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APC/Cyanine7 anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_APCCy7_022309.jpg
Mouse thymic epithelial stromal cell line TE-71 stained with G8.8 APC/Cyanine7
  • G8dot8_APCCy7_022309.jpg
    Mouse thymic epithelial stromal cell line TE-71 stained with G8.8 APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
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118217 25 µg £101
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118218 100 µg £238
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Zwarycz B, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:1. PubMed
  2. Ochiai S, et al. 2014. J Immunol. 193:2504. PubMed
  3. Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed
  4. Sefik E, et al. 2022. Nature. 606:585. PubMed
  5. Lorenzo-Martín LF, et al. 2022. Oncogene. 41:3341. PubMed
  6. Krishnamurty AT, et al. 2022. Nature. 611:148. PubMed
  7. Setia M, et al. 2023. STAR Protoc. 4:102056. PubMed
  8. Jowett GM, et al. 2022. Cell Rep. 40:111281. PubMed
  9. Weeden C, et al. 2017. PLoS Biol. 10.1371/journal.pbio.2000731. PubMed
  10. Karlsson J, et al. 2021. Adv Funct Mater. 31:. PubMed
  11. Ruth JR, et al. 2021. Breast Cancer Res. 23:63. PubMed
  12. Dumont-Lagacé M, et al. 2014. J Immunol. 192:2219. PubMed
  13. Moreau HD et al. 2019. Dev Cell. 49(2):171-188 . PubMed
  14. Zaid A, et al. 2014. Proc Natl Acad Sci U S A. 111:5307. PubMed
  15. Cen B, et al. 2021. Oncogene. 40:5984. PubMed
  16. Xu Q, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  17. Agarwal S, et al. 2015. Cell Rep. 13: 2147-2158. PubMed
  18. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  19. Romera–Hernández M, et al. 2020. Cell Reports. 30(1):37-45.e3.. PubMed
  20. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  21. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  22. Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed
  23. Kim M, et al. 2015. J Immunol. 194:4748. PubMed
  24. Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed
  25. Guo Q, et al. 2022. Nat Commun. 13:3837. PubMed
  26. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  27. Morral C, et al. 2020. Cell Stem Cell. 26(6):845-861. PubMed
  28. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  29. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  30. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  31. Sitaraman S, et al. 2019. Sci Rep. 9:12509. PubMed
  32. Lee S et al. 2017. Cell host & microbe. 22(4):449-459 . PubMed
  33. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  34. Wells KL, et al. 2020. Elife. 9:. PubMed
  35. Mulholland D, et al. 2012. Cancer Res. 72:1878. PubMed
  36. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  37. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  38. Manzo N, et al. 2012. Am J Respir Cell Mol Biol. 47:349. PubMed
  39. Douchi D, et al. 2022. Cell Mol Gastroenterol Hepatol. 13:1317. PubMed
  40. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  41. St-Pierre C, et al. 2015. J Immunol. 195: 498 - 506. PubMed
  42. El Agha E, et al. 2017. Cell Stem Cell. 1.014583333. PubMed
  43. Di Campli MP, et al. 2020. Eur Respir J. . PubMed
  44. Ikonomou L, et al. 2020. Nat Commun. 0.899305556. PubMed
  45. Pascual R, et al. 2020. Sci Adv. 6:eaax3868. PubMed
  46. Deliyannis G, et al. 2021. JCI Insight. 6:. PubMed
  47. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  48. O'Reilly LA, et al. 2018. Immunity. 48:570. PubMed
  49. Dumont-Lagacé M, et al. 2015. Sci Rep. 5: 12895. PubMed
  50. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  51. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  52. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
  53. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  54. Garibaldi B, et al. 2013. Am J Respir Cell Mol Biol. 48:35. PubMed
  55. Sfakianos JP, et al. 2020. Nat Commun. 2.222222222. PubMed
  56. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  57. Bretou M, et al. 2017. Sci Immunol. 2:16. PubMed
RRID
AB_1501158 (BioLegend Cat. No. 118217)
AB_2098648 (BioLegend Cat. No. 118218)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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