LEGEND MAX™ Human α-Synuclein (Colorimetric) ELISA Kit

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Regulatory Status
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Product Citations
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Human_alpha-Synuclein_ELISA_102021
  • Human_alpha-Synuclein_ELISA_102021
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448607 1 Pre-coated Plate £407
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Description

α-Synuclein is a 140-amino-acids long neuronal protein that belongs to the Synuclein family, which also includes β-Synuclein and γ-Synuclein. At the cellular level, α-Synuclein primarily localizes in the synaptic terminals of neurons. However, it can also be found in other cell types such as red blood cells. Although the normal function of α-Synuclein is not fully understood, it was reported to have an important role in synaptic vesicle transport and membrane fusion. Moreover, the aggregate form of α-Synuclein is one of the major components of the inclusion bodies (Lewy bodies) inside nerve cells affected by the Parkinson’s disease and Lewy body dementias.

The LEGEND MAX™ Human α-Synuclein (Colorimetric) ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with anti-human α-Synuclein monoclonal antibody. The detection antibody is a biotinylated anti-human α-Synuclein monoclonal antibody. This kit is specifically designed for the accurate quantitation of human α-Synuclein from cerebrospinal fluid, serum, plasma, urine, tissue lysate, and cell culture sup. It is analytically validated with ready-to-use reagents. 

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human α-Synuclein pre-coated 96-well Strip Microplate
  • Human α-Synuclein Detection Antibody
  • Human α-Synuclein Lyophilized Standard
  • Avidin HRP
  • 2X Reagent Diluent
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA

Product Citations
  1. Wu S, et al. 2023. Elife. 12:. PubMed
Sensitivity
1.80 ± 0.21 pg/mL
Standard Range
10.2 - 650 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Type
Neural Stem Cells, Neurons
Biology Area
Cell Death, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation
Molecular Family
Synaptic Vesicle Trafficking/Endocytosis
Gene ID
NA
UniProt
View information about alpha-Synuclein on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 10/20/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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