PE anti-mouse CD135 Antibody

Product Details

Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse Flt3 transfected cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application References

(PubMed link indicates BioLegend citation)

1. Sergejeva S, et al. 2004. Blood 103:1270.
2. Auffray C, et al. 2009. J. Exp. Med. 206:595.
3. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed

Product Citations

1. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
2. Jackson JT, et al. 2018. Blood Adv. 2:347. PubMed
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5. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
6. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
7. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
8. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
9. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
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11. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
12. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
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14. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
15. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
16. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
17. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
18. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
19. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
20. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
21. Delbridge AR, et al. 2017. Cell Death Dis. 8:e2914. PubMed
22. Viaud M, et al. 2020. Cell Reports. 30(10):3397-3410. PubMed
23. Sabnis A, et al. 2009. PLoS One. 7:e59. PubMed
24. Giotopoulos G, et al. 2015. J Exp Med. 212: 1551-1569. PubMed
25. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
26. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
27. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
28. Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed
29. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
30. Carpenter RS, et al. 2020. Nat Commun. 3.029166667. PubMed
31. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
32. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
33. Dingler FA, et al. 2020. Mol Cell. . PubMed
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35. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
36. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
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38. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
39. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
40. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
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RRID

AB_1877218 (BioLegend Cat. No. 135305)
AB_1877217 (BioLegend Cat. No. 135306)

Antigen Details

Structure

A 135-150 kD molecule belonging to the tyrosine kinase receptor family.

Distribution

Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.

Function

Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.

Ligand/Receptor

Flk-2/FLT3 ligand

Cell Type

B cells, Hematopoietic stem and progenitors

Biology Area

Immunology

Molecular Family

CD Molecules

Antigen References

1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478

Gene ID

14255 View all products for this Gene ID

UniProt

View information about CD135 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?

We use R-PE in our conjugates.

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis