- Clone
- BC96 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V T-072
- Other Names
- Low affinity IL-2R, IL-2R α chain, Tac, p55
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BC96_BV510_CD25_Antibody_080516 BC96_BV510_CD25_Antibody_080516](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BC96_BV510_CD25_Antibody_080516.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) Brilliant Violet 510™ (filled histogram) or mouse IgG1, κ Brilliant Violet 510™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
302639 | 25 tests | £163 | ||||
302640 | 100 tests | £279 |
CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications include: immunocytochemistry3.
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_2629671 (BioLegend Cat. No. 302639)
AB_2629672 (BioLegend Cat. No. 302640)
Antigen Details
- Structure
- Type I transmembrane glycoprotein, 55 kD
- Distribution
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Activated T cells and B cells, monocytes/macrophages, Treg
- Function
- Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681. - Gene ID
- 3559 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD25
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD25
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about CD25 on UniProt.org
Related FAQs
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
FITC anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
PE anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
PE/Cyanine5 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
Purified anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
APC/Cyanine7 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
PE/Cyanine7 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
Alexa Fluor® 488 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
Alexa Fluor® 647 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
Pacific Blue™ anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
Alexa Fluor® 700 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
Biotin anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
PerCP/Cyanine5.5 anti-human CD25
PHA-stimulated (day 3) human peripheral blood lymphocytes we... -
Brilliant Violet 421™ anti-human CD25
Human peripheral blood lymphocytes were stained with CD4 FIT... -
Brilliant Violet 605™ anti-human CD25
Human peripheral blood lymphocytes were stained with CD4 FIT... -
Brilliant Violet 650™ anti-human CD25
Human peripheral blood lymphocytes were stained with CD4 FIT... -
Brilliant Violet 711™ anti-human CD25
Human peripheral blood lymphocytes were stained with CD4 FIT... -
Brilliant Violet 785™ anti-human CD25
Human peripheral blood lymphocytes were stained with CD4 FIT... -
Brilliant Violet 510™ anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
APC/Fire™ 750 anti-human CD25
PHA-stimulated (day 4) human peripheral blood lymphocytes we... -
TotalSeq™-A0085 anti-human CD25
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PE/Dazzle™ 594 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
TotalSeq™-B0085 anti-human CD25
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TotalSeq™-C0085 anti-human CD25
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TotalSeq™-D0085 anti-human CD25
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