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PE anti-human CD80 Antibody

Pricing & Availability
Clone
2D10 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD80.1
Other Names
B7-1, B7, BB1
Isotype
Mouse IgG1, κ
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Product Citations
publications
2D10_PE_070507
Human B-cell Burkitt's lymphoma cell line Daudi stained with 2D10 PE
  • 2D10_PE_070507
    Human B-cell Burkitt's lymphoma cell line Daudi stained with 2D10 PE
See PE spectral data
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305207 25 tests 80€
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305208 100 tests 169€
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Description

CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
  3. Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
  4. Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
  5. Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed
  6. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Werz O, et al. 2018. Nat Commun. 9:59. PubMed
  2. Du X, et al. 2018. Cell Res. 28:416. PubMed
  3. Kubala MH, et al. 2018. Cell Rep. 25:2177. PubMed
  4. Blaszczak AM, et al. 2019. J Diabetes Res. 2019:8124563. PubMed
  5. Gärtner K, et al. 2019. J Extracell Vesicles. 8:1573051. PubMed
  6. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  7. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  8. Longbrake EE, et al. 2018. Mult Scler. 24:728. PubMed
  9. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  10. Zhang L, et al. 2011. Blood. 117:6184. PubMed
  11. Serhan J 2012. Blood. 120:e60. PubMed
  12. Trabanelli S, et al. 2014. J Immunol. 192:1231. PubMed
  13. Herath S, et al. 2014. PLoS One. 9:88327. PubMed
  14. Lathrop S, et al. 2015. Infect Immun . 83: 2661 - 2671. PubMed
  15. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  16. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  17. Wang D, et al. 2016. J Cell Sci. 129: 2261 - 2272. PubMed
  18. Rosskopf S, et al. 2016. Sci Rep. 6:31580. PubMed
  19. Santiago K, et al. 2016. Pathog Dis. 74: ftw081. PubMed
  20. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  21. Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed
  22. Pellegrini JM, et al. 2021. Sci Rep. 11:13559. PubMed
  23. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  24. Ganesan R, et al. 2020. FASEB J. 34:15888. PubMed
  25. Lewis SA, et al. 2021. Front Immunol. 12:724015. PubMed
  26. Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
  27. Morell M, et al. 2022. EBioMedicine. 76:103808. PubMed
  28. Guo Z, et al. 2022. NPJ Precis Oncol. 6:34. PubMed
  29. Hou ZH, et al. 2020. Am J Physiol Cell Physiol. 318:C649. PubMed
  30. Luque-Martin R, et al. 2021. J Immunol. 207:555. PubMed
  31. Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
  32. Corgnac S, et al. 2022. J Immunother Cancer. 10:. PubMed
  33. Costa MHG, et al. 2021. Biotechnol J. 16:e2000389. PubMed
  34. Lertjuthaporn S, et al. 2022. Pathogens. 11: . PubMed
  35. Wu L, et al. 2023. Cell Rep Med. 4:100917. PubMed
  36. Lah S, et al. 2023. J Immunother Cancer. 11:. PubMed
  37. Wang Y, et al. 2023. Hum Vaccin Immunother. 19:2202127. PubMed
  38. Nshimiyimana R, et al. 2023. Am J Hematol. 98:1000. PubMed
RRID
AB_314503 (BioLegend Cat. No. 305207)
AB_314504 (BioLegend Cat. No. 305208)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution

Activated B cells and T cells, macrophages, and dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Freeman G, et al. 1991. J. Exp. Med. 174:625.
2. Linsley P, et al. 1996. Immunity 4:535.
3. Linsley P, et al. 1991. J. Exp. Med. 174:561.

Gene ID
941 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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