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Brefeldin A Solution (1,000X)

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Regulatory Status
RUO
Other Names
BFA
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420601 1 mL £45
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Description

Brefeldin A (BFA) is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, BFA leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium.
Concentration
5.0 mg/ml
Storage & Handling
Store the Brefeldin A Solution (1,000X) between 2°C and 8°C. Note: DMSO freezes at this temperature.
Application

ICFC - Quality tested
Recommended Usage

Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability.

Application Notes

Completely thaw the solution before use, DMSO freezes at 4°C.  We recommend aliquotting the reagent into smaller volumes to avoid repeated freeze-thawing.  

Application References

(PubMed link indicates BioLegend citation)
  1. O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
  2. Fuse S, et al. 2007. J. Immunol. 178:5227.
  3. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  4. Redfern CH,et al.2006.J. Clin Oncol.19:3107. PubMed
  5. Smithey MJ, et al. 2008. J. Immunol. 180:3406. PubMed
  6. Elzey BD, et al. 2008. Blood 111:38684. PubMed
  7. Nguyen KD, et al. 2008. J. Immunol. 181:5386. PubMed
  8. Miner MD, et al. 2008. Microb Pathog. 45:273. PubMed
  9. Goodridge HS, et al. 2009. J. Immunol. 182:1146. PubMed
  10. Markey KA, et al. 2009. Blood 113:5644. PubMed
  11. Wilcox RA, et al. 2009. Blood PubMed
  12. Zenaro E, et al. 2009. J. Leukoc Bio. PubMed
  13. Rozkova D, et al. 2009. Clin Immunol. 131:1. PubMed
  14. Butcher JP, et al. 2010. Clin Cancer Res. 16:2065.
  15. Boisvert M, et al. 2010. J. Virol. 84:7782. PubMed
  16. Liu WC, et al. 2010. J. Virol. 84:12011.15. Tawara I, et al. 2011. Clin Cancer Res. 17:77. PubMed
  17. Iwata Y, et al. 2011. Blood. 117:530. PubMed
  18. Schmidt NW, et al. 2011. J. Immunol. 186:3836. PubMed
  19. Langkjaer A, et al. 2012. Clin Immunol. 145:1. PubMed
  20. Molinero LL, et al. 2012. PNAS. 109:18529. PubMed
  21. Li HW, et al. 2012. J. Immunol. 189:5572. PubMed
  22. Wang JM, et al. 2013. J. Virol. 87:4372. PubMed
  23. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  24. Wang Jm, et al. 2013. J Virol. 87:11626. PubMed
  25. Lee TH, et al. 2013. J Biol Chem. 288:32873. PubMed
  26. Shi L, et al. 2014. J. Immunol. 192:649. PubMed
  27. Bulati M, et al. 2014. Exp Gerontol. PubMed
  28. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  29. Tuna H, et al. 2014. J Leukoc Biol. 95:471. PubMed
  30. Miller ML, et al. 2014. PNAS. 111:7397. PubMed
  31. Wang Y, et al. 2014. Sci Rep. 4:5346. PubMed
  32. Zhou GL, et al. 2014. J Cell Sci. 127:3916. PubMed
  33. Rist MJ, et al. 2015. J Virol. 89:703. PubMed
Product Citations
  1. Wang R, et al. 2018. Oncoimmunology. 7:e1461303. PubMed
  2. Sun Y, et al. 2022. STAR Protoc. 3:101479. PubMed
  3. Redelman-Sidi G, et al. 2022. Cancer Immunol Res. 10:1241. PubMed
  4. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  5. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  6. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  7. Yang WL, et al. 2023. Nat Commun. 14:863. PubMed
  8. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  9. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
  10. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  11. Mohammadpour H, et al. 2023. Cell Rep. 42:112250. PubMed
  12. Jung HY, et al. 2023. Oncoimmunology. 12:2190671. PubMed
  13. Li Y, et al. 2023. Vaccines (Basel). 11:. PubMed
  14. Onyshchenko K, et al. 2023. Nat Commun. 14:2087. PubMed
  15. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  16. Ko ES, et al. 2023. Cell Commun Signal. 21:107. PubMed
  17. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  18. Thomas OG, et al. 2023. Sci Adv. 9:eadg3032. PubMed
  19. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
  20. Adamo S, et al. 2023. Cell Host Microbe. 31:928. PubMed
  21. LaPorte KM, et al. 2023. J Immunother Cancer. 11:. PubMed
  22. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  23. Yakkala C, et al. 2021. Front Immunol. 12:695150. PubMed
  24. Zheng B, et al. 2021. Int J Mol Sci. 22: . PubMed
  25. Alexander KL, et al. 2021. Gastroenterology. 161:522. PubMed
  26. Trinks N, et al. 2021. Commun Biol. 4:1151. PubMed
  27. Chen J, et al. 2022. Adv Healthc Mater. 11:e2101714. PubMed
  28. Persaud AT, et al. 2022. J Leukoc Biol. 111:573. PubMed
  29. Yu L, et al. 2022. FEBS J. 289:3241. PubMed
  30. Ritzel RM, et al. 2022. Geroscience. 44:1407. PubMed
  31. D'Angeli V, et al. 2022. Eur J Immunol. 52:1058. PubMed
  32. Lu R, et al. 2022. Scand J Immunol. 96:e13177. PubMed
  33. Kanbar JN, et al. 2022. J Exp Med. 219: . PubMed
  34. Minichino D, et al. 2022. J Leukoc Biol. 112:1089. PubMed
  35. Gao KM, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202327119. PubMed
  36. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  37. Jondle CN, et al. 2022. J Virol. 96:e0063922. PubMed
  38. Blanas A, et al. 2022. iScience. 25:104719. PubMed
  39. Hsieh T, et al. 2022. Front Immunol. 13:901030. PubMed
  40. Li FL, et al. 2022. EMBO J. 41:e110698. PubMed
  41. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  42. Dutt TS, et al. 2022. Cell Rep. 41:111783. PubMed
  43. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  44. Shamriz O, et al. 2023. Front Immunol. 13:1044933. PubMed
  45. Chan L, et al. 2022. Cells. 12: . PubMed
  46. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  47. Nanishi E, et al. 2023. NPJ Vaccines. 8:18. PubMed
  48. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  49. Li Y, et al. 2022. Brain Behav Immun. 101:1. PubMed
  50. Zander R, et al. 2022. Immunity. 55:475. PubMed
  51. Pymm P, et al. 2022. Cell Rep. 38:110449. PubMed
  52. Giles JR, et al. 2022. Immunity. 55:557. PubMed
  53. Ye L, et al. 2022. Cell Metab. 34:595. PubMed
  54. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  55. Zhao Y, et al. 2022. ACS Appl Mater Interfaces. 14:21872. PubMed
  56. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  57. Wang Y, et al. 2014. Sci Rep. 4:5346. PubMed
  58. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  59. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  60. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  61. Zhu S, et al. 2017. Exp Cell Res.. 10.1016/j.yexcr.2017.10.013. PubMed
  62. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  63. Xin G, et al. 2021. Cancer Immunol Res. 9:454. PubMed
  64. Rodrigues M, et al. 2022. Biochem J. 479:1891. PubMed
  65. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
  66. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  67. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  68. Fote GM, et al. 2022. J Cell Sci. 135:. PubMed
  69. Aguilar-Pineda JA, et al. 2021. iScience. 24:102993. PubMed
  70. Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
  71. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  72. Pablo-Bernal R, et al. 2016. J Gerontol A Biol Sci Med Sci. 71: 610 - 618. PubMed
  73. Tuna H, et al. 2014. J Leukoc Biol. 95:471. PubMed
  74. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  75. Yamamoto A, et al. 2019. J Clin Invest. 129:3640. PubMed
  76. Weng RR, et al. 2021. Nat Commun. 12:2163. PubMed
  77. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  78. Shi L, et al. 2014. J Immunol. 192:649. PubMed
  79. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  80. Liang Q, et al. 2022. iScience. 25:104043. PubMed
  81. Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed
  82. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  83. Krone A, et al. 2022. Sci Rep. 218:. PubMed
  84. Michaud D, et al. 2022. Front Immunol. 12:745873. PubMed
  85. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  86. Gay MD, et al. 2020. Cancer Prev Res (Phila). . PubMed
  87. Just S, et al. 2016. Sci Rep. 6:39796. PubMed
  88. Nguyen K, et al. 2008. J Immunol. 181:5386. PubMed
  89. Wilcox R, et al. 2009. Blood. 114:2936. PubMed
  90. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  91. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  92. Starnbach X 2015. J Immunol. 195: 1665-1675. PubMed
  93. Garg B, et al. 2016. Mutagenesis. 10.1093/mutage/gew023. PubMed
  94. Ruhland M, et al. 2016. Nat Commun. 7:11762. PubMed
  95. Shi Z, et al. 2021. Ann Clin Transl Neurol. 8:43. PubMed
  96. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  97. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  98. Smithey M, et al. 2008. J Immunol. 180:3406. PubMed
  99. Rana A, et al. 2020. Front Immunol. 11:536. PubMed
  100. Dayoub O, et al. 2017. Sci Rep. 10.1038/s41598-017-09933-4. PubMed
  101. Kang X, et al. 2022. Vaccines (Basel). 10:. PubMed
  102. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  103. Jondle CN, et al. 2021. MBio. 12:. PubMed
  104. Martens A, et al. 2020. Nat Immunol. 21:381. PubMed
  105. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  106. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  107. Hradilova N, et al. 2017. PLoS One. 12(2):e0171539. PubMed
  108. Griffiths K, et al. 2016. Nat Commun. 7:13894. PubMed
  109. Ciarlo E, et al. 2016. Sci Rep. 6:37944. PubMed
  110. Al-Attar A, et al. 2016. Data Brief. 8: 536-44. PubMed
  111. Gonzalez-Perez G, et al. 2016. J Immunol. 196: 3768 - 3779. PubMed
  112. Pablo-Bernal R, et al. 2015. J Infect Dis. 10.1093/infdis/jiv520. PubMed
  113. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  114. Yu H, et al. 2011. J Leukoc Biol. 90:205. PubMed
  115. Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed
  116. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  117. Doll K, et al. 2016. J Immunol. 196: 4253 - 4262. PubMed
  118. Liu W, et al. 2016. J Virol. 90: 6085 - 6096. PubMed
  119. Fucikova J, et al. 2016. Blood. 128(26):3113-3124. PubMed
  120. Douthwaite J, et al. 2017. J Immunol. 198(1):528-537. PubMed
  121. Liu W, et al. 2010. J Virol. 84:12011. PubMed
  122. Elzey B, et al. 2008. Blood. 111:3684. PubMed
  123. Ries M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006506. PubMed
  124. Rialdi A, et al. 2016. Science. 352: aad7993. PubMed
  125. Li H, et al. 2012. J Immunol. 189:5572. PubMed
  126. Carissimo G, et al. 2019. Life Sci Alliance. 2:e201900298. PubMed
  127. Wang Y, et al. 2020. Sci Adv. 6:eaba5412. PubMed
  128. Littwitz-Salomon E, et al. 2021. Nat Commun. 12:5376. PubMed
  129. Shenoy AT, et al. 2021. Nat Commun. 12:5834. PubMed
  130. Ulrich-Lewis JT, et al. 2022. Front Immunol. 13:861710. PubMed
  131. Elliott PR, et al. 2021. Cell Rep. 37:109777. PubMed
  132. Dumaine JE, et al. 2021. Elife. 10:. PubMed
  133. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  134. Jondle CN, et al. 2021. J Virol. 95:e0072621. PubMed
  135. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  136. Uddin MN, et al. 2022. Brain Behav Immun Health. 26:100563. PubMed
  137. Satoh M, et al. 2016. Sci Rep. 6:28473. PubMed
  138. Codo AC, et al. 2020. Cell Metab. 32:437. PubMed
  139. Tawara I, et al. 2011. Clin Cancer Res. 0.761805556. PubMed
  140. Orlov M, et al. 2017. PLoS One.. 10.1371/journal.pone.018296. PubMed
  141. Martins M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006529. PubMed
  142. Crooks SD, et al. 2022. Immunohorizons. 6:705. PubMed
  143. Fang E, et al. 2022. MedComm (2020). 3:e117. PubMed
  144. Gross C, et al. 2016. Neurol Neuroimmunol Neuroinflamm. 3: e289. PubMed
  145. Krämer B, et al. 2016. PLoS One. 11: 0162068. PubMed
  146. Cao Y, et al. 2018. Nat Commun. 2.940972222. PubMed
  147. Emmer BT, et al. 2018. Elife. 7:e38839. PubMed
  148. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  149. Harty N 2011. J Immunol. 186:3836. PubMed
  150. Iwata Y, et al. 2011. Blood. 117:530. PubMed
  151. Lee T, et al. 2013. J Biol Chem. 288:32873. PubMed
  152. Vigano S, et al. 2015. J Virol. 89: 7829-7840. PubMed
  153. Vacaflores A, et al. 2016. PLoS One. 11: 0157175. PubMed
  154. Ma H, Wan S, Xia C 2016. J Leukoc Biol. 99: 1121 - 1129. PubMed
  155. Kim J, et al. 2016. Nat Immunol. 17:1159-1166. PubMed
  156. Johnson AM, et al. 2020. J Immunol. 204:2295. PubMed
  157. Buqué A, et al. 2020. Nat Commun. 3.110416667. PubMed
  158. Redfern C, et al. 2006. J Clin Oncol. 24:3107. PubMed
  159. Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed
  160. Li G, et al. 2021. Curr Protoc. 1:e50. PubMed
  161. Gao Y, et al. 2022. Immunity. 55:1732. PubMed
  162. Han K, et al. 2021. Nat Metab. 3:318. PubMed
  163. Peng L, et al. 2022. Cell Rep Med. 3:100634. PubMed
  164. Bachy S, et al. 2022. iScience. 25:103758. PubMed
  165. Ohtsuka J, et al. 2021. iScience. Online ahead of print. PubMed
  166. Yan H, et al. 2020. ACS Appl Mater Interfaces. 12:19324. PubMed
  167. Matisz C, et al. 2017. Sci Rep. 7:40631. PubMed
  168. Castro I, et al. 2016. J Virol. 90: 5280 - 5291. PubMed
  169. Zhang W, et al. 2015. Infect Immun . 83: 3213-3223. PubMed
  170. Ji L, et al. 2012. PLoS One. 7:e50909. PubMed
  171. Ngwenyama N, et al. 2019. JCI Insight. 4:e125527. PubMed
  172. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  173. Butchar J, et al. 2010. Clin Cancer Res. 2.100694444. PubMed
  174. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  175. Grace PS, et al. 2021. Front Immunol. 12:679973. PubMed
  176. Li Y, et al. 2021. Vaccines (Basel). 9:. PubMed
  177. Liu W, et al. 2022. Bio Protoc. 12:e4285. PubMed
  178. Ma K, et al. 2022. iScience. 25:104347. PubMed
  179. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  180. Wang J, et al. 2013. J Virol. 87:4372. PubMed
  181. Nice T, et al. 2016. PLoS One. 12: 1005684. PubMed
  182. Pfeifle R, et al. 2016. Nat Immunol. 18:104-113. PubMed
  183. Krishnan A, et al. 2020. Am J Pathol. 190:1284. PubMed
  184. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  185. Sekine T, et al. 2020. Cell. 183:158. PubMed
  186. Gogokhia L, et al. 2019. Cell Host Microbe. 25:285. PubMed
  187. Guo C, et al. 2018. Immunity. 49:842. PubMed
  188. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  189. Rossato C, et al. 2017. Biologicals. 10.1016/j.biologicals.2017.06.007. PubMed
  190. Tajima M, et al. 2022. Curr Protoc. 2:e540. PubMed
  191. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  192. Michelozzi IM, et al. 2022. STAR Protoc. 3:101174. PubMed
  193. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  194. Stepanova E, et al. 2021. Microorganisms. 9:. PubMed
  195. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  196. Loeuillard E, et al. 2020. J Clin Invest. 130:5380. PubMed
  197. Matsushita T, et al. 2015. Rheumatology. 101093/rumatology/kv331. PubMed
  198. Molinero L, et al. 2012. Proc Natl Acad Sci U S A. 109:18529. PubMed
  199. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  200. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  201. Zenaro E, et al. 2009. J Leukoc Biol. 86:1393. PubMed
  202. Goodridge H, et al. 2009. J Immunol. 182:1146. PubMed
  203. Gnerlich J, et al. 2010. J Immunol. 185:4063. PubMed
  204. Boisvert M, et al. 2010. J Virol. 84:7782. PubMed
  205. Tse SW, et al. 2021. Mol Ther. 29:2227. PubMed
  206. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  207. Langkjær A, et al. 2012. Clin Immunol. 145:1. PubMed
  208. Rozková D, et al. 2009. Clin Immunol. 131:1. PubMed
  209. Miner M, et al. 2008. Microb Pathog. 45:273. PubMed
  210. Miller M, et al. 2014. Proc Natl Acad Sci U S A. 111:7397. PubMed
  211. Gartlan K, et al. 2015. Blood. 126: 1609-1620. PubMed
  212. Ulland T, et al. 2016. Nat Commun. 7:13180. PubMed
  213. Melacarne A, et al. 2021. Cell Reports. 36(1):109312. PubMed
  214. Markey K, et al. 2009. Blood. 113:5644. PubMed
  215. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  216. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  217. Ma Q, et al. 2022. Vaccines (Basel). 10:. PubMed
  218. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  219. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  220. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  221. Li Z, et al. 2021. JCI Insight. 6:. PubMed
  222. Virtakoivu R, et al. 2021. Clin Cancer Res. 27:4205. PubMed
  223. Koschel J, et al. 2021. Cell Death Differ. 28:2257. PubMed
  224. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  225. Bass VL, et al. 2021. Mol Syst Biol. 17:e10127. PubMed
  226. Theisen U, et al. 2020. J Cell Biol. :219. PubMed
  227. Dáňová K, et al. 2017. J Immunol. 198(2):729-740. PubMed
  228. Beyaz S, et al. 2016. Nat Immunol. 18:184-195. PubMed
  229. Kadivar M, et al. 2016. J Immunol. 197(12):4584-4592. PubMed
  230. Wang J, et al. 2013. J Virol. 87:11262. PubMed
  231. Landi A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.072. PubMed
  232. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  233. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  234. Bulati M, et al. 2014. Exp Gerontol. 54:123. PubMed
  235. Zhou G, et al. 2014. J Cell Sci. 127:3916. PubMed
  236. Rist M, et al. 2015. J Virol. 89:703. PubMed
  237. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  238. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  239. Mohammadpour H, et al. 2020. JCI Insight. 5:00. PubMed
  240. Gebremeskel S, et al. 2017. Cancer Immunol Res. 0.9625. PubMed
  241. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  242. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  243. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  244. Genardi S, et al. 2020. Front Immunol. 11:610010. PubMed
  245. Wang F, et al. 2021. Neoplasia. 23:281. PubMed
  246. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  247. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  248. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  249. Rau M, et al. 2016. J Immunol. 196: 97 - 105. PubMed
  250. Ruland C, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.06.010. PubMed
  251. Ahmadi H,et al. 2017. Inflammopharmacology. . 10.1007/s10787-017-0410-8. PubMed
  252. Li J, et al. 2018. Redox Biol. 0.976388889. PubMed
  253. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  254. Krishna B, et al. 2016. Sci Rep. 6: 24674. PubMed
  255. Schridde A, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.142. PubMed
  256. Wang W, et al. 2020. STAR Protoc. 1:100218. PubMed
  257. Lu G, et al. 2017. PLoS One. 12:e0178352. PubMed
  258. Orozco SL, et al. 2019. Cell Rep. 28:2275. PubMed
  259. Ying B, et al. 2022. Sci Transl Med. 14:eabm3302. PubMed
  260. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  261. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  262. Petrova T, et al. 2021. FEBS J. 288:5909. PubMed
  263. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  264. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  265. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  266. Abshire C, et al. 2016. PLoS Pathog. 12:e1006088. PubMed
  267. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
5. Jung T, et al. 1993. J. Immunol. Meth. 159:197.

Gene ID
NA

Related FAQs

The Brefeldin A solution freezes at 4°C . Is the solution still OK?

We recommend to thaw in warm water after removing from the fridge.  The Brefeldin A is dissolved in DMSO, which freezes at 18°C, so this is normal.  No one has reported any problems with the freeze/thaw cycles and this is how we recommend it is stored.   If customers are concerned, they can aliquot the Brefeldin A to avoid the freeze thaw.

How do I choose monensin or Brefeldin A solution?

Generally, Brefeldin A is more toxic for longer term incubations, so for shorter stimulations (6 hrs or less) use Brefeldin A.  For longer stimulations use monensin.  We recommend optimization for each cell type and protocol.

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For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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