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Purified anti-human CD28 Antibody

Pricing & Availability
Clone
CD28.2 (See other available formats)
Regulatory Status
RUO
Workshop
V-CD28.05
Other Names
T44, Tp44
Isotype
Mouse IgG1, κ
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Product Citations
publications
28.2
Human peripheral blood lymphocytes stained with CD28.2 PE
  • 28.2
    Human peripheral blood lymphocytes stained with CD28.2 PE
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302901 25 µg 20€
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302902 100 µg 40€
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Description

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.

Product Details
Technical data sheet

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, Costim, FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Nunes J, et al. 1993. Biochem. J. 293:835.
  3. Calea-Lauri J, et al. 1999. J. Immunol. 163:62.
  4. Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC)
  5. Marti F, et al. 2001. J. Immunol. 166:197. (Costim)
  6. Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim)
  7. Rivollier A, et al. 2004. Blood 104:4029. (Costim)
  8. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim)
  9. Sheng W, et al. 2007.Elsevier 580:6819. PubMed
  10. Mitsuhashi M. 2007. Clin Chem.53:148. PubMed
  11. Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed
  12. Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed
  13. Yoshino N, et al. 2008. Exp. Anim. (Tokyo) 49:97. (FC)
  14. Berg M, et al. 2008. J Leukoc Biol. 83:853. (IP) PubMed
  15. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  16. Leonard JA, et al. 2011. J. Virol. 85:6867. PubMed
  17. Nomura T, et al. 2012. J. Virol. 86:6481. PubMed
Product Citations
  1. Jones N, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01516. PubMed
  2. Qiu H, et al. 2017. Exp Cell Res.. 10.1016/j.yexcr.2017.08.036. PubMed
  3. Jin Y, et al. 2017. Int Immunopharmacol. . 10.1016/j.intimp.2017.10.009. PubMed
  4. Pawlak EN, et al. 2018. Retrovirology. 15:6. PubMed
  5. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  6. Pardons M, et al. 2019. PLoS Pathog. 15:e1007619. PubMed
  7. Zhu Y, et al. 2019. Front Genet. 10:141. PubMed
  8. Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
  9. Zhang Y, et al. 2019. Cell Res. 29:609. PubMed
  10. Mazer M, et al. 2019. J Immunol. 203:2088. PubMed
  11. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  12. Bantug GR, et al. 2018. Immunity. 48:542. PubMed
  13. Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
  14. Fasbender F, et al. 2017. Front Immunol. 0.88125. PubMed
  15. Guo T, et al. 2018. J Immunol. 200:500. PubMed
  16. Gee MH, et al. 2018. Cell. 172:549. PubMed
  17. Dias J, et al. 2018. Proc Natl Acad Sci U S A. 115:E11513. PubMed
  18. Jin H, et al. 2019. MBio. 10:e01769-19. PubMed
  19. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  20. Seo N, et al. 2018. Nat Commun. 9:435. PubMed
  21. Liang J, et al. 2017. Nat Commun. 8:15732. PubMed
  22. Zucchetti AE, et al. 2019. Nat Commun. 10:2864. PubMed
  23. Bluhm J, et al. 2018. Mol Ther. 26:1906. PubMed
  24. Bobardt M, et al. 2020. PLoS One. 15:e0227715. PubMed
  25. Holbrook AK, et al. 2019. Physiol Rep. 7:e14234. PubMed
  26. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  27. Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
  28. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  29. Mousset CM, et al. 2018. Oncoimmunology. 7:e1488565. PubMed
  30. Oberg HH, et al. 2020. J Leukoc Biol. 107:1081. PubMed
  31. Sheng WY, et al. 2006. FEBS Lett. 580:6819. PubMed
  32. Mitsuhashi M 2007. Clin Chem. 53:148. PubMed
  33. Ye Z, et al. 2008. Infect Immun. 76:2541. PubMed
  34. Leonard J, et al. 2011. J Virol. 85:6867. PubMed
  35. Nomura T, et al. 2012. J Virol. 86:6481. PubMed
  36. Haas R, et al. 2015. PLoS Biol. 13: 1002202. PubMed
  37. Liu L, et al. 2016. Nat Biotechnol. 10.1038/nbt.3461. PubMed
  38. Barzilai S, et al. 2017. Cell Rep. 18(3):685-699. PubMed
  39. Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed
  40. Zavidij O, et al. 2020. Nat Cancer. 0.384027778. PubMed
  41. Hu X, et al. 2021. British Journal of Pharmacology. 178(6):1445-1458. PubMed
  42. Cai J, et al. 2021. eLife. 10:00. PubMed
  43. He Y, et al. 2021. eLife. 10:00. PubMed
  44. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  45. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  46. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  47. Nathan A, et al. 2021. Cell. 184(17):4401-4413.e10. PubMed
  48. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  49. Martin E, et al. 2020. JCI Insight. :5. PubMed
  50. Panigrahi S, et al. 2020. PLoS Pathog. e1008885:16. PubMed
  51. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  52. Ito T, et al. 2021. Commun Biol. 4:694. PubMed
  53. Gaa R, et al. 2021. MAbs. 13:1978130. PubMed
  54. Levin SD, et al. 2020. Front Immunol. 10:3086. PubMed
  55. Li H, et al. 2020. Front Immunol. 11:1777. PubMed
  56. Willimsky G, et al. 2021. Elife. 10:. PubMed
  57. Cassioli C, et al. 2021. Front Cell Dev Biol. 9:673446. PubMed
  58. Moosmann C, et al. 2022. STAR Protoc. 3:101031. PubMed
  59. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  60. Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed
  61. Qiu C, et al. 2022. Front Immunol. 12:764949. PubMed
  62. Naranbhai V, et al. 2022. Cell. . PubMed
  63. Peng Q, et al. 2022. EBioMedicine. 77:103904. PubMed
  64. Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed
  65. Wiedeman AE, et al. 2020. J Clin Invest. 130:480. PubMed
  66. Tocheva AS, et al. 2020. J Biol Chem. 295:18036. PubMed
  67. Tsai HI, et al. 2021. EMBO Mol Med. 13:e12834. PubMed
  68. Ren X, et al. 2021. Lab Chip. 21:1527. PubMed
  69. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  70. Mammadli M, et al. 2021. Clin Transl Med. 11:e625. PubMed
  71. Duque JSR, et al. 2022. Nat Commun. 13:3700. PubMed
  72. Bonavia AS, et al. 2022. Front Immunol. 13:940030. PubMed
  73. Meléndez AV, et al. 2022. Cell Mol Life Sci. 79:513. PubMed
  74. Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed
  75. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  76. Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
  77. Li L, et al. 2020. J Transl Med. 18:363. PubMed
  78. Tocheva AS, et al. 2020. J Biol Chem. 295:18036. PubMed
  79. Jakob M, et al. 2020. Cells. 10:. PubMed
  80. Umeda M, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  81. Deng J, et al. 2021. J Leukoc Biol. 110:1171. PubMed
  82. Pan Y, et al. 2021. Br J Pharmacol. 178:4907. PubMed
  83. Li G, et al. 2021. Oncoimmunology. 10:1983306. PubMed
  84. Xiao C, et al. 2022. iScience. 25:103934. PubMed
  85. Kondo H, et al. 2022. Front Immunol. 13:836923. PubMed
  86. Liu Y, et al. 2022. Micromachines (Basel). 13:. PubMed
  87. Kim SJ, et al. 2020. J Biol Chem. 296:100108. PubMed
  88. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  89. Saito T, et al. 2022. ACS Chem Biol. 17:217. PubMed
  90. Toothaker JM, et al. 2022. Development. 149: . PubMed
  91. Deepak HB, et al. 2022. Indian J Pharmacol. 54:183. PubMed
  92. Kim HS, et al. 2023. Bioact Mater. 21:241. PubMed
  93. Onnis A, et al. 2023. J Exp Med. 220: . PubMed
  94. Chai H, et al. 2023. Nat Commun. 14:213. PubMed
  95. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  96. Mohamed H, et al. 2023. Biomedicines. 11: . PubMed
  97. Schnizer C 2022. Frontiers in Immunology. 13:1095129. PubMed
  98. Davé E, et al. 2023. MAbs. 15:2160229. PubMed
  99. Pymm P, et al. 2022. Cell Rep. 38:110449. PubMed
  100. Giles JR, et al. 2022. Immunity. 55:557. PubMed
  101. Duan LJ, et al. 2022. Cell Rep. 40:111284. PubMed
  102. Chen L, et al. 2023. Immunology. 169:204. PubMed
  103. Xiao C, et al. 2023. Nat Aging. 3:418. PubMed
  104. Meadows AM, et al. 2023. iScience. 26:106578. PubMed
  105. Menevse AN, et al. 2023. Acta Neuropathol Commun. 11:75. PubMed
  106. Eberhardt F, et al. 2023. Int J Mol Med. 52:. PubMed
RRID
AB_314303 (BioLegend Cat. No. 302901)
AB_314304 (BioLegend Cat. No. 302902)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution

Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells

Function
T cell costimulation
Ligand/Receptor
CD80, CD86
Cell Type
B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. June CH, et al. 1994. Immunol. Today 15:321.
3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.

Gene ID
940 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org
Go To Top Version: 3    Revision Date: 08.25.2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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