- Clone
- AY13 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, HER1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_AY13_AF594_EGFR_IF_032318 A_AY13_AF594_EGFR_IF_032318](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/AY13_AF594_EGFR_IF_032318.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
HeLa cells were fixed with 1% paraformaldehyde (PFA) and blocked with 5% FBS for 30 minutes. Then the cells were stained with 10 µg/ml anti-human EGFR (clone AY13) Alexa Fluor® 594 (red) at room temperature for 3 hours in dark. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective. -
Formalin-fixed paraffin-embedded human breast cancer tissue slices were deparaffinized and rehydrated. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes, washed with PBS/ 0.05% Tween20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the slices were stained with 10 µg/ml of Alexa Fluor® 594 anti-human EGFR (clone AY13) (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
352916 | 100 µg | £230 |
Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein and member of the protein kinase superfamily that regulates cell growth and differentiation. EGFR binds EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor - all members of the EGF family. Ligand binding induces EGFR dimerization and autophosphorylation, initiating the MAPK, Akt, and JNK signaling pathways. EGFR is expressed by epithelial and endothelial cells and is frequently expressed by epithelial carcinomas.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Non-small cell lung cancer (NSCLC) cell line NCI-H322
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 10 μg/mL is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Yamaguchi M, et al. 2009. The 15th Annual Meeting Japan Society of Gene Therapy. p1056. Abstract 92.
- RRID
-
AB_2563210 (BioLegend Cat. No. 352916)
Antigen Details
- Structure
- Member of the protein kinase superfamily; transmembrane glycoprotein; ligand binding induces dimerization and autophosphorylation
- Distribution
-
Epithelial cells and endothelial cells; frequently found in epithelial carcinomas
- Function
- Controls cell growth and differentiation
- Interaction
- MAPK, Akt, JNK
- Ligand/Receptor
- Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor
- Cell Type
- Endothelial cells, Epithelial cells
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
- Molecular Family
- CD Molecules, Growth Factors
- Antigen References
-
1. da Cunha Santos G, et al. 2011. Annu. Rev. Pathol. 6:49.
2. Gusterson BA and Hunter KD. 2009. Lancet Oncol. 10:522.
3. Mano M and Humblet Y. 2008. Nat. Clin. Pract. Oncol. 5:415.
4. Pao W and Chmielecki J. 2010. Nat. Rev. Cancer 10:760. - Gene ID
- 1956 View all products for this Gene ID
- UniProt
- View information about EGFR on UniProt.org
Other Formats
View All EGFR Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human EGFR | AY13 | FC,ICC |
PE anti-human EGFR | AY13 | FC |
APC anti-human EGFR | AY13 | FC |
Alexa Fluor® 488 anti-human EGFR | AY13 | FC,ICC |
Brilliant Violet 421™ anti-human EGFR | AY13 | FC,ICC |
PE/Cyanine7 anti-human EGFR | AY13 | FC |
PerCP/Cyanine5.5 anti-human EGFR | AY13 | FC |
Alexa Fluor® 594 anti-human EGFR | AY13 | ICC,IHC-P |
Alexa Fluor® 647 anti-human EGFR | AY13 | FC |
Brilliant Violet 711™ anti-human EGFR | AY13 | FC |
PE/Dazzle™ 594 anti-human EGFR | AY13 | FC |
TotalSeq™-A0132 anti-human EGFR | AY13 | PG |
Brilliant Violet 605™ anti-human EGFR | AY13 | FC |
APC/Fire™ 750 anti-human EGFR | AY13 | FC |
TotalSeq™-B0132 anti-human EGFR | AY13 | PG |
TotalSeq™-C0132 anti-human EGFR | AY13 | PG |
Biotin anti-human EGFR | AY13 | FC |
TotalSeq™-D0132 anti-human EGFR | AY13 | PG |
Brilliant Violet 510™ anti-human EGFR | AY13 | FC |
Brilliant Violet 650™ anti-human EGFR | AY13 | FC |
Brilliant Violet 785™ anti-human EGFR | AY13 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human EGFR
HELA cells stained with purified EGFR (clone AY13, filled hi... HeLa cells were fixed with 2% PFA for ten minutes, permeabil... -
PE anti-human EGFR
Human cervical cancer cell line HELA was stained with EGFR (... -
APC anti-human EGFR
Human cervical cancer cell line HELA was stained with EGFR (... -
Alexa Fluor® 488 anti-human EGFR
Human cervical cancer cell line HeLa was stained with EGFR (... HeLa cells were fixed with 1% paraformaldehyde (PFA) and the... -
Brilliant Violet 421™ anti-human EGFR
Human cervical cancer cell line Hela was stained with EGFR (... HeLa cells were fixed with 1% paraformaldehyde solution and ... -
PE/Cyanine7 anti-human EGFR
Human cervical cancer cell line, Hela, was stained with EGFR... -
PerCP/Cyanine5.5 anti-human EGFR
HeLa cells were stained with EGFR (clone AY13) PerCP/Cyanine... -
Alexa Fluor® 594 anti-human EGFR
HeLa cells were fixed with 1% paraformaldehyde (PFA) and blo... Formalin-fixed paraffin-embedded human breast cancer tissue ... -
Alexa Fluor® 647 anti-human EGFR
Human cervical cancer cell line HELA was stained with EGFR (... -
Brilliant Violet 711™ anti-human EGFR
Human cervical cancer cell line HELA was stained with EGFR (... -
PE/Dazzle™ 594 anti-human EGFR
Human cervical cancer cell line HELA was stained with EGFR (... -
TotalSeq™-A0132 anti-human EGFR
-
Brilliant Violet 605™ anti-human EGFR
Human cervical cancer cell line HELA was stained with EGFR (... -
APC/Fire™ 750 anti-human EGFR
Human cervical cancer cell line HELA was stained with EGFR (... -
TotalSeq™-B0132 anti-human EGFR
-
TotalSeq™-C0132 anti-human EGFR
-
Biotin anti-human EGFR
Human cervical cancer cell line HELA was stained with biotin... -
TotalSeq™-D0132 anti-human EGFR
-
Brilliant Violet 510™ anti-human EGFR
Human cervical cancer cell line HeLa was stained with anti-h... -
Brilliant Violet 650™ anti-human EGFR
Human cervical cancer cell line HeLa was stained with anti-h... -
Brilliant Violet 785™ anti-human EGFR
Human cervical cancer cell line HeLa was stained with anti-h...
Follow Us