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PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
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Product Citations
publications
RA3-6B2_PerCPCyanine55_CD45R-B220_Antibody_091919.png
C57BL/6 mouse splenocytes stained with CD3 APC and CD45R/B220 (clone RA3-6B2) PerCP/Cyanine5.5 (left) or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right)
  • RA3-6B2_PerCPCyanine55_CD45R-B220_Antibody_091919.png
    C57BL/6 mouse splenocytes stained with CD3 APC and CD45R/B220 (clone RA3-6B2) PerCP/Cyanine5.5 (left) or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right)
See PerCP/Cyanine5.5 spectral data
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103235 25 µg 72€
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103236 100 µg 196€
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse,Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  2. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  3. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  4. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  5. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  6. Beloor J et al. 2018. Cell host & microbe. 23(4):549-556 . PubMed
  7. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  8. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  9. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
  10. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  11. Melissa M Remy et al. 2017. Cell host & microbe. 22(3):354-365 . PubMed
  12. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  13. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  14. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  15. Page N, et al. 2018. Immunity. 48:937. PubMed
  16. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  17. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  18. Hilpert C, et al. 2019. J Immunol. 203:3068. PubMed
  19. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  20. Leal AS, et al. 2019. Sci Rep. 5.286111111. PubMed
  21. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  22. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  23. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  24. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  25. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  26. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  27. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  28. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  29. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  30. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  31. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  32. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  33. Rivadeneira DB, et al. 2020. Immunity. 51(3):548-560. PubMed
  34. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
  35. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  36. Pereira J, et al. 2010. PLoS One. 5:e9277. PubMed
  37. Sasaki I, et al. 2012. Blood. 120:4733. PubMed
  38. Liu B, et al. 2013. Development. 140:780. PubMed
  39. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  40. Kaku H, et al. 2014. J Immunol. 193:5904. PubMed
  41. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  42. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  43. Kong S, et al. 2015. J Immunol. 195: 3685 - 3693. PubMed
  44. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  45. Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed
  46. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  47. Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed
  48. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  49. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  50. Yang J, et al. 2020. Nature. 586:572. PubMed
  51. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  52. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  53. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  54. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  55. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  56. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  57. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  58. Giampazolias E, et al. 2021. Cell. . PubMed
  59. Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed
  60. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  61. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  62. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  63. Pan H, et al. 2020. Mol Psychiatry. . PubMed
  64. Zhang L, et al. 2020. Cell. 442:181. PubMed
  65. Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed
  66. Dong S, et al. 2021. Nature. 591:117. PubMed
  67. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  68. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
  69. Srinivasan A, et al. 2021. BMC Immunol. 22:46. PubMed
  70. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  71. Shibad V, et al. 2021. Front Immunol. 12:722451. PubMed
  72. Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
  73. Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
  74. Vijay R, et al. 2021. J Exp Med. 218:. PubMed
  75. Neckermann P, et al. 2021. Front Immunol. 12:761214. PubMed
  76. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  77. Xing Y, et al. 2022. Cancer Lett. 529:100. PubMed
  78. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  79. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  80. Li Y, et al. 2021. Front Cell Dev Biol. 9:646077. PubMed
  81. Feng J, et al. 2021. iScience. 24:103004. PubMed
  82. Webb ER, et al. 2022. iScience. 25:104995. PubMed
  83. He C, et al. 2022. Nat Commun. 13:5459. PubMed
  84. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
  85. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  86. Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
  87. Wang JC, et al. 2022. Elife. 11:. PubMed
  88. Zenke S, et al. 2022. Nat Commun. 13:6459. PubMed
  89. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  90. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  91. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  92. Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed
  93. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  94. Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed
  95. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
  96. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  97. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  98. Zong P, et al. 2022. Nat Cardiovasc Res. 1:344. PubMed
  99. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  100. Kuang C, et al. 2023. Nat Commun. 14:2093. PubMed
RRID
AB_893356 (BioLegend Cat. No. 103235)
AB_893354 (BioLegend Cat. No. 103236)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 09.19.2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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