- Clone
- 9E10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- myc tag, c-Myc tag, cMyc tag, Myc epitope tag
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/9E10_PURE_c-Myc_Antibody_WB_062618.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysates (15 µg protein) from 293E cells transfected with Multi-tag (lane 1) or non-transfected cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL purified anti-c-Myc antibody (1:500 dilution), clone 9E10 (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-c-Myc antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower blot). Lane M: Molecular weight ladder.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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626807 | 100 µg | £185 | ||||
626808 | 1 mg | £490 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Amino acids 408-439, C-terminal region of human c-myc
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
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WB - Quality tested
ELISA, ICC, IP, IHC-P, Purification - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
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Application References
(PubMed link indicates BioLegend citation) -
- Rao H, et al. 2001. Nature. 410:955.
- Peters CS, et al. 2001. J Biol Chem. 276:13718.
- Munro S, Pelham HR. 1987. Cell. 48:899.
- Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
- Product Citations
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- RRID
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AB_2810685 (BioLegend Cat. No. 626807)
AB_2810686 (BioLegend Cat. No. 626808)
Antigen Details
- Biology Area
- Cell Biology, Protein Purification, Protein Synthesis
- Antigen References
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- Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
- Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
- Battey J, et al. 1983. Cell 34:779.
- Beimling P, et al. 1985. Biochemistry 24:6349.
- Gene ID
- 4609 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- c-Myc
- Specificity Alt (DOES NOT SHOW ON TDS):
- c-Myc
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,ELISA,ICC,IP,IHC-P,Purification
- UniProt
- View information about c-Myc on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All c-Myc Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-c-Myc | 9E10 | WB,ICC,IP,IHC-P,Purification |
Anti-c-Myc Tag (9E10) Affinity Gel | 9E10 | Purification,IP |
Biotin anti-c-Myc | 9E10 | WB,ICC |
Direct-Blot™ HRP anti-c-Myc | 9E10 | WB |
Alexa Fluor® 594 anti-c-Myc | 9E10 | ICC |
Ultra-LEAF™ Purified anti-c-Myc | 9E10 | WB,ELISA,ICC,IP,IHC-P,Purification |
Alexa Fluor® 647 anti-c-Myc | 9E10 | ICC |
Alexa Fluor® 488 anti-c-Myc Antibody | 9E10 | ICC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-c-Myc
Total cell lysates (15 µg protein) from 293E cells transfect... Whole cell extracts (15 µg total protein) from HeLa cells mi... Whole cell extracts (250 µg total protein) prepared from HeL... ICC staining of purified anti-c-Myc antibody (clone 9E10) on... IHC staining of anti-c-Myc antibody (clone 9E10) on formalin... -
Anti-c-Myc Tag (9E10) Affinity Gel
Binding capacity of anti-c-Myc agarose for C-terminal c-Myc ... Stable activity of anti-c-Myc agarose over ten rounds of aff... -
Biotin anti-c-Myc
Western blot analysis of 1, 0.5, 0.1 µl (lanes 1, 2, 3 respe... Immunofluorescence of Myc-tagged plasmid-transfected A549 ce... -
Direct-Blot™ HRP anti-c-Myc
Recombinant Posi-Tag Epitope Tag Protein (1 µL, diluted 1:10... -
Alexa Fluor® 594 anti-c-Myc
HeLa cells transiently transfected with c-Myc tag fused prot... HeLa cells transiently transfected with c-Myc tag fused prot... -
Ultra-LEAF™ Purified anti-c-Myc
Total cell lysates (15 µg protein) from 293E cells transfect... -
Alexa Fluor® 647 anti-c-Myc
Untransfected (panel A) or multi-tag transfected (panel B) H... -
Alexa Fluor® 488 anti-c-Myc Antibody
Untransfected (panel A) or multi-tag transfected (panel B) H...
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