- Clone
- N28/9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SLC17A7, Vesicular glutamate transporter 1, brain-specific Na-dependent inorganic phosphate cotransporter, brain-specific Na(+)-dependent inorganic phosphate cotransporter, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter)
- Previously
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Covance Catalog# MMS-5245
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_N28slash9_PURE_VGlut1_WB_110118 1_N28slash9_PURE_VGlut1_WB_110118](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/N28slash9_PURE_VGlut1_WB_110118.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-VGlut1 antibody (clone N28/9). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-VGlut1 antibody (clone N28/9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. -
IHC staining of purified anti-VGlut1 antibody (clone N28/9) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-VGlut1 antibody (clone N28/9) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
821302 | 25 µL | £57 | ||||
821301 | 100 µL | £113 |
Vesicular glutamate transporter (VGlut) 1, also known as solute carrier family 17 member 7 (SLC17A7) and brain-specific sodium-dependent inorganic phosphate cotransporter, is a vesicle-bound transporter of glutamate that acts in a sodium-dependent manner. It located within the membranes of synaptic vesicles and is specifically expressed in neurons.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).
- Product Citations
-
- RRID
-
AB_2734585 (BioLegend Cat. No. 821302)
AB_2564849 (BioLegend Cat. No. 821301)
Antigen Details
- Structure
- Expected MW: 52 kD
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Presynaptic proteins
- Gene ID
- 116638 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- VGlut1
- Specificity Alt (DOES NOT SHOW ON TDS):
- VGlut1
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,IHC-P
- UniProt
- View information about VGlut1 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All VGlut1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-VGlut1 | N28/9 | WB,IHC-P |
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