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Purified anti-mouse TCR γ/δ Antibody

Pricing & Availability
Clone
GL3 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor γ/δ
Isotype
Armenian Hamster IgG
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Product Citations
publications
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118101 100 µg 95€
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, which is involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. γ/δ TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8- although some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
C57BL/6J intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The GL3 antibody has been shown to be useful in identifying γ/δ T cells by flow cytometry and immunohistochemistry and depleting γ/δ T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ T cells3-5.

Application References

(PubMed link indicates BioLegend citation)
  1. Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
  2. Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
  3. Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
  4. Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
  5. Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
  6. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  7. Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
  8. Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
  9. Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
Product Citations
  1. Mantri CK, et al. 2019. J Clin Invest. 129:1094. PubMed
  2. Castro‐Dopico T et al. 2019. Immunity. 50(4):1099-1114 . PubMed
  3. Shapiro MJ, et al. 2019. J Immunol. 202:2287. PubMed
  4. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  5. Waickman AT, et al. 2017. Cytokine. 99:266. PubMed
  6. Biljes D, et al. 2017. EXCLI J. 0.86875. PubMed
  7. Yoshizaki T, et al. 2019. Sci Rep. 9:17067. PubMed
  8. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
  9. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  10. Mensurado S, et al. 2018. PLoS Biol. 16:e2004990. PubMed
  11. Yoon BH, et al. 2018. Mol Cells. 41:953. PubMed
  12. Suhail A, et al. 2019. Cell Rep. 29:3522. PubMed
  13. Castro–Dopico T, et al. 2020. Cell Reports. 32(1):107857. PubMed
  14. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
  15. Kasten K, et al. 2010. Infect Immun. 78:4714. PubMed
  16. Stadanlick J, et al. 2011. J Immunol. 187:664. PubMed
  17. Bréart B, et al. 2011. J Exp Med. 208:1267. PubMed
  18. Belle A, et al. 2012. J Immunol. 188:462. PubMed
  19. Díaz NM, et al. 2020. Invest Ophthalmol Vis Sci. 61:37:00. PubMed
  20. Dasgupta D, et al. 2020. Gastroenterology. 159:1487. PubMed
  21. Álvarez-Salamero C, et al. 2020. PLoS Biol. 18:e3000646. PubMed
  22. Park S, et al. 2021. Nat Cell Biol. 23:476. PubMed
  23. Ma Z, et al. 2021. Cancer Med. 10:5358. PubMed
  24. Nobs SP, et al. 2021. J Allergy Clin Immunol. . PubMed
  25. Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
  26. Cardoso F, et al. 2021. Nature. 597:410. PubMed
  27. Bittner-Eddy PD, et al. 2020. Front Immunol. 11:677. PubMed
  28. Zhang MH, et al. 2020. Front Immunol. 11:560. PubMed
  29. Lopes N, et al. 2021. Nat Immunol. 22:179. PubMed
  30. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  31. Goodall KJ, et al. 2021. J Biol Chem. 297:101141. PubMed
  32. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  33. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
RRID
AB_313826 (BioLegend Cat. No. 118101)

Antigen Details

Structure
Ig superfamily, associates with CD3 complex.
Distribution

T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.

Function
Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
Ligand/Receptor
Some bacterial or tumor antigens bound to MHC class I.
Cell Type
Epithelial cells, T cells, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References
  1. Skarstein K, et al. 1995. Immunology. 81:497.
  2. Harrison LC, et al. 1996. J Exp Med. 184:2167.
  3. Wildner G, et al. 1996. Eur J Immunol. 26:2140.
  4. Brandes M, et al. 2005. Science. 309:264.
Gene ID
110066 View all products for this Gene ID 110067 View all products for this Gene ID
UniProt
View information about TCR gamma/delta on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

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