Ultra-LEAF™ Purified anti-human CD279 (PD-1) Antibody

Product Details

Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding^1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections¹³, and spatial biology (IBEX)^15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)

1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
3. Velu V, et al. 2007. J. Virol. 81:5819. (FA)
4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
12. Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
13. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
14. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
15. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
16. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
2. Moon E, et al. 2016. Clin Cancer Res. 22: 436 - 447. PubMed
3. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
4. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
5. Martínez M, et al. 2021. Oncoimmunology. 10:1873607. PubMed
6. Zaghi E, et al. 2021. JCI Insight. 6:. PubMed
7. Duraiswamy J, et al. 2021. Cancer Cell. 39:1623. PubMed
8. Yang Z, et al. 2022. Cancer Discov. 12:1942. PubMed
9. Wischnewski V, et al. 2023. Nat Cancer. . PubMed
10. Zhang C, et al. 2023. Oncoimmunology. 2261242:12. PubMed

RRID

AB_11147369 (BioLegend Cat. No. 329925)
AB_11147365 (BioLegend Cat. No. 329926)
AB_2783202 (BioLegend Cat. No. 329957)
AB_2783203 (BioLegend Cat. No. 329958)
AB_2783204 (BioLegend Cat. No. 329959)
AB_2783205 (BioLegend Cat. No. 329960)

Antigen Details

Structure

Immunoglobulin superfamily

Distribution

Transiently expressed on CD4^- CD8^- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells

Ligand/Receptor

B7-H1 (also known as PD-L1) and B7-DC (PD-L2)

Cell Type

B cells, Lymphocytes, T cells, Thymocytes, Tregs

Biology Area

Cancer Biomarkers, Immunology, Inhibitory Molecules

Molecular Family

CD Molecules, Immune Checkpoint Receptors

Gene ID

5133 View all products for this Gene ID

UniProt

View information about CD279 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis