias J, et al. 2019. J Clin Invest. 130:3562. PubMed
  6. Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
  7. Wallstabe L et al. 2019. JCI Insight. 4(18) pii: 126345. PubMed
  8. Shan L, et al. 2017. Immunity. 47:766. PubMed
  9. Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed
  10. Guenther C, et al. 2019. Front Immunol. 10:1138. PubMed
  11. Stuart T, et al. 2019. Cell. 177:1888. PubMed
  12. Li W, et al. 2020. Immunity. 53(2):456-470. PubMed
  13. Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed
  14. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  15. Fiaux PC, et al. 2020. PLoS Comput Biol. 16:e1008194. PubMed
  16. Thakral D, et al. 2008. J Immunol. 180:7431. PubMed
  17. Costantini C, et al. 2010. Int Immunol. 1.490972222. PubMed
  18. Jaudszus A, et al. 2013. J Lipid Res. 54:923. PubMed
  19. Park S, et al. 2015. J Biol Chem. 290: 17349 - 17366. PubMed
  20. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  21. Su S, et al. 2016. Sci Rep. 6:20070. PubMed
  22. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  23. Wang R, et al. 2016. Proc Natl Acad Sci U S A. 113: 11501 - 11506. PubMed
  24. Zhou Y, et al. 2016. J Leukoc Biol. 100: 1201 - 1211. PubMed
  25. Kieffer T, et al. 2017. J Reprod Immunol. 10.1016/j.jri.2016.11.004. PubMed
  26. Oikawa D, et al. 2020. Front Immunol. 11:601926. PubMed
  27. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
  28. Cohen Z, et al. 2020. Biomed Rep. 13:17. PubMed
  29. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  30. Hoseini SS, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
  31. Luoma AM, et al. 2020. Cell. 182(3):655-671.e22. PubMed
  32. Ng KW, et al. 2019. eLife. 0.333333333333333. PubMed
  33. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  34. Lucas C, et al. 2021. Nature. Online ahead of print. PubMed
  35. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
  36. Kongsomboonvech AK, et al. 2020. PLoS Pathog. 16:e1008327. PubMed
  37. Arvindam US, et al. 2021. Leukemia. 35:1586. PubMed
  38. Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
  39. Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
  40. Zhang P, et al. 2021. Nat Med. 22:. PubMed
  41. Aschmoneit N, et al. 2022. Oncoimmunology. 11:2028961. PubMed
  42. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  43. Zhou Z, et al. 2022. J Gastrointest Oncol. 13:732. PubMed
  44. Marquardt I, et al. 2022. Front Microbiol. 12:752549. PubMed
  45. Liu Y, et al. 2022. MAbs. 14:2073632. PubMed
  46. Tsai HI, et al. 2021. EMBO Mol Med. 13:e12834. PubMed
  47. Aschmoneit N, et al. 2021. Sci Rep. 11:13880. PubMed
  48. Zhang Z, et al. 2021. Acta Pharm Sin B. 11:1965. PubMed
  49. Shen C, et al. 2021. BMC Med. 19:283. PubMed
  50. Gardner TJ, et al. 2022. Nat Chem Biol. 18:216. PubMed
  51. Bellini N, et al. 2022. iScience. 25:105234. PubMed
  52. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  53. Thiramanas R, et al. 2020. Cells. 9: . PubMed
  54. Claeys E, et al. 2021. Front Immunol. 12:650731. PubMed
  55. Hagel J, et al. 2021. J Immunol. 206:3073. PubMed
  56. Li X, et al. 2022. J Clin Invest. 132: . PubMed
  57. Zhang H, et al. 2023. Nat Immunol. 24:96. PubMed
  58. Jaiswal A, et al. 2022. Cancer Cell. 40:524. PubMed
  59. Blake D, et al. 2022. Elife. 11:. PubMed
  60. Ainsua-Enrich E, et al. 2022. iScience. 25:105455. PubMed
  61. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  62. Chen J, et al. 2023. Cell Res. 33:341. PubMed
  63. Segaliny AI, et al. 2023. Commun Biol. 6:380. PubMed
  64. Yuan X, et al. 2023. Oncoimmunology. 12:2219544. PubMed
  65. Wang F, et al. 2023. Sci Adv. 9:eadf5464. PubMed
RRID
AB_314840 (BioLegend Cat. No. 310905)
AB_314841 (BioLegend Cat. No. 310906)

Antigen Details

Structure
C-type lectin, type II glycoprotein, 28/32 kD
Distribution

Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells

Function
Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type
B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.

Gene ID
969 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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