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PE/Cyanine7 anti-human CD45RA Antibody

Pricing & Availability
Clone
HI100 (See other available formats)
Regulatory Status
RUO
Workshop
IV N906
Other Names
GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype
Mouse IgG2b, κ
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Product Citations
publications
HI100_PECy7_011011
Human peripheral blood lymphocytes were stained with HI100 PE/Cyanine7 (filled histogram) or mouse IgG2b, κ PE/Cyanine7 isotype control (open histogram).
  • HI100_PECy7_011011
    Human peripheral blood lymphocytes were stained with HI100 PE/Cyanine7 (filled histogram) or mouse IgG2b, κ PE/Cyanine7 isotype control (open histogram).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
304125 25 tests 102€
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304126 100 tests 260€
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Description

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
  3. Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
  4. Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
  5. Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
  6. Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
  7. Palendira U, et al. 2008. Blood (FC) PubMed
  8. Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
  9. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  10. Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
  11. Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
  14. Canque B, et al. 2000. Blood 96:3748. (ICC)
  15. Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
  16. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  2. BÖiers C, et al. 2018. Dev Cell. 44:362. PubMed
  3. Schulthess J et al. 2019. Immunity. 50(2):432-445 . PubMed
  4. Ollé Hurtado M, et al. 2019. PLoS One. 14:e0216373. PubMed
  5. Collinson–Pautz MR, et al. 2019. Leukemia. 33:2195. PubMed
  6. Kelly DL, et al. 2018. Psychiatry Res. 269:517. PubMed
  7. Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
  8. Bourges C, et al. 2020. EMBO Mol Med. 12:e12112. PubMed
  9. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  10. Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed
  11. Li W, et al. 2020. Immunity. 53(2):456-470. PubMed
  12. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  13. Serwas NK, et al. 2019. Nat Commun. 2.573611111. PubMed
  14. Presicce P, et al. 2011. PLoS One. 6:e28118. PubMed
  15. Hinderer S, et al. 2012. Biomaterials. 33:5259. PubMed
  16. Schilling B, et al. 2014. Ann Oncol. 25:747. PubMed
  17. Koido S, et al. 2014. Clin Cancer Res. 20:4228. PubMed
  18. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  19. Sharei A, et al. 2015. PLoS One. 10:118803. PubMed
  20. Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed
  21. Braza F, et al. 2015. J Am Soc Nephrol. 26: 1795-1805. PubMed
  22. Abdel-Mohsen M, et al. 2016. PLoS Pathog. 12: 1005677. PubMed
  23. Prado-Garcia H, et al. 2017. Cancer Immunol Immunother. 10.1007/s00262-017-1979-x. PubMed
  24. Braun J, et al. 2020. Nature. 587:270. PubMed
  25. Pierini S, et al. 2020. JCI Insight. 5:00. PubMed
  26. Mathiasen SL, et al. 2021. Cell Reports. 35(10):109220. PubMed
  27. Basar R, et al. 2021. Cell Reports. 36(3):109432. PubMed
  28. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  29. Imura Y, et al. 2020. JCI Insight. :5. PubMed
  30. Basar R, et al. 2020. bioRxiv. . PubMed
  31. Robinson GA, et al. 2021. EBioMedicine. 103243:. PubMed
  32. Rubio-Pérez C, et al. 2021. Nat Commun. 1503:12. PubMed
  33. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  34. Sananez I, et al. 2021. EBioMedicine. 72:103615. PubMed
  35. Gamradt S, et al. 2021. iScience. 24:103312. PubMed
  36. Yang L, et al. 2020. Genes Dis. 7:128. PubMed
  37. Jiang XL, et al. 2021. Nat Commun. 12:897. PubMed
  38. Andonian BJ, et al. 2022. Sci Rep. 12:7450. PubMed
  39. Salumets A, et al. 2022. Aging Cell. 21:e13607. PubMed
  40. Qiu J, et al. 2022. STAR Protoc. 3:101828. PubMed
  41. Leick MB, et al. 2022. Cancer Cell. 40:494. PubMed
  42. Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed
  43. Chen J, et al. 2023. Cell Res. 33:341. PubMed
  44. Xiong H, et al. 2023. EBioMedicine. 90:104507. PubMed
RRID
AB_10709440 (BioLegend Cat. No. 304125)
AB_10708879 (BioLegend Cat. No. 304126)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution

B cells, naïve T cells, monocytes

Function
Enhances TCR and BCR signaling
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RA on UniProt.org
Go To Top Version: 2    Revision Date: 07.25.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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