- Clone
- S17016D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NKR-P1C, NKR-P1B, Ly-55, CD161b, CD161c
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 mouse splenocytes were stained with FITC anti-mouse CD49b and APC/Fire™ 810 anti-mouse NK-1.1 (clone S17016D) (left) or unstained for APC/Fire™ 810 (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
156519 | 25 µg | £94 | ||||
156520 | 100 µg | £254 |
NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Mouse NK1.1-transfectants
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page. - Excitation Laser
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Red Laser (633 nm)
- Application Notes
-
Clone S17016D cross-blocks anti-mouse NK1.1 clone PK136, and can stain for NK1.1 post-formaldehyde and methanol-based fixation based on in-house testing.
- RRID
-
AB_2894654 (BioLegend Cat. No. 156519)
Antigen Details
- Structure
- NKR-P1 gene family
- Distribution
-
NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)
- Function
- NK cell activation, IFN-γ production, cytotoxic granule release
- Cell Type
- NK cells, NKT cells
- Biology Area
- Immunology, Innate Immunity
- Antigen References
-
- Lanier LL. 1997. Immunity 6:371.
- Yokoyama WM, Seaman WE. 1993. Annu. Rev. Immunol. 11:613.
- Koo GC, et al. 1986. J. Immunol. 137:3742.
- Giorda R, Trucco M. 1991. J. Immunol. 147:1701.
- Gene ID
- 17059 View all products for this Gene ID
- UniProt
- View information about NK-1.1 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC ... -
Purified anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC ... -
APC anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b (DX5) PE a... -
FITC anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC ... -
APC/Cyanine7 anti-mouse NK-1.1 Antibody
C57BL/6 mouse splenocytes were stained with CD49b (DX5) PE a... -
PE/Cyanine7 anti-mouse NK-1.1 Antibody
C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC ... -
APC/Fire™ 750 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b (DX5) PE a... -
Alexa Fluor® 700 anti-mouse NK-1.1 Antibody
C57BL/6 mouse splenocytes were stained with CD49b (DX5) PE a... -
PE/Dazzle™ 594 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with CD49b (DX5) PE a... -
APC/Fire™ 810 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with FITC anti-mouse ... -
PerCP/Cyanine5.5 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with FITC anti-mouse ... -
PE/Cyanine5 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with FITC anti-mouse ... -
KIRAVIA Blue 520™ anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with APC anti-mouse C... -
Spark NIR™ 685 anti-mouse NK-1.1 Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse CD49b... -
Spark YG™ 581 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with anti-mouse CD49b... -
PE/Fire™ 700 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with anti-mouse CD49d... -
Spark Red™ 718 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with anti-mouse CD49b... -
PE/Fire™ 810 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with anti-mouse CD49... -
Brilliant Violet 421™ anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with anti-mouse CD49b... -
Brilliant Violet 785™ anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with anti-mouse CD49b... -
Brilliant Violet 711™ anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with anti-mouse CD49b... -
Brilliant Violet 650™ anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with anti-mouse CD49b... -
PE/Fire™ 640 anti-mouse NK-1.1
C57BL/6 mouse splenocytes were stained with anti-mouse CD49b...
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