- Clone
- 5G8-D3-H7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LMN2, LMNB, ADLD, LMN
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![5G8-D3-H7_PURE_Lamin-B1_Antibody_1_102319 5G8-D3-H7_PURE_Lamin-B1_Antibody_1_102319](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/5G8-D3-H7_PURE_Lamin-B1_Antibody_1_102319.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-Lamin B1 antibody (clone 5G8-D3-H7). Lane 1: Molecular weight marker; Lane 2: 30 µg of human cortex lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 5 µg of HeLa cell lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D3-H7) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D3-H7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D3-H7) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D3-H7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by 2.5 µg/mL Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D3-H7) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by 2.5 µg/mL Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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869801 | 25 µg | £73 | ||||
869802 | 100 µg | £189 |
Lamin B1 is a type V intermediate filament and an integral component of the nuclear lamina, a meshwork of proteins located inside the nuclear membrane. There are 3 major types of nuclear lamin proteins: lamin A/C, lamin B1 and lamin B2. Lamin A and C are the alternative splicing products of LMNA gene, whereas lamin B1 and lamin B2 are encoded by genes LMNB1 and LMNB2, respectively. Lamins are crucial for maintaining the nuclear structure. They are also important for regulating nuclear functions and activities, including DNA replication and transcription, nuclear and chromatin organization, cell cycle regulation, cell development and differentiation, nuclear migration, and apoptosis. In the brain, lamin B1 levels modulate brain development. Loss of lamin B1 in mouse adversely impairs embryonic brain development and corticogenesis. In addition, duplications of LMNB1 gene causes autosome-dominant leukodystrophy, an adult-onset demyelinating disorder characterized by symmetrical widespread myelin loss in the central nervous system with a phenotype similar to chronic progressive multiple sclerosis.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Lamin B1 protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
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AB_2820180 (BioLegend Cat. No. 869801)
AB_2820181 (BioLegend Cat. No. 869802)
Antigen Details
- Structure
- Human lamin B1 is a 586 amino acid protein with a predicted molecular mass of 66 kD.
- Distribution
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Tissue distribution: Ubiquitously expressed
Cellular distribution: Nucleus - Function
- Lamin B1 is a component of the nuclear lamina, providing a framework for nuclear envelope.
- Interaction
- Lamin B1 interacts with lamin-associated peptides IA, IB, 2, and SPAG4 and SEPT12.
- Cell Type
- Astrocytes, Microglia, Neurons, Oligodendrocytes
- Biology Area
- Cell Biology, Cell Structure, Chromatin Remodeling/Epigenetics, Neurodegeneration, Neuroinflammation, Neuroscience
- Molecular Family
- Nuclear Markers
- Antigen References
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- Padiath QS. et al. 2006. Nat Genet. 38(10):1114-23.
- Camps J. et al. 2015. Nucleus. 6(1):8-14.
- Mahajani S. et al. 2017. Sci Rep. 7(1):4897.
- Gene ID
- 4001 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- Lamin B1
- Specificity Alt (DOES NOT SHOW ON TDS):
- Lamin B1
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,IHC-P
- UniProt
- View information about Lamin B1 on UniProt.org
Related FAQs
Other Formats
View All Lamin B1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Lamin B1 | 5G8-D3-H7 | WB,IHC-P |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Lamin B1
Western blot of purified anti-Lamin B1 antibody (clone 5G8-D... IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D... IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D... IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D... IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D... IHC staining of purified anti-Lamin B1 antibody (clone 5G8-D...
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