- Clone
- I3/2.3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T200, Ly-5, LCA
- Isotype
- Rat IgG2b, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![I32.3_APC_CD45_Antibody_FC_050714.jpg I32.3_APC_CD45_Antibody_FC_050714.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/I32.3_APC_CD45_Antibody_FC_050714.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes were stained with CD45 (clone I3/2.3) APC (filled histogram) or rat IgG2b APC isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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147707 | 25 µg | £54 | ||||
147708 | 100 µg | £151 |
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from alternative splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse lymphoma cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin embedded sections1 and frozen tissue sections2.
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Application References
(PubMed link indicates BioLegend citation) -
- Kliment C, et al. 2009. J. Mol. Cell Cardiol. 47:730. (IHC)
- Reynolds JM, et al. 2007. J. Immunol. 179:313. (IHC)
- Product Citations
-
- RRID
-
AB_2563539 (BioLegend Cat. No. 147707)
AB_2563540 (BioLegend Cat. No. 147708)
Antigen Details
- Structure
- Protein tyrosine phosphatase (PTP) family, 180-240 kD
- Distribution
-
All hematopoietic cells except mature erythrocytes and platelets
- Function
- Phosphatase, T and B cell activation
- Ligand/Receptor
- Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
- Cell Type
- B cells, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS and Thomas ML. 1994. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851. - Gene ID
- 19264 View all products for this Gene ID
- UniProt
- View information about CD45 on UniProt.org
Related FAQs
Other Formats
View All CD45 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD45 | I3/2.3 | FC,IHC-P,IHC-F,SB |
PerCP/Cyanine5.5 anti-mouse CD45 | I3/2.3 | FC |
PE/Cyanine7 anti-mouse CD45 | I3/2.3 | FC |
APC anti-mouse CD45 | I3/2.3 | FC |
FITC anti-mouse CD45 | I3/2.3 | FC |
PE anti-mouse CD45 | I3/2.3 | FC |
APC/Fire™ 750 anti-mouse CD45 | I3/2.3 | FC |
Alexa Fluor® 700 anti-mouse CD45 | I3/2.3 | FC |
APC/Cyanine7 anti-mouse CD45 Antibody | I3/2.3 | FC |
Brilliant Violet 421™ anti-mouse CD45 | I3/2.3 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD45
C57BL/6 splenocytes were stained with purified CD45 (clone I... -
PerCP/Cyanine5.5 anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone I3/2.3) Pe... -
PE/Cyanine7 anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone I3/2.3) PE... -
APC anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone I3/2.3) AP... -
FITC anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone I3/2.3) FI... -
PE anti-mouse CD45
C57BL/6 splenocytes were stained with CD45 (clone I3/2.3) PE... -
APC/Fire™ 750 anti-mouse CD45
C57BL/6 mouse splenocytes were stained with CD45 (clone I3/2... -
Alexa Fluor® 700 anti-mouse CD45
C57BL/6 mouse splenocytes were stained with CD45 (clone I3/2... -
APC/Cyanine7 anti-mouse CD45 Antibody
C57BL/6 splenocytes were stained with anti-mouse CD45 (clone... -
Brilliant Violet 421™ anti-mouse CD45
C57BL/6 mouse splenocytes were stained with anti-mouse CD3 (... C57BL/6 mouse splenocytes were stained with anti-mouse CD45 ...
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