MojoSort™ Mouse CD8a Selection Kit

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MojoSort_Mouse_CD8a_Positive_Selection_Kit_102419.png
A single cell suspension from C57BL/6 mouse spleen was prepared for the positive selection of CD8a+ cells using the MojoSort™ Mouse CD8a Selection Kit. Cells were stained with anti-mouse CD8b (clone YTS156.7.7) PE and anti-mouse CD3 (clone 17A2) APC. Dead cells were excluded by Helix NP™ Blue. Left plot: CD8a+ cells after isolation. Right plot: Cells before isolation.
  • MojoSort_Mouse_CD8a_Positive_Selection_Kit_102419.png
    A single cell suspension from C57BL/6 mouse spleen was prepared for the positive selection of CD8a+ cells using the MojoSort™ Mouse CD8a Selection Kit. Cells were stained with anti-mouse CD8b (clone YTS156.7.7) PE and anti-mouse CD3 (clone 17A2) APC. Dead cells were excluded by Helix NP™ Blue. Left plot: CD8a+ cells after isolation. Right plot: Cells before isolation.
Cat # Size Price Quantity Check Availability Save
480135 20 tests £141
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480136 200 tests £484
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Description

Mouse CD8a+ cells are either selected or depleted by incubating the sample with a biotin conjugated anti-mouse CD8a antibody and then magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD8a+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480135:

  • 1 vial containing 100 µL of Biotin anti-mouse CD8a antibody (100X) (clone 53-6.7)
  • 1 vial containing 200 µL of Streptavidin Nanobeads

For Cat# 480136:

  • 1 vial containing 1 mL of Biotin anti-mouse CD8a antibody (100x) (clone 53-6.7)
  • 2 vials containing 1 mL each of Streptavidin Nanobeads

Product Details

Verified Reactivity
Mouse
Formulation
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2 with stabilizer.

Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.Streptavidin Nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µL of pre-diluted Biotin anti-mouse CD8a antibody for 1 x 107 cells in 100 µL of buffer. Note: Prepare the pre-diluted antibody by adding 5 µL of the Biotin anti-mouse CD8a (100X) antibody to 495 µL 1X MojoSort Buffer*.

*Suggested 100X dilution volumes results in excess diluted antibody per test. We discourage performing a 100X dilution using smaller volumes of stock antibody (e.g. 1 µL antibody + 99 µL Buffer) due to increased likelihood for pipetting error and affecting cell separation results.

10 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer

Application Notes

This kit is designed for the positive selection or depletion of mouse CD8a+ cells from mouse splenocytes.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.

Antibody or cocktail dilution to use in column: 200X
Nanobead dilution to use in columns: 4X

Product Citations
  1. Romine KA, et al. 2023. Leukemia. 37:580. PubMed
  2. Wang YM, et al. 2022. Oncoimmunology. 11:2034257. PubMed

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.

 

MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.

What is the size of your magnetic particles?

MojoSort Nanobeads: the average diameter is approximately 130 nm.

 

MojoSort Microbeads: the average diameter is approximately 1 μm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 2    Revision Date: 05/25/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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