- Clone
- RMK-45 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin light chain kappa
- Isotype
- Rat IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RMK-45_AF700_IgK_Antibody_FC_061714 RMK-45_AF700_IgK_Antibody_FC_061714](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RMK-45_AF700_IgK_Antibody_FC_061714.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes were stained with anti-mouse Ig light chain κ (clone RMK-45) Alexa Fluor® 700 (filled histogram) or rat IgG1, κ Alexa Fluor® 700 isotype control (open histogram). -
Human peripheral blood lymphocytes were stained with (filled histogram) or without (open histogram) purified mouse IgG1, κ ant-human CD3 (clone UCHT1), followed by anti-mouse Ig light chain κ (clone RMK-45) Alexa Fluor® 700.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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409507 | 25 µg | £85 | ||||
409508 | 100 µg | £221 |
The RMK-45 monoclonal antibody reacts with immunoglobulin light chain kappa in all tested mouse haplotype (Igh-a and b). It does not react with the lambda chain.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
The RMK-12 monoclonal antibody may be used as a primary antibody for ELISA or as secondary for detection of mouse Ig kappa.
- Product Citations
-
- RRID
-
AB_2563582 (BioLegend Cat. No. 409507)
AB_2563583 (BioLegend Cat. No. 409508)
Antigen Details
- Structure
- Immunoglobulin family
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Synthesis
- Antigen References
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1. Ludwig TE, et al. 2006. Nat. Methods. 3:637.
2. Nguyen DH, et al. 2005. J. Immunol. 175:228.
3. Bardor M, et al. 2005. J. Biol. Chem. 280:4228.
4. Diaz SL, et al. 2009. PLoS One. 4:e4241. - Gene ID
- 243469 View all products for this Gene ID
- UniProt
- View information about Ig light chain kappa on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Ig light chain κ Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse Ig light chain κ | RMK-45 | FC,ELISA |
APC/Cyanine7 anti-mouse Ig light chain κ | RMK-45 | FC |
PE anti-mouse Ig light chain κ | RMK-45 | FC |
Alexa Fluor® 700 anti-mouse Ig light chain κ | RMK-45 | FC |
FITC anti-mouse Ig light chain κ | RMK-45 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse Ig light chain κ
C57BL/6 splenocytes were stained with purified anti-mouse li... Human peripheral blood lymphocytes were stained with purifie... -
APC/Cyanine7 anti-mouse Ig light chain κ
C57BL/6 splenocytes were stained with anti-mouse Ig light ch... Human peripheral blood lymphocytes were stained with (filled... -
PE anti-mouse Ig light chain κ
C57BL/6 splenocytes were stained with anti-mouse Ig light ch... Human peripheral blood lymphocytes were stained with (filled... -
Alexa Fluor® 700 anti-mouse Ig light chain κ
C57BL/6 splenocytes were stained with anti-mouse Ig light ch... Human peripheral blood lymphocytes were stained with (filled... -
FITC anti-mouse Ig light chain κ
C57BL/6 splenocytes were stained with anti-mouse Ig light ch... Human peripheral blood lymphocytes were stained with (filled...
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