- Clone
- S15049I (See other available formats)
- Regulatory Status
- RUO
- Other Names
- M130, MM130, SCARI1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![S15049I_BV421_CD163_Antibody_080719 S15049I_BV421_CD163_Antibody_080719](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S15049I_BV421_CD163_Antibody_080719.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 bone marrow were stained with CD11b APC and CD163 Brilliant Violet 421™ (clone S15049I, left), or rat IgG2a, κ Brilliant Violet 421™ isotype control (right). Data shown was gated on total bone marrow cells.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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155309 | 50 µg | £196 |
CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD163 extracellular domain
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Product Citations
-
- RRID
-
AB_2814063 (BioLegend Cat. No. 155309)
Antigen Details
- Structure
- Type 1 transmembrane glycoprotein, 134 kD (non-reduced)/155 kD (reduced) glycoprotein
- Distribution
-
Macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells
- Function
- Binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- 93671 View all products for this Gene ID
- UniProt
- View information about CD163 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD163 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD163 | S15049I | FC,IHC-F |
Brilliant Violet 421™ anti-mouse CD163 | S15049I | FC |
PE anti-mouse CD163 | S15049I | FC |
APC anti-mouse CD163 | S15049I | FC |
TotalSeq™-A0417 anti-mouse CD163 | S15049I | PG |
TotalSeq™-B0417 anti-mouse CD163 | S15049I | PG |
TotalSeq™-C0417 anti-mouse CD163 | S15049I | PG |
PE/Dazzle™ 594 anti-mouse CD163 Antibody | S15049I | FC |
KIRAVIA Blue 520™ anti-mouse CD163 Antibody | S15049I | FC |
PE/Cyanine7 anti-mouse CD163 Antibody | S15049I | FC |
APC/Fire™ 810 anti-mouse CD163 Antibody | S15049I | FC |
APC/Cyanine7 anti-mouse CD163 | S15049I | FC |
Brilliant Violet 711™ anti-mouse CD163 | S15049I | FC |
Brilliant Violet 605™ anti-mouse CD163 | S15049I | FC |
Alexa Fluor® 647 anti-mouse CD163 | S15049I | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD163
C57BL/6 mouse peritoneal macrophages were cultured for seven... C57BL/6 bone marrow were stained with CD11b APC and purified... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... C57BL/6 mouse frozen liver section was fixed with 4% parafor... -
Brilliant Violet 421™ anti-mouse CD163
C57BL/6 bone marrow were stained with CD11b APC and CD163 Br... -
PE anti-mouse CD163
C57BL/6 bone marrow were stained with CD11b APC and CD163 (c... -
APC anti-mouse CD163
C57BL/6 bone marrow cells were stained with CD11b PE and CD1... -
TotalSeq™-A0417 anti-mouse CD163
-
TotalSeq™-B0417 anti-mouse CD163
-
TotalSeq™-C0417 anti-mouse CD163
-
PE/Dazzle™ 594 anti-mouse CD163 Antibody
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
KIRAVIA Blue 520™ anti-mouse CD163 Antibody
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
PE/Cyanine7 anti-mouse CD163 Antibody
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
APC/Fire™ 810 anti-mouse CD163 Antibody
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
APC/Cyanine7 anti-mouse CD163
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Brilliant Violet 711™ anti-mouse CD163
C57BL/6 mouse bone marrow were stained with anti-mouse CD11b... -
Brilliant Violet 605™ anti-mouse CD163
C57BL/6 bone marrow cells were stained with anti-mouse CD11b... -
Alexa Fluor® 647 anti-mouse CD163
C57BL/6 bone marrow cells were stained with anti-mouse CD11b...
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