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Purified anti-human CD4 Antibody

Pricing & Availability
Clone
RPA-T4 (See other available formats)
Regulatory Status
RUO
Workshop
IV T114
Other Names
T4
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA-T4_Purified_060507
Human peripheral blood lymphocytes stained with purified RPA-T4, followed by anti-mouse IgGs FITC
  • RPA-T4_Purified_060507
    Human peripheral blood lymphocytes stained with purified RPA-T4, followed by anti-mouse IgGs FITC
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300501 25 µg 20€
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300502 100 µg 32€
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Activ, Block, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.  This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed
  2. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  3. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  4. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  5. Liu J, et al. 2018. Cell Physiol Biochem. 45:1034. PubMed
  6. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  7. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  8. Kessel C, et al. 2017. Arthritis Rheumatol. 69:1480. PubMed
  9. Zhao J, et al. 2017. Sci Immunol. 2:eaan5400. PubMed
  10. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  11. Gee MH, et al. 2018. Cell. 172:549. PubMed
  12. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  13. Meckiff BJ, et al. 2019. J Immunol. 203:1276. PubMed
  14. Sutherland APR, et al. 2019. Sci Rep. 9:15302. PubMed
  15. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  16. Shahine A, et al. 2019. Nat Commun. 10:56. PubMed
  17. Willing A, et al. 2018. J Immunol. 200:974. PubMed
  18. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  19. Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed
  20. Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
  21. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  22. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  23. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  24. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  25. Mo Y, et al. 2021. STAR Protocols. 2(2):100453. PubMed
  26. Henrick BM, et al. 2021. Cell. . PubMed
  27. Schwabenland M, et al. 2021. Immunity. . PubMed
  28. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  29. Salazar Y, et al. 2020. J Clin Invest. 130:3560. PubMed
  30. Cosgrove PR, et al. 2021. Pediatr Res. . PubMed
  31. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  32. Senosain MF, et al. 2021. Sci Rep. 11:14424. PubMed
  33. Campbell GR, et al. 2021. Theranostics. 11:9009. PubMed
  34. Peng T, et al. 2021. Front Immunol. 12:735643. PubMed
  35. Olety B, et al. 2021. Sci Adv. 7:eabj7398. PubMed
  36. Levin SD, et al. 2020. Front Immunol. 10:3086. PubMed
  37. Li H, et al. 2020. Front Immunol. 11:1777. PubMed
  38. Chakhtoura M, et al. 2021. PLoS Pathog. 17:e1009732. PubMed
  39. Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
  40. Anna F, et al. 2021. J Immunother Cancer. 9:. PubMed
  41. Ford ES, et al. 2021. J Clin Invest. 131:. PubMed
  42. Jeyanathan M, et al. 2022. JCI Insight. 7:. PubMed
  43. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  44. Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed
  45. Jakob M, et al. 2020. Cells. 10:. PubMed
  46. Liu X, et al. 2022. STAR Protoc. 3:101310. PubMed
  47. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  48. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  49. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  50. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  51. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
RRID
AB_314069 (BioLegend Cat. No. 300501)
AB_314070 (BioLegend Cat. No. 300502)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 5    Revision Date: 08.23.2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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