- Clone
- L243 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Major Histocompatibility Class II, MHC class II
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![L243_Blue_022008 L243_Blue_022008](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/L243_Blue_022008.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes stained with L243 Pacific Blue™
HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Dog, Common Marmoset, Squirrel Monkey, Cotton-topped Tamarin
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
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test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. - Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The HLA-DR antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per 106 cells in 100 µl volume.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤0.5 µg per 106 cells in 100 µl volume.
It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
-
The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19
Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669). -
Application References
(PubMed link indicates BioLegend citation) -
- Brodsky F. 1984. Immunogenetics 19:179.
- Robbins P, et al. 1987. Human Immunol. 18:301.
- Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161.
- Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC)
- Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC)
- Zipf T, et al. 1981. Cancer Res. 41:4786.
- Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion)
- Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB)
- Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block)
- Wang RF, et al. 1999. Science 284:1351. (Block)
- Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed
- Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
- Stein R, et al. 2011. Leuk. Lymphoma 52:273.
- Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
- Moro M, et al. 2005. BMC Immunol. 6:24.
- Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
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- RRID
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AB_493664 (BioLegend Cat. No. 307623)
AB_493665 (BioLegend Cat. No. 307624)
AB_1595444 (BioLegend Cat. No. 307633)
Antigen Details
- Structure
- Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
- Distribution
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B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
- Function
- Peptide presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138.
3. Edwards JA, et al. 1986. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364. - Gene ID
- 3122 View all products for this Gene ID 3123 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- HLA-DR
- Specificity Alt (DOES NOT SHOW ON TDS):
- HLA-DR
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about HLA-DR on UniProt.org
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 APC -
FITC anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 FITC -
PE anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PE -
PE/Cyanine5 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PE/Cyan... -
Purified anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 AP... -
Biotin anti-human HLA-DR
Human peripheral blood lymphocytes stained with biotinylated... -
PE/Cyanine7 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PE/Cyan... -
APC/Cyanine7 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
Alexa Fluor® 488 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Alexa F... Confocal image of human lymph node sample acquired using the... Confocal image of human lymph node sample acquired using the... Confocal image of human liver sample acquired using the IBEX... -
Alexa Fluor® 647 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Alexa F... -
Pacific Blue™ anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Pacific... -
Alexa Fluor® 700 anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 Alexa F... -
PerCP anti-human HLA-DR
Human peripheral blood lymphocytes stained with L243 PerCP -
PerCP/Cyanine5.5 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 605™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 421™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 570™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 711™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 785™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Brilliant Violet 510™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
Ultra-LEAF™ Purified anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 650™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
Purified anti-human HLA-DR (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 174Yb-an... -
PE/Dazzle™ 594 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with HLA-DR ... -
FITC anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 PE... -
Pacific Blue™ anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
PE/Dazzle™ 594 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-A0159 anti-human HLA-DR
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TotalSeq™-B0159 anti-human HLA-DR
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TotalSeq™-C0159 anti-human HLA-DR
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Brilliant Violet 750™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
APC/Fire™ 750 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes and ... -
PerCP/Cyanine5.5 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 810 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
PE/Fire™ 640 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with CD19 FI... -
PE anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 538 anti-human HLA-DR Antibody
Human peripheral blood lymphocytes were stained with CD19 AP... -
KIRAVIA Blue 520™ anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0159 anti-human HLA-DR
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PE/Fire™ 810 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE/Dazzle™ 594 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 423 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP FITC anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP APC anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP PE/Cyanine7 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP Pacific Blue™ anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP APC/Fire™ 750 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes and ... -
Spark Violet™ 500 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PerCP/Cyanine5.5 anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
GMP PE anti-human HLA-DR
Typical results from human peripheral blood lymphocytes stai... -
Spark UV™ 387 anti-human HLA-DR
Human peripheral blood lymphocytes stained with anti-human C... -
Spark Blue™ 515 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark NIR™ 685 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 700 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Blue™ 550 anti-human HLA-DR (Flexi-Fluor™)
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Spark Red™ 718 anti-human HLA-DR (Flexi-Fluor™)
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PE/Fire™ 744 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark PLUS UV™ 395 anti-human HLA-DR
Human peripheral blood lymphocytes were stained with anti-hu...
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