- Clone
- MRM-47 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin M
- Isotype
- Mouse IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MRM-47_A647_IgM_Antibody_1_062819 MRM-47_A647_IgM_Antibody_1_062819](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MRM-47_A647_IgM_Antibody_1_062819.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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LEW/s rat splenocytes were stained with rat IgM(clone MRM-47) Alexa Fluor® 488(filled histogram), or rat IgM isotype control(open histogram). -
C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of Purified PNAd (clone MECA-79) at 4°C overnight. The staining was followed by 2.5 µg/ml anti-rat IgM (MRM-47) Alexa Fluor® 488 (green) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 594(red) at room temperature for 2 hours. The image was captured by 10X objective. -
C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of Purified PNAd (clone MECA-79) at 4°C overnight. The staining was followed by 2.5 µg/ml anti-rat IgM (MRM-47) Alexa Fluor® 488 (green) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 594(red) at room temperature for 2 hours. The image was captured by 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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408907 | 25 µg | £70 | ||||
408908 | 100 µg | £157 |
The MRM-47 monoclonal antibody reacts with rat immunoglobulin M (IgM) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRM-47 antibody can be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mixed rat Igs
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
IHC-F - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Product Citations
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- RRID
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AB_2814389 (BioLegend Cat. No. 408907)
AB_2814390 (BioLegend Cat. No. 408908)
Other Formats
View All IgM Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-rat IgM | MRM-47 | FC,ELISA |
Biotin anti-rat IgM | MRM-47 | FC,ELISA,IHC-F |
FITC anti-rat IgM | MRM-47 | FC |
Alexa Fluor® 594 anti-rat IgM | MRM-47 | IHC-F |
Alexa Fluor® 647 anti-rat IgM | MRM-47 | FC,IHC-F |
Alexa Fluor® 488 anti-rat IgM | MRM-47 | FC,IHC-F |
PE/Cyanine7 anti-rat IgM | MRM-47 | FC |
PerCP/Cyanine5.5 anti-rat IgM | MRM-47 | FC |
PE anti-rat IgM | MRM-47 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-rat IgM
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Biotin anti-rat IgM
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
FITC anti-rat IgM
LOU rat splenocytes stained with MRM-47 FITC and MHC class I... -
Alexa Fluor® 594 anti-rat IgM
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
Alexa Fluor® 647 anti-rat IgM
Lewis rat splenocytes were stained with rat IgM(clone MRM-47... C57BL/6 mouse frozen lymph node section was fixed with 4% pa... -
Alexa Fluor® 488 anti-rat IgM
LEW/s rat splenocytes were stained with rat IgM(clone MRM-47... C57BL/6 mouse frozen lymph node section was fixed with 4% pa... C57BL/6 mouse frozen lymph node section was fixed with 4% pa... -
PE/Cyanine7 anti-rat IgM
Rat splenocytes were stained with rat IgM (clone MRM-47) PE/... -
PerCP/Cyanine5.5 anti-rat IgM
Rat splenocytes were stained with rat IgM (clone MRM-47) Per... -
PE anti-rat IgM
LOU rat splenocytes were stained with anti-rat CD45RA APC an...
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