Loading...

FITC anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
IM7
C57BL/6 splenocytes stained with IM7 FITC
  • IM7
    C57BL/6 splenocytes stained with IM7 FITC
  • IM7_FITC_060710
    Human peripheral blood lymphocytes stained with IM7 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
103021 25 tests £29
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103005 50 µg £33
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103006 500 µg £76
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103022 100 tests £77
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD44 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Yang L, et al. 2022. Front Oncol. 12:862250. PubMed
  2. Goncharov NV, et al. 2022. J Evol Biochem Physiol. 58:230. PubMed
  3. Qu L, et al. 2022. Cell. 185:1728. PubMed
  4. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  5. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  6. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
  7. Yang L, et al. 2023. JCI Insight. 8: . PubMed
  8. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  9. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  10. Ye ZW, et al. 2022. Cell Mol Immunol. 19:588. PubMed
  11. Tan L, et al. 2022. Aging Dis. 13:1562. PubMed
  12. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  13. Wang S, et al. 2019. Int J Oncol. 54:966. PubMed
  14. Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed
  15. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  16. Fan NW, et al. 2023. FASEB J. 37:e22855. PubMed
  17. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  18. Seclì L, et al. 2023. J Immunother Cancer. 11:. PubMed
  19. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  20. Milighetti M, et al. 2023. iScience. 26:106937. PubMed
  21. Di Martile M, et al. 2020. J Immunother Cancer. 8:. PubMed
  22. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  23. Israelow B, et al. 2020. bioRxiv. . PubMed
  24. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  25. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  26. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  27. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  28. Dong L, et al. 2021. Cancer Cell. . PubMed
  29. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  30. Wang D, et al. 2018. Immunity. 48:659. PubMed
  31. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  32. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  33. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  34. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  35. Diao L, et al. 2022. iScience. 25:105511. PubMed
  36. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  37. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  38. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  39. Pobezinskaya EL et al. 2019. Front Immunol. 1.079861111 . PubMed
  40. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  41. Hao J et al. 2018. Cell metabolism. 28(5):689-705 . PubMed
  42. Yang L, et al. 2021. JCI Insight. 6:. PubMed
  43. Bommireddy R, et al. 2020. Vaccines (Basel). 8:. PubMed
  44. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  45. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  46. Kmieciak M, et al. 2011. Breast Cancer Res Treat. 126:385. PubMed
  47. He C, et al. 2020. Clin Transl Med. 10:e39. PubMed
  48. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  49. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  50. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  51. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  52. Bagheri V et al. 2018. Journal of cellular physiology. 233(10):7036-7046 . PubMed
  53. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  54. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  55. Teng ZX, et al. 2022. J Inflamm Res. 15:3187. PubMed
  56. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  57. Murai T, et al. 2013. J Cell Sci. 126:3284. PubMed
  58. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  59. Zhang W, et al. 2021. Cell Prolif. 54:e13058. PubMed
  60. Wang Z, et al. 2022. Ann Transl Med. 10:544. PubMed
  61. Ebina-Shibuya R, et al. 2021. Elife. 10:. PubMed
  62. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  63. Daniel CJ, et al. 2022. Mol Cancer Res. 20:1151. PubMed
  64. Fujiwara H, et al. 2021. Nat Immunol. 22:1440. PubMed
  65. Amici SA, et al. 2021. Front Immunol. 12:695947. PubMed
  66. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  67. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  68. Park SG, et al. 2021. J Vet Sci. 22:e16. PubMed
  69. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  70. Xia H, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L926. PubMed
  71. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  72. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  73. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  74. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  75. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  76. Wu X, et al. 2022. Cell Death Dis. 13:557. PubMed
  77. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  78. Wan X, et al. 2018. Nature. 560:107. PubMed
  79. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  80. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  81. Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed
  82. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  83. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  84. Liu ZZ, et al. 2021. Autophagy. 17:2766. PubMed
  85. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  86. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  87. Sim S, et al. 2016. PLoS One. 11: 0152485. PubMed
  88. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  89. Zhang S, et al. 2021. Stem Cells Int. 6616240:2021. PubMed
  90. Ahmed MF, et al. 2019. Exp Ther Med. 17:4154. PubMed
  91. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  92. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  93. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  94. Liu Z, et al. 2016. Sci Rep. 6:34416. PubMed
  95. Chou T, et al. 2016. Nat Commun. 7:11904. PubMed
  96. Kamijo S, et al. 2013. J Immunol. 190:4489. PubMed
  97. Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
  98. Matossian MD, et al. 2019. BMC Cancer. 19:205. PubMed
  99. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  100. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  101. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  102. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  103. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  104. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  105. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  106. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  107. Geburek F, et al. 2017. Stem Cell Res Ther. 8:129. PubMed
  108. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  109. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  110. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  111. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  112. Wang L, et al. 2021. Sci Adv. 7:eabj4796. PubMed
  113. Reda M, et al. 2022. Nat Commun. 13:4261. PubMed
  114. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  115. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  116. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  117. Witschen PM, et al. 2020. Cancers. 12(5):1325. PubMed
  118. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  119. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  120. Neckermann P, et al. 2021. Front Immunol. 12:761214. PubMed
  121. Byrne K, et al. 2014. J Immunol. 192:1433. PubMed
  122. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  123. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  124. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  125. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  126. Shah D, et al. 2018. Clin Cancer Res. 24:4566. PubMed
  127. Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
  128. Burl RB et al. 2018. Cell metabolism. 28(2):300-309 . PubMed
  129. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  130. Yang L, et al. 2022. Proteomics. 22:e2200018. PubMed
  131. Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
  132. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  133. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  134. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  135. Vogiatzi A, et al. 2021. Mol Cell Biol. 41:e0014921. PubMed
  136. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  137. Zhu Q, et al. 2021. J Am Heart Assoc. 10:e023491. PubMed
  138. Bi R, et al. 2020. Osteoarthritis Cartilage. 28:842. PubMed
  139. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  140. Zhao Y, et al. 2020. Br J Cancer. 1638:122. PubMed
  141. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  142. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
RRID
AB_493684 (BioLegend Cat. No. 103021)
AB_312956 (BioLegend Cat. No. 103005)
AB_312957 (BioLegend Cat. No. 103006)
AB_493685 (BioLegend Cat. No. 103022)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD44
Specificity Alt (DOES NOT SHOW ON TDS):
CD44
App Abbreviation (DOES NOT SHOW ON TDS):
FC,ICC,IHC-P
UniProt
View information about CD44 on UniProt.org

Other Formats

View All CD44 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD44 IM7 FC
Biotin anti-mouse/human CD44 IM7 FC,ICC,IHC-P
FITC anti-mouse/human CD44 IM7 FC,ICC,IHC-P
PE/Cyanine5 anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 IM7 FC,IHC-F,CyTOF®,ELISA,ICC,IHC-P,IP,CMCD,Stim
Brilliant Violet 605™ anti-mouse/human CD44 IM7 FC
PE anti-mouse/human CD44 IM7 FC
Alexa Fluor® 488 anti-mouse/human CD44 IM7 FC,ICC,IHC-P,SB
Alexa Fluor® 647 anti-mouse/human CD44 IM7 FC,ICC,SB
Pacific Blue™ anti-mouse/human CD44 IM7 FC
Alexa Fluor® 700 anti-mouse/human CD44 IM7 FC,SB
PE/Cyanine7 anti-mouse/human CD44 IM7 FC
APC/Cyanine7 anti-mouse/human CD44 IM7 FC
PerCP/Cyanine5.5 anti-mouse/human CD44 IM7 FC
PerCP anti-mouse/human CD44 IM7 FC
Brilliant Violet 421™ anti-mouse/human CD44 IM7 FC,ICC,IHC-P
Brilliant Violet 570™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 785™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 510™ anti-mouse/human CD44 IM7 FC
Ultra-LEAF™ Purified anti-mouse/human CD44 IM7 FC,CyTOF®,ELISA,IHC,IP,CMCD,Stim
Brilliant Violet 650™ anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 (Maxpar® Ready) IM7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD44 IM7 ICC,FC,IHC-P,SB
PE/Dazzle™ 594 anti-mouse/human CD44 IM7 FC
Brilliant Violet 711™ anti-mouse/human CD44 IM7 FC
APC/Fire™ 750 anti-mouse/human CD44 IM7 FC
TotalSeq™-A0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-C0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-B0073 anti-mouse/human CD44 IM7 PG
Spark YG™ 570 anti-mouse/human CD44 IM7 FC,IHC-P
Spark YG™ 593 anti-mouse/human CD44 IM7 FC
TotalSeq™-D0073 anti-mouse/human CD44 IM7 PG
Brilliant Violet 750™ anti-mouse/human CD44 IM7 FC
PerCP/Fire™ 806 anti-mouse/human CD44 IM7 FC
Spark Red™ 718 anti-mouse/human CD44 IM7 FC
PE/Fire™ 810 anti-mouse/human CD44 IM7 FC
Spark Blue™ 550 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Spark Red™ 718 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Go To Top Version: 2    Revision Date: 09/11/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account