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Purified anti-human CD27 Antibody

Pricing & Availability
Clone
O323 (See other available formats)
Regulatory Status
RUO
Workshop
IV T-186
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
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Product Citations
publications
O323_Purified_062107
Human peripheral blood lymphocytes stained with purified O323, followed by anti-mouse IgGs FITC
  • O323_Purified_062107
    Human peripheral blood lymphocytes stained with purified O323, followed by anti-mouse IgGs FITC
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302802 100 µg 36€
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Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical data sheet

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Magnani D, et al. 2017. PLoS Pathogens. 11(6):e0005655. PubMed
  2. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  3. Crome SQ, et al. 2017. Nat Med. 1.213888889. PubMed
  4. Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
  5. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  6. Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed
  7. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  8. Cole C, et al. 2018. Nucleic Acids Res. 46:e62. PubMed
  9. Renand A, et al. 2018. J Allergy Clin Immunol. 141:1750. PubMed
  10. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  11. Majri SS, et al. 2018. J Immunol. 200:110. PubMed
  12. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  13. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  14. Schwabenland M, et al. 2021. Immunity. . PubMed
  15. Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed
  16. Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed
  17. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  18. Dubrovsky L, et al. 2020. MBio. 11:. PubMed
  19. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  20. Feyaerts D, et al. 2022. Cell Rep Med. 3:100680. PubMed
  21. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
RRID
AB_314294 (BioLegend Cat. No. 302802)

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution

Medullary thymocytes, T and B cell subsets, NK cells

Function
T cell costimulation
Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hintzen R, et al. 1994. Immunol. Today 15:307.
2. Agematsu K, et al. 1995. J. Immunol. 154:3627.

Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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