- Clone
- 6E10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
- Previously
-
Signet Catalog# 9320-02
Signet Catalog# 9320-05
Signet Catalog# 9320-10
Covance Catalog# SIG-39320
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_6E10_PURE_betaAmyloid_1-16_WB_050818 A_6E10_PURE_betaAmyloid_1-16_WB_050818](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/6E10_PURE_betaAmyloid_1-16_WB_050818.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Western blot of purified anti-β-amyloid, 1-16 antibody (clone 6E10). Lane 1: Molecular weight marker; Lane 2: 50 ng of the human Aβ1-40 peptide; Lane 3: 50 ng of the Aβ1-42 peptide; Lane 4: 50 ng of the recombinant human APP751 protein; Lane 5: 20µg of the human brain lysate; Lane 6: 50 ng of the rodent Aβ1-42 peptide. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with the HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
Direct ELISA of purified anti-β-amyloid 1-16 (clone 6E10) antibody binding to the plate-immobilized human Aβ1-40 and the recombinant human APP751. ELISA was performed by coating the wells with 100 ng of peptide or recombinant protein. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with HRP labeled goat anti-mouse IgG secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system. -
IHC staining of purified anti-β-Amyloid, 1-16 antibody (clone 6E10) on the formalin-fixed paraffin-embedded normal human (left panel) and Alzheimer’s disease (right panel) brain tissues. Following antigen retrieval using 70% formic acid for 20 minutes, the tissues were incubated with 1µg/mL of the primary antibody for 1 hour at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution (no preservatives or carrier proteins).
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
Direct ELISA, IHC-P - Verified
IHC-F, EM, ICC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1 µg/mL. For Direct ELISA, the suggested use of this reagent is 0.002 - 0.02 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 0.2 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is reactive to amino acid residue 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS).
This antibody clone has been reported for use in immunohistochemistry of free-floating sections2,13. - Additional Product Notes
-
View more applications data for this product in our Scientific Poster Library.
-
Application References
(PubMed link indicates BioLegend citation) -
- Thakker DR, et al. 2009. Proc. Natl. Acad. Sci. USA. 106(11):4501-6. (IHC) PubMed
- Oddo S, et al. 2005. Proc. Natl. Acad. Sci. USA. 102(8):3046-51. (IHC-other) PubMed
- Herzig M, et al. 2004. Nat. Neuro. 7(9):954-959. (WB) PubMed
- Zheng Y, et al. 2012. PLoS One 6:39035. (IHC-F) PubMed
- Abramowksi D, et al. J Neurosci. 32:1273. (WB) PubMed
- Forny-Germano L, et al. 2014. J. Neurosci. 34:13629. (WB, IHC) PubMed
- Gowert NS, et al. 2014. PLoS One 2:e90523. (ICC, EM) PubMed
- Sandoval-Hernández A, et al. 2015. PLoS One. 10: 0145467. (IHC-F)
- Kumar R, et al. 2016. Brain. 139:174-92 (WB)
- Miyamoto T, et al. 2016. J. Biol. Chem. 291:1719-34. (WB)
- Saito S, et al. 2017. Acta Neuropathol. Commun. 5:26-9. (IHC-P) PubMed
- Omata Y, et al. 2016. Aging (Albany NY) 8(3):427. (IHC-P) PubMed
- Peng W, et al. 2016. Neurobiol. Dis. 93:215. (IHC-other) PubMed
- Mandler M, et al. 2015. PLoS One. e0115237. (WB, IHC, ELISA) PubMed
- Product Citations
-
- RRID
-
AB_2715854 (BioLegend Cat. No. 803004)
AB_2564653 (BioLegend Cat. No. 803001)
AB_2564654 (BioLegend Cat. No. 803002)
AB_2564652 (BioLegend Cat. No. 803003)
Antigen Details
- Structure
- Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
- Distribution
-
Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus. - Function
- The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- APP/β-Amyloid
- Antigen References
-
- Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195.
- Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1
- Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83.
- Puig KL, et al. 2012. Exp. Gerontol. 48(7): 608.
- Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595.
- Walsh DM, et al. 2007. J. Neurochem. 101(5):1172.
- Gene ID
- 351 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- beta-Amyloid 1-16
- Specificity Alt (DOES NOT SHOW ON TDS):
- β-Amyloid, 1-16
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,Direct ELISA,IHC-P,IHC-F,EM,ICC
- UniProt
- View information about beta-Amyloid 1-16 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All β-Amyloid, 1-16 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Alexa Fluor® 488 anti-β-Amyloid, 1-16 | 6E10 | IHC-P |
Anti-β-Amyloid, 1-16 | 6E10 | WB,Direct ELISA,IHC-P,IHC-F,EM |
Biotin anti-β-Amyloid, 1-16 | 6E10 | IHC-P |
Purified anti-β-Amyloid, 1-16 | 6E10 | WB,Direct ELISA,IHC-P,IHC-F,EM,ICC |
HRP anti-β-Amyloid, 1-16 | 6E10 | IHC-P,WB |
Alexa Fluor® 594 anti-β-Amyloid, 1-16 | 6E10 | IHC-P |
Alexa Fluor® 647 anti-β-Amyloid, 1-16 | 6E10 | IHC-P |
Ultra-LEAF™ Purified anti-β-Amyloid, 1-16 | 6E10 | WB,IHC-P,ICC,IHC-F,EM |
Spark YG™ 570 anti-β-Amyloid, 1-16 | 6E10 | IHC-P |
Customers Also Purchased
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/4G8_Ascites_Beta-Amyloid_Antibody_1_091118.png)
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/LN27_PURE_APP_WB_110918.png)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Alexa Fluor® 488 anti-β-Amyloid, 1-16
IHC staining of Alexa Fluor® 488 anti-β amyloid, 1-16 antibo... -
Anti-β-Amyloid, 1-16
Western blot of anti-β-amyloid, 1-16 antibody (clone 6E10). ... Direct ELISA of anti-β-amyloid, 1-16 (clone 6E10) antibody b... IHC staining of anti-β-Amyloid, 1-16 antibody (clone 6E10) o... -
Biotin anti-β-Amyloid, 1-16
IHC staining of biotin anti-β-Amyloid, 1-16 antibody (clone ... -
Purified anti-β-Amyloid, 1-16
Western blot of purified anti-β-amyloid, 1-16 antibody (clon... Direct ELISA of purified anti-β-amyloid 1-16 (clone 6E10) an... IHC staining of purified anti-β-Amyloid, 1-16 antibody (clo... -
HRP anti-β-Amyloid, 1-16
IHC staining of HRP anti-β-Amyloid, 1-16 antibody (clone 6E1... Western blot of HRP anti-β-Amyloid, 1-16 antibody (clone 6E1... -
Alexa Fluor® 594 anti-β-Amyloid, 1-16
IHC staining of Alexa Fluor® 594 anti-β amyloid, 1-16 antibo... -
Alexa Fluor® 647 anti-β-Amyloid, 1-16
IHC staining of Alexa Fluor® 647 anti-β-Amyloid, 1-16 antibo... IHC staining of Alexa Fluor® 647 anti-β-Amyloid, 1-16 antibo... -
Ultra-LEAF™ Purified anti-β-Amyloid, 1-16
Western blot of Ultra-LEAF™ Purified anti-β-Amyloid, 1-16 an... IHC staining of Ultra-LEAF™ Purified anti-β-Amyloid, 1-16 an... IHC staining of Ultra-LEAF™ Purified anti-β-Amyloid, 1-16 an... -
Spark YG™ 570 anti-β-Amyloid, 1-16
IHC staining of Spark YG™ 570 anti-β-Amyloid, 1-16 antibody ...
Follow Us