- Clone
- NP4D6 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HLDA8
- Other Names
- E-NPP3, ENPP3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![NP4D6_BV421_1_051311 NP4D6_BV421_1_051311](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/NP4D6_BV421_1_051311.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD193 APC and CD203c (clone NP4D6) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom). -
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
324611 | 25 tests | £140 | ||||
324612 | 100 tests | £298 |
CD203c, a transmembrane protein and a member of the ectoenzyme family, is involved in the hydrolysis of extracellular oligonucleotides, nucleoside phosphates, and NAD (possesses ATPase and ATP pyrophosphatase activity). The molecular weight of CD203c is between 130 and 150 kD under reducing conditions and 270 kD under non-reducing conditions. CD203c is expressed on basophils and mast cells, and is highly expressed on activated basophils. Secretory glands in endometrium and glioma cells are also positive. CD203c is a multifunctional ectoenzyme involved in the clearance of extracellular nucleotides whose substrates include nucleoside triphosphates, nucleoside diphosphates, cAMP, and NAD.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- HEK-293 cells transfected with human E-NPP3
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_10900984 (BioLegend Cat. No. 324611)
AB_2563848 (BioLegend Cat. No. 324612)
Antigen Details
- Structure
- Transmembrane ectoenzyme family member involved in the hydrolysis of extracellular oligonucleotides, nucleoside phosphates, and NAD (possesses ATPase and ATP pyrophosphatase activity); reduced molecular weight is 130 and 150 kD, unreduced is 270 kD
- Distribution
-
Basophils and mast cells, highly expressed on activated basophils; secretory glands in endometrium and glioma cells are also positive
- Function
- Multifunctional ectoenzyme involved in the clearance of extracellular nucleotides
- Ligand/Receptor
- Substrates include nucleoside triphosphates, nucleoside diphosphates, cAMP, and NAD
- Cell Type
- Basophils, Mast cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Yano Y, et al. 2003. Int. J. Mol. Med. 12:763.
2. Andoh K, et al. 1999. Biochim. Biophys. Acta. 1446:213. - Gene ID
- 5169 View all products for this Gene ID
- UniProt
- View information about CD203c on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD203c Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD203c (E-NPP3) | NP4D6 | FC |
PE anti-human CD203c (E-NPP3) | NP4D6 | FC |
Brilliant Violet 510™ anti-human CD203c (E-NPP3) | NP4D6 | FC |
Brilliant Violet 605™ anti-human CD203c (E-NPP3) | NP4D6 | FC |
APC anti-human CD203c (E-NPP3) | NP4D6 | FC |
PerCP/Cyanine5.5 anti-human CD203c (E-NPP3) | NP4D6 | FC |
Brilliant Violet 421™ anti-human CD203c (E-NPP3) | NP4D6 | FC |
FITC anti-human CD203c (E-NPP3) | NP4D6 | FC |
PerCP anti-human CD203c (E-NPP3) | NP4D6 | FC |
PE/Cyanine7 anti-human CD203c (E-NPP3) | NP4D6 | FC |
PE/Dazzle™ 594 anti-human CD203c (E-NPP3) | NP4D6 | FC |
Alexa Fluor® 647 anti-human CD203c (E-NPP3) | NP4D6 | FC |
TotalSeq™-A0593 anti-human CD203c (E-NPP3) | NP4D6 | PG |
TotalSeq™-C0593 anti-human CD203c (E-NPP3) | NP4D6 | PG |
TotalSeq™-D0593 anti-human CD203c (E-NPP3) | NP4D6 | PG |
TotalSeq™-B0593 anti-human CD203c (E-NPP3) | NP4D6 | PG |
PE/Cyanine7 anti-human CD203c | NP4D6 | FC |
PE anti-human CD203c | NP4D6 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD203c (E-NPP3)
Human basophilic leukemia cell line KU-812 stained with puri... -
PE anti-human CD203c (E-NPP3)
Human basophilic leukemia cell line KU-812 stained with NP4D... -
Brilliant Violet 510™ anti-human CD203c (E-NPP3)
Overnight cultured human peripheral blood mononuclear cells ... -
Brilliant Violet 605™ anti-human CD203c (E-NPP3)
Overnight cultured human peripheral blood lymphocytes were s... -
APC anti-human CD203c (E-NPP3)
Human peripheral blood lymphocytes were stained with CD193 P... -
PerCP/Cyanine5.5 anti-human CD203c (E-NPP3)
Human peripheral blood lymphocytes were stained with CD193 (... -
Brilliant Violet 421™ anti-human CD203c (E-NPP3)
Human peripheral blood lymphocytes were stained with CD193 A... -
FITC anti-human CD203c (E-NPP3)
Overnight cultured human peripheral blood mononuclear cells ... -
PerCP anti-human CD203c (E-NPP3)
Overnight cultured human peripheral blood mononuclear cells ... -
PE/Cyanine7 anti-human CD203c (E-NPP3)
Overnight cultured human peripheral blood lymphocytes were s... -
PE/Dazzle™ 594 anti-human CD203c (E-NPP3)
Human peripheral blood lymphocytes were stained with CD193 A... -
Alexa Fluor® 647 anti-human CD203c (E-NPP3)
Human peripheral blood lymphocytes were stained with CD193 P... -
TotalSeq™-A0593 anti-human CD203c (E-NPP3)
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TotalSeq™-C0593 anti-human CD203c (E-NPP3)
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TotalSeq™-D0593 anti-human CD203c (E-NPP3)
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TotalSeq™-B0593 anti-human CD203c (E-NPP3)
-
PE/Cyanine7 anti-human CD203c
Typical results from Human peripheral blood lymphocytes stai... -
PE anti-human CD203c
Typical results from human peripheral blood lymphocytes stai...
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