- Clone
- BC96 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V T-072
- Other Names
- Low affinity IL-2R, IL-2R α chain, Tac, p55
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![BC96_Blue_071108.jpg BC96_Blue_071108.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/BC96_Blue_071108.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
302620 | 100 µg | £182 | ||||
302627 | 100 tests | £193 |
CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
-
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. - Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per 106 cells in 100 µl volume.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤1.0 µg per 106 cells in 100 µl volume.
It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
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Additional reported applications include: immunocytochemistry3.
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
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AB_493649 (BioLegend Cat. No. 302620)
AB_2233706 (BioLegend Cat. No. 302627)
Antigen Details
- Structure
- Type I transmembrane glycoprotein, 55 kD
- Distribution
-
Activated T cells and B cells, monocytes/macrophages, Treg
- Function
- Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- B cells, Macrophages, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
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1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681. - Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Other Formats
View All CD25 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
FITC anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
PE anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
PE/Cyanine5 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
Purified anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
APC/Cyanine7 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
PE/Cyanine7 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
Alexa Fluor® 488 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
Alexa Fluor® 647 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
Pacific Blue™ anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
Alexa Fluor® 700 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
Biotin anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
PerCP/Cyanine5.5 anti-human CD25
PHA-stimulated (day 3) human peripheral blood lymphocytes we... -
Brilliant Violet 421™ anti-human CD25
Human peripheral blood lymphocytes were stained with CD4 FIT... -
Brilliant Violet 605™ anti-human CD25
Human peripheral blood lymphocytes were stained with CD4 FIT... -
Brilliant Violet 650™ anti-human CD25
Human peripheral blood lymphocytes were stained with CD4 FIT... -
Brilliant Violet 711™ anti-human CD25
Human peripheral blood lymphocytes were stained with CD4 FIT... -
Brilliant Violet 785™ anti-human CD25
Human peripheral blood lymphocytes were stained with CD4 FIT... -
Brilliant Violet 510™ anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
APC/Fire™ 750 anti-human CD25
PHA-stimulated (day 4) human peripheral blood lymphocytes we... -
TotalSeq™-A0085 anti-human CD25
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PE/Dazzle™ 594 anti-human CD25
PHA-stimulated (3 day) human peripheral blood lymphocytes we... -
TotalSeq™-B0085 anti-human CD25
-
TotalSeq™-C0085 anti-human CD25
-
TotalSeq™-D0085 anti-human CD25
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