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APC anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
XMG1.2 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Rat IgG1, κ
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Product Citations
publications
XMG1dot2_APC_032808
PMA/Ionomycin-stimulated (6hrs) C57BL/6 mouse splenocytes stained with XMG1.2 APC and B220 (RA3-6B2) PE
  • XMG1dot2_APC_032808
    PMA/Ionomycin-stimulated (6hrs) C57BL/6 mouse splenocytes stained with XMG1.2 APC and B220 (RA3-6B2) PE
Compare all formats See APC spectral data
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505809 25 µg £31
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505810 100 µg £91
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Description

IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IFN-γ antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.
ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).
Flow Cytometry7,8,12,13,16: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.
Neutralization1-3,9,10: The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.
Note: For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut)
  2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA, Neut)
  3. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA, Neut)
  4. Yang X, et al. 1993. J. Immunoassay 14:129. (ELISA)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT)
  6. Sander B, et al. 1991. Immunol. Rev. 119:65. (IHC)
  7. Ferrick D, et al. 1995. Nature 373:255. (FC)
  8. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  9. Peterson KE, et al. 2000. J. Virol. 74:5363. (Neut)
  10. DeKrey GK, et al. 1998. Infect. Immun. 66:827. (Neut)
  11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
  12. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  13. Lee JW, et al. 2006. Nature Immunol. 8:181. (FC) PubMed
  14. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
  15. Montfort M, et al.2004. J. Immunol. 173:4084. PubMed
  16. Haring JS, et al. 2008. J. Immunol. 180:2855. (FC) PubMed
  17. Jordan JM, et al. 2008. Infect Immun. 76:3717. PubMed
  18. Tonkin DR, et al. 2008. J. Immunol. 181:4516. PubMed
  19. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  20. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed
  21. Mykkanen OT, et al. 2014. PLoS One. 9:114790. PubMed
  22. Yokogawa M, et al. 2013. Mol. Carcinog. 52:760. (IHC)
  23. Mottram PL, et al. 1998. J Immunol. 161:602. (IHC)
Product Citations
  1. Oliver AJ, et al. 2020. Oncoimmunology. 9:1802979. PubMed
  2. Zhou R, et al. 2022. EBioMedicine. 75:103762. PubMed
  3. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  4. Bergot AS, et al. 2020. J Immunol. 204:1787. PubMed
  5. Tian WJ, et al. 2021. Clin Exp Pharmacol Physiol. 48:902. PubMed
  6. Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed
  7. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  8. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  9. Stone ET, et al. 2022. Cell Rep. 38:110388. PubMed
  10. Guttman O, et al. 2022. J Cell Biol. 221: . PubMed
  11. Qu L, et al. 2022. Cell. 185:1728. PubMed
  12. Grebinoski S, et al. 2022. Nat Immunol. 23:868. PubMed
  13. Guo X, et al. 2022. Invest Ophthalmol Vis Sci. 63:12. PubMed
  14. Blanas A, et al. 2022. iScience. 25:104719. PubMed
  15. Gary EN, et al. 2022. Cell Rep Med. 3:100693. PubMed
  16. Chen C, et al. 2022. Sci Adv. 8:eabo4363. PubMed
  17. Zhang Z, et al. 2022. Mol Nutr Food Res. 66:e2200300. PubMed
  18. Balood M, et al. 2022. Nature. 611:405. PubMed
  19. Zhu M, et al. 2022. Front Immunol. 13:1019248. PubMed
  20. Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed
  21. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  22. Shi C, et al. 2023. Nat Nanotechnol. 18:86. PubMed
  23. Liu P, et al. 2023. Clin Immunol. 246:109212. PubMed
  24. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  25. Xiao J, et al. 2023. J Clin Invest. 133: . PubMed
  26. Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed
  27. Cheng H, et al. 2023. Nat Metab. 5:314. PubMed
  28. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  29. Vijver SV, et al. 2023. Front Immunol. 13:1100730. PubMed
  30. Drijvers JM, et al. 2021. Cancer Immunol Res. 9:184. PubMed
  31. Jiang L, et al. 2021. Nano Today. 36:. PubMed
  32. Tao X, et al. 2022. J Exp Med. 219:. PubMed
  33. McCafferty S, et al. 2022. Mol Ther. 30:2968. PubMed
  34. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  35. Quispe Calla N, et al. 2016. Sci Rep. 6:37723. PubMed
  36. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  37. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  38. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  39. Chinta KC et al. 2018. Cell reports. 25(7):1938-1952 . PubMed
  40. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  41. Zheng X, et al. 2019. PLoS Pathog. 15:e1008036. PubMed
  42. Kisielow J, et al. 2019. Nat Immunol. 1.286111111. PubMed
  43. Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed
  44. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  45. Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed
  46. Wang Z, et al. 2022. J Control Release. 349:929. PubMed
  47. Konjar S, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202144119. PubMed
  48. Barsheshet Y, et al. 2022. Int J Mol Sci. 23:. PubMed
  49. Shi X, et al. 2023. iScience. 26:106143. PubMed
  50. Tursi NJ, et al. 2023. Front Immunol. 14:1072810. PubMed
  51. Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed
  52. Latour YL, et al. 2023. Gut Microbes. 15:2192623. PubMed
  53. Mackin S, et al. 2023. Nat Microbiol. 8:569. PubMed
  54. Fu S, et al. 2023. Nat Commun. 14:2248. PubMed
  55. Fiedler T, et al. 2023. J Neuroinflammation. 20:100. PubMed
  56. Xie J, et al. 2023. Mol Ther Oncolytics. 29:61. PubMed
  57. Seo YB, et al. 2023. Int J Mol Sci. 24:. PubMed
  58. Wang X, et al. 2023. Nat Commun. 14:3440. PubMed
  59. Yang N, et al. 2022. NPJ Vaccines. 7:120. PubMed
  60. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  61. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  62. Hamilton JAG, et al. 2021. Aging Cell. 20:e13309. PubMed
  63. Du C, et al. 2016. Nat Commun. 7: 11120. PubMed
  64. Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
  65. Xie A et al. 2017. Endocrinology. 158(10):3140-3151 . PubMed
  66. Vacca M, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01462. PubMed
  67. Seo YB, et al. 2021. Vaccines (Basel). 9: . PubMed
  68. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  69. Wabitsch S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1166. PubMed
  70. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  71. Muthumani K, et al. 2015. Sci Transl Med. 7: 301ra132. PubMed
  72. Laroche-Lefebvre C, et al. 2016. J Immunol. 197: 3618 - 3627. PubMed
  73. Sanchez-Felipe L, et al. 2021. Nature. 590:320. PubMed
  74. Yang C, et al. 2018. J Immunol. 200:1316. PubMed
  75. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  76. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  77. Diao L, et al. 2022. iScience. 25:105511. PubMed
  78. Guo Q, et al. 2021. Front Cardiovasc Med. 8:633212. PubMed
  79. Gu M, et al. 2021. Nat Immunol. 22:193. PubMed
  80. Yang X, et al. 2021. Front Pharmacol. 12:771046. PubMed
  81. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  82. Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed
  83. Fitzgerald AA, et al. 2021. J Immunother Cancer. 9:. PubMed
  84. Wabitsch S, et al. 2021. STAR Protoc. 2:100517. PubMed
  85. Oda SK, et al. 2020. J Exp Med. 217: . PubMed
  86. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  87. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  88. Zhao Y, et al. 2015. PLoS One. 10: 0134797. PubMed
  89. Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed
  90. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  91. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  92. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  93. Lu J, et al. 2018. ACS Nano. 12:11041. PubMed
  94. Konrath KM, et al. 2022. Cell Rep. 38:110318. PubMed
  95. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  96. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  97. Wang X, et al. 2021. Front Cell Dev Biol. 632805:9. PubMed
  98. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  99. Qi X, et al. 2019. Nat Commun. 10:2141. PubMed
  100. Matsuoka S, et al. 2019. Haematologica. 105:226. PubMed
  101. Saini V, et al. 2020. Nat Commun. 0.845138889. PubMed
  102. Chaurasiya S, et al. 2020. Oncoimmunology. 9:1729300. PubMed
  103. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  104. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  105. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  106. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  107. Han C, et al. 2021. Cell Reports. 34(6):108706. PubMed
  108. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  109. Cabrera-Mora M, et al. 2015. Infect Immun . 83: 3749-3761. PubMed
  110. DeBerge M, et al. 2013. PLoS One. 8:79340. PubMed
  111. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  112. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  113. MacDonald A, et al. 2021. Front Immunol. 12:755995. PubMed
  114. Yang K, et al. 2022. J Clin Invest. 132:. PubMed
  115. Mills C, et al. 2022. Cells. 11:. PubMed
  116. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  117. Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
  118. Li H, et al. 2022. Theranostics. 12:6422. PubMed
  119. Vijay R, et al. 2015. J Exp Med. 212: 1851 - 1868. PubMed
  120. Mingozzi F, et al. 2016. EMBO Mol Med. 8: 1039 - 1051. PubMed
  121. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  122. Gary EN, et al. 2021. iScience. 24(7):102699. PubMed
  123. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  124. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  125. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  126. Lai C, et al. 2009. Invest Ophthalmol Vis Sci. 50:4279. PubMed
  127. Wang W, et al. 2022. Aging Cell. 21:e13630. PubMed
  128. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  129. Marks KE, et al. 2021. Cell Reports. 35(13):109303. PubMed
  130. Zhu XG, et al. 2020. Cell Metabolism. 33(1):211-221.e6. PubMed
  131. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  132. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
  133. Harty J 2009. Infect Immun. 77:1894. PubMed
  134. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  135. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  136. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  137. Luo Y et al. 2019. Cell reports. 26(7):1869-1879 . PubMed
  138. Yang S, et al. 2019. Nat Commun. 10:2782. PubMed
  139. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  140. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  141. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  142. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  143. Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed
  144. Bhattacharya D, et al. 2014. J Biol Chem. 289:16508. PubMed
  145. El-Zaatari M, et al. 2014. J Immunol. 193:807. PubMed
  146. Souza COS, et al. 2021. iScience. 24(6):102548. PubMed
  147. Peng Y 2017. PLoS One. 10.1371/journal.pone.0188112. PubMed
  148. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  149. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  150. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
  151. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  152. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  153. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  154. Yuan Y, et al. 2022. Cell Rep. 38:110256. PubMed
  155. Takeshita Y, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  156. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  157. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  158. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  159. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  160. Ouyang W, et al. 2021. Invest Ophthalmol Vis Sci. 62:25:00. PubMed
  161. Baban B, et al. 2021. JCI Insight. 6:00. PubMed
  162. Knocke S, et al. 2016. Cell Rep. 17:2234-2246. PubMed
  163. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  164. van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
  165. Park JY, et al. 2019. Cell Rep. 27:2548. PubMed
  166. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  167. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  168. Matundan HH, et al. 2021. J Virol. 95:e0103621. PubMed
  169. Zheng D, et al. 2022. Acta Pharm Sin B. 12:2740. PubMed
  170. Li YN, et al. 2022. Nat Commun. 13:4074. PubMed
  171. Yang B, et al. 2022. Bioengineered. 13:2685. PubMed
  172. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  173. Uddback I, et al. 2016. Sci Rep. 6:20137. PubMed
  174. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  175. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  176. Huang F, et al. 2018. Oncoimmunology. 7:e1450713. PubMed
  177. Levesque S, et al. 2019. Oncoimmunology. 8:e1657375. PubMed
  178. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  179. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  180. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  181. Dai R, et al. 2021. Front Immunol. 12:727046. PubMed
  182. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  183. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  184. Chou T, et al. 2016. Nat Commun. 7:11904. PubMed
  185. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  186. Tonkin D, et al. 2008. J Immunol. 181:4516. PubMed
  187. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  188. Ryan NM, et al. 2022. Front Immunol. 13:932742. PubMed
  189. Pandit M, et al. 2021. Eur J Immunol. 51:1461. PubMed
  190. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  191. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  192. Wang H, et al. 2020. Nat Mater. 1.655555556. PubMed
  193. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  194. van Vloten JP, et al. 2019. Mol Ther Methods Clin Dev. 13:154. PubMed
  195. Dudeck J, et al. 2019. J Allergy Clin Immunol. 143:1849. PubMed
  196. Montfort M, et al. 2004. J Immunol. 173:4084. PubMed
  197. Tajima M, et al. 2022. Curr Protoc. 2:e540. PubMed
  198. Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed
  199. Gupta S, et al. 2022. Life Sci. 288:120182. PubMed
  200. Zhang X, et al. 2021. Front Pharmacol. 12:629513. PubMed
  201. Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
  202. Dekhtiarenko I, et al. 2016. PLoS Pathog. 12:e1006072. PubMed
  203. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  204. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  205. Jin R, et al. 2008. J Immunol. 180:2256. PubMed
  206. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  207. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  208. Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed
  209. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  210. Wong YC, et al. 2019. J Virol. 93:e01154-19. PubMed
  211. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  212. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  213. Wu J, et al. 2021. STAR Protoc. 2:101022. PubMed
  214. Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed
  215. Karanika S, et al. 2022. Front Immunol. 13:972266. PubMed
  216. Hartwig S, et al. 2014. PLoS One. 9:90720. PubMed
  217. M H, et al. 2016. Open Bio. 6: 150208. PubMed
  218. Moon J, et al. 2020. Immune Netw. 20:e40. PubMed
  219. Fang Y, et al. 2021. J Clin Invest. 131:00:00. PubMed
  220. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
  221. Kovacs SB, et al. 2021. STAR Protoc. 2:100244. PubMed
  222. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  223. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  224. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  225. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  226. Kung YJ, et al. 2022. J Biomed Sci. 29:57. PubMed
  227. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  228. Kurihara T, et al. 2015. PLoS One. 10: e0139692. PubMed
  229. Flesch I, et al. 2015. J Immunol. 195: 2263-2272. PubMed
  230. Lee J, et al. 2007. Nat Immunol. 8:181. PubMed
  231. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  232. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  233. Neckermann P, et al. 2021. Front Immunol. 12:761214. PubMed
  234. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  235. Byrne K, et al. 2014. J Immunol. 192:1433. PubMed
  236. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  237. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  238. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  239. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  240. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  241. Dokoshi T, et al. 2020. Cell Rep. 30:61. PubMed
  242. Renner K, et al. 2020. Cell Reports. 29(1):135-150.e9.. PubMed
  243. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  244. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  245. Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
  246. Yang N, et al. 2021. NPJ Precis Oncol. 5:37. PubMed
  247. Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
  248. Wang F, et al. 2021. Neoplasia. 23:281. PubMed
  249. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  250. Sheng J, et al. 2021. eLife. 10:00. PubMed
  251. Chen S, et al. 2015. Cancer Res . 7: 519-531. PubMed
  252. Bransi A, et al. 2015. Cancer Immunol Res. 3: 1279 - 1288. PubMed
  253. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  254. Kwak JE, et al. 2019. Nat Commun. 10:3836. PubMed
  255. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  256. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  257. Yang L, et al. 2021. Front Immunol. 12:722273. PubMed
  258. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  259. Tang L, et al. 2022. Front Immunol. 12:770402. PubMed
  260. Li M, et al. 2020. J Immunother Cancer. 8:00. PubMed
  261. Kim SI, et al. 2020. Molecular Cancer Therapeutics. 20(1):173-182. PubMed
  262. Wu L, et al. 2020. Cancer Immunol Res. 710:8. PubMed
  263. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  264. Kalim KW, et al. 2021. Front Immunol. 12:726393. PubMed
  265. Shi GN, et al. 2021. Ther Adv Med Oncol. 13:1758835920987056. PubMed
  266. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  267. Ding X, et al. 2021. Front Immunol. 12:667136. PubMed
  268. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
  269. Muri J, et al. 2020. Cell Reports. 30(13):4399-4417. PubMed
  270. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
RRID
AB_315403 (BioLegend Cat. No. 505809)
AB_315404 (BioLegend Cat. No. 505810)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APCs
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Gene ID
15978 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
IFN-gamma
Specificity Alt (DOES NOT SHOW ON TDS):
IFN-γ
App Abbreviation (DOES NOT SHOW ON TDS):
ICFC
UniProt
View information about IFN-gamma on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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