- Clone
- 4B10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T-box expressed in T cells, T box 21, TBLYM
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![4B10_Purified_TBet_Cytof_061114.jpg 4B10_Purified_TBet_Cytof_061114.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/4B10_Purified_TBet_Cytof_061114.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human PBMCs were fixed, permeabilized, and stained with 154Sm-anti-CD3 (UCHT1) and 160Gd-anti-T-bet (4B10). Data provided by DVS Sciences.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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644825 | 100 µg | £165 |
T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
CyTOF® - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.
NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601). - Additional Product Notes
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Maxpar® is a registered trademark of Standard BioTools Inc.
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Application References
(PubMed link indicates BioLegend citation) -
- Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
- Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
- Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
- Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
- Product Citations
-
- RRID
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AB_2563788 (BioLegend Cat. No. 644825)
Antigen Details
- Structure
- T-box transcription factor, approximately 58 kD.
- Distribution
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Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
- Function
- Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
- Cell Type
- B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
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1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907. - Gene ID
- 30009 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- T-bet
- Specificity Alt (DOES NOT SHOW ON TDS):
- T-bet
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,CyTOF®
- UniProt
- View information about T-bet on UniProt.org
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
-
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
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We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
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The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All T-bet Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
APC anti-T-bet | 4B10 | ICFC |
Purified anti-T-bet | 4B10 | WB,ICFC,IHC-F,IP |
Alexa Fluor® 647 anti-T-bet | 4B10 | ICFC |
PerCP/Cyanine5.5 anti-T-bet | 4B10 | ICFC |
Pacific Blue™ anti-T-bet | 4B10 | ICFC |
PE anti-T-bet | 4B10 | ICFC |
Brilliant Violet 711™ anti-T-bet | 4B10 | ICFC |
FITC anti-T-bet | 4B10 | ICFC |
Brilliant Violet 421™ anti-T-bet | 4B10 | ICFC |
Brilliant Violet 605™ anti-T-bet | 4B10 | ICFC |
PE/Cyanine7 anti-T-bet | 4B10 | ICFC |
PE/Dazzle™ 594 anti-T-bet | 4B10 | ICFC |
Purified anti-T-bet (Maxpar® Ready) | 4B10 | WB,CyTOF® |
Alexa Fluor® 488 anti-T-bet | 4B10 | ICFC |
Brilliant Violet 785™ anti-T-bet | 4B10 | ICFC |
Alexa Fluor® 594 anti-T-bet | 4B10 | ICFC,ICC |
KIRAVIA Blue 520™ anti-T-bet | 4B10 | ICFC |
PE/Fire™ 810 anti-T-bet | 4B10 | ICFC |
PE/Cyanine5 anti-T-bet | 4B10 | ICFC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Purified anti-T-bet
Total cell lysate from PBMC (lane 1, 15 µg) and PBMC treated... Human peripheral blood lymphocytes were surface stained with... Total lysates (15µg protein) from Daudi, K562 and NK-92 cell... -
Alexa Fluor® 647 anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
PerCP/Cyanine5.5 anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Pacific Blue™ anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
PE anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 711™ anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
FITC anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 421™ anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 605™ anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
PE/Cyanine7 anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
PE/Dazzle™ 594 anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Purified anti-T-bet (Maxpar® Ready)
Human PBMCs were fixed, permeabilized, and stained with 154S... -
Alexa Fluor® 488 anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 785™ anti-T-bet
Human peripheral blood lymphocytes were stained with CD3 APC... -
Alexa Fluor® 594 anti-T-bet
NK-92 cells were fixed with 4% paraformaldehyde (PFA) for 15... NK-92 cells were fixed with 4% paraformaldehyde (PFA) for 15... PBMCs were surface stained with Alexa Fluor® 488 anti-CD3 (C... -
KIRAVIA Blue 520™ anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
PE/Fire™ 810 anti-T-bet
Human peripheral blood lymphocytes were surface stained with... -
PE/Cyanine5 anti-T-bet
Human peripheral blood mononuclear cells were surfaced stain...
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