- Clone
- OX-97 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Lyb-8, BL-CAM
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![OX-97_A647_081908.jpg OX-97_A647_081908.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/OX-97_A647_081908.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes stained with OX-97 Alexa Fluor® 647 -
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of CD22 (clone OX-97) Alexa Fluor® 647 (red) and CD3 (clone 17A2) Alexa Fluor® 594 (green) overnight at 4°C. The image was captured by 10X objective. -
Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse CD3ε Antibody (clone 500A2) Alexa Fluor® 594 at 5 µg/mL (green), and anti-mouse CD22 Antibody (clone OX-97) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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Cat # | Size | Price | Quantity Check Availability | Save | ||
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126107 | 25 µg | £85 | ||||
126108 | 100 µg | £182 |
CD22 is a member of the sialoadhesion family.CD22 is expressed on the surface of a subpopulation of mature B lymphocytes and may modulate signaling through the B cell antigen receptor. The OX-97 monoclonal antibody recognizes the second domain of the CD22.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F, 3D IHC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemistry, a concentration range of 2.5 - 5 µg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Product Citations
-
- RRID
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AB_2074574 (BioLegend Cat. No. 126107)
AB_2074573 (BioLegend Cat. No. 126108)
Antigen Details
- Structure
- 130-150 kd type 1 transmembrane glycoprotein. It is a member of the Siglec family and Ig superfamily.
- Distribution
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CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells.
- Function
- B cell signaling and adhesion.
- Interaction
- Associates with SHP-1, Syk, Lck, Lyn and others.
- Cell Type
- B cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Symington FW et al. 1982. Immunogenetics 16:381
2. Erickson LD et al. 1996. Int Immunol 8:1121
3. Doody GM et al. 1995. Science 269:242 - Gene ID
- 12483 View all products for this Gene ID
- UniProt
- View information about CD22 on UniProt.org
Other Formats
View All CD22 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-mouse CD22 | OX-97 | FC |
Alexa Fluor® 647 anti-mouse CD22 | OX-97 | FC,IHC-F,3D IHC |
APC anti-mouse CD22 | OX-97 | FC |
PE anti-mouse CD22 | OX-97 | FC |
TotalSeq™-A0827 anti-mouse CD22 | OX-97 | PG |
TotalSeq™-C0827 anti-mouse CD22 | OX-97 | PG |
TotalSeq™-B0827 anti-mouse CD22 | OX-97 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse CD22
C57BL/6 splenocytes stained with OX-97 FITC -
Alexa Fluor® 647 anti-mouse CD22
C57BL/6 splenocytes stained with OX-97 Alexa Fluor® 647 C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
APC anti-mouse CD22
C57BL/6 mouse splenocytes were stained with CD22 (clone OX-9... -
PE anti-mouse CD22
C57BL/6 mouse splenocytes were stained with CD22 (clone OX-9... -
TotalSeq™-A0827 anti-mouse CD22
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TotalSeq™-C0827 anti-mouse CD22
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TotalSeq™-B0827 anti-mouse CD22
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