- Clone
- RPA-T8 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV T171
- Other Names
- T8, Leu2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RPA-T8_A594_CD8_Antibody_1_102820 RPA-T8_A594_CD8_Antibody_1_102820](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RPA-T8_A594_CD8_Antibody_1_102820.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml anti-human CD8 (clone RPA-T8) Alexa Fluor® 594 (red) and 5 µg/ml anti-human CD4 (clone RPA-T4) Alexa Fluor® 488 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI and are shown in blue. The image was captured by 40X objective. -
Human peripheral blood lymphocytes were stained with CD8 (clone RPA-T8) Alexa Flour® 594 (filled histogram) or mouse IgG1, κ Alexa Flour® 594 isotype control (open histogram). The data was acquired by BD LSRFortessa™ cell analyzer equipped with the Yellow-Green Laser (561 nm).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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301056 | 100 µg | £182 |
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Chimpanzee, Baboon, Pigtailed Macaque, Sooty Mangabey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
FC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).
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Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
- Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Product Citations
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- RRID
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AB_2563232 (BioLegend Cat. No. 301056)
Antigen Details
- Structure
- Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
- Distribution
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Majority of thymocytes, T cell subset, NK cells
- Function
- MHC class I co-receptor, thymic differentiation, T cell activation
- Ligand/Receptor
- MHC Class I molecules
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD8alpha
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD8a
- App Abbreviation (DOES NOT SHOW ON TDS):
- ICC,FC
- UniProt
- View information about CD8alpha on UniProt.org
Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 APC -
APC/Cyanine7 anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Biotin anti-human CD8a
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 FITC -
PE anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 PE Human peripheral blood was stained with CD8a (clone RPA-T8) ... -
PE/Cyanine5 anti-human CD8a
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PE/Cyanine7 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 PE/Cy... -
Purified anti-human CD8a
Human peripheral blood lymphocytes stained with purified RPA... -
Alexa Fluor® 488 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 Alexa... -
Alexa Fluor® 647 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 Alexa... -
Pacific Blue™ anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 Pacif... -
Alexa Fluor® 700 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 Alexa... -
PerCP anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 PerCP -
PerCP/Cyanine5.5 anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 APC... -
Brilliant Violet 421™ anti-human CD8a
Human peripheral blood mononuclear cells were fixed with 2% ... Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 570™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 and... -
Brilliant Violet 605™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 650™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 711™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 785™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 510™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Purified anti-human CD8a (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 146Nd-an... -
Alexa Fluor® 594 anti-human CD8a
Human peripheral mononuclear cells were fixed with 2% parafo... Human peripheral blood lymphocytes were stained with CD8 (cl... -
PE/Dazzle™ 594 anti-human CD8a
Human peripheral blood lymphocytes were stained with CD8 (cl... -
APC/Fire™ 750 anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FIT... -
TotalSeq™-A0080 anti-human CD8a
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TotalSeq™-B0080 anti-human CD8a
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TotalSeq™-C0080 anti-human CD8a
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Ultra-LEAF™ Purified anti-human CD8a
Human peripheral blood lymphocytes stained with LEAF™ purifi... -
Spark Violet™ 423 anti-human CD8a Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD8a
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark PLUS UV™ 395 anti-human CD8a
Human peripheral blood lymphocytes were stained with anti-hu...
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