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PerCP/Cyanine5.5 anti-human CD16 Antibody

Pricing & Availability
Clone
3G8 (See other available formats)
Regulatory Status
RUO
Workshop
V NK80
Other Names
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype
Mouse IgG1, κ
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Product Citations
publications
3G8_PerCPCyanine55_CD16_Antibody_081718
Human peripheral blood lymphocytes were stained with CD56 APC and CD16 (Clone 3G8) PerCP/Cyanine5.5 (left), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • 3G8_PerCPCyanine55_CD16_Antibody_081718
    Human peripheral blood lymphocytes were stained with CD56 APC and CD16 (Clone 3G8) PerCP/Cyanine5.5 (left), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
302027 25 tests 141€
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302028 100 tests 306€
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Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details
Technical data sheet

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PMN cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
  4. Hoshino S, et al. 1991. Blood 78:3232. (Stim)
  5. Tamm A, et al. 1996. Immunol. 157:1576. (Block)
  6. Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
  7. Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
  8. Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
  9. Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
  10. Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed
  11. Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  14. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  15. Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
  16. Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  18. Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)
Product Citations
  1. Shoda H, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1308-y. PubMed
  2. McDonnell A, et al. 2017. BMC Cancer . 10.1186/s12885-017-3403-5. PubMed
  3. Liu Y, et al. 2017. Oncogene. 10.1038/onc.2017.209. PubMed
  4. Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
  5. Shehata L, et al. 2019. Nat Commun. 10:1126. PubMed
  6. Kuranda K, et al. 2018. J Clin Invest. 128:5267. PubMed
  7. Mishra A, et al. 2018. J Allergy Clin Immunol. 142:1066. PubMed
  8. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  9. Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed
  10. Yeung Y, et al. 2016. Nat Commun. 7:13376. PubMed
  11. Hood SP, et al. 2020. Elife. 9:00. PubMed
  12. Hernndez DC, et al. 2021. Immunity. :. PubMed
  13. Jankeel A, et al. 2020. J Virol. :94. PubMed
  14. Panwar B, et al. 2021. Genome Res. 31:659. PubMed
  15. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  16. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  17. Zhang X, et al. 2021. Front Immunol. 12:602492. PubMed
  18. Lende SSF, et al. 2022. Front Cell Infect Microbiol. 12:919097. PubMed
  19. Murphy DM, et al. 2022. J Clin Invest. Online ahead of print. PubMed
  20. Sun C, et al. 2022. Nat Biomed Eng. 6:1004. PubMed
  21. Rodda LB, et al. 2022. Cell. 185:1588. PubMed
  22. Saavedra D, et al. 2022. Immunol Lett. :1. PubMed
  23. Hopke A, et al. 2022. iScience. 25:105226. PubMed
  24. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  25. Wu X, et al. 2023. iScience. 26:106559. PubMed
RRID
AB_893263 (BioLegend Cat. No. 302027)
AB_893262 (BioLegend Cat. No. 302028)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.

Gene ID
2214 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 4    Revision Date: 08.17.2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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