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PerCP anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_PerCP_020608
Human peripheral blood lymphocytes stained with HIB19 PerCP
  • HIB19_PerCP_020608
    Human peripheral blood lymphocytes stained with HIB19 PerCP
See PerCP spectral data
Cat # Size Price Quantity Check Availability Save
302227 25 tests 123€
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302228 100 tests 268€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Meraviglia S, et al. 2017. Oncoimmunology. 6:e1347742. PubMed
  2. Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed
  3. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  4. Salazar-Camarena DC, et al. 2020. Sci Rep. 10:6236. PubMed
  5. Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
  6. Mahmud FJ, et al. 2020. J Neuroinflammation. 0.897916667. PubMed
  7. Zhu Y, et al. 2012. PLoS One. 7:e44131. PubMed
  8. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  9. Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed
  10. Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
  11. Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
  12. Hardman CS, et al. 2021. Sci Immunol. 6:. PubMed
  13. Carre C, et al. 2021. iScience. 24:102970. PubMed
  14. Den Braanker H, et al. 2021. Front Immunol. 12:768113. PubMed
  15. Pandey M, et al. 2021. Clin Transl Immunology. 10:e1260. PubMed
  16. Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
  17. Hu X, et al. 2023. Nat Commun. 14:2020. PubMed
  18. Hu X, et al. 2023. Nat Biotechnol. . PubMed
RRID
AB_893276 (BioLegend Cat. No. 302227)
AB_893272 (BioLegend Cat. No. 302228)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 12.18.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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