- Clone
- A20120F (See other available formats)
- Regulatory Status
- RUO
- Other Names
- For CD209: CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1, For CD299: LSIGN, CD209L, L-SIGN, DCSIGNR, HP10347, DC-SIGN2, DC-SIGNR
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A20120F_PE_-CD209-CD299_FC_052623 A20120F_PE_-CD209-CD299_FC_052623](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A20120F_PE_-CD209-CD299_FC_052623-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human monocyte-derived dendritic cells were stained with anti-human CD209/CD299 (DC-SIGN/L-SIGN) (clone A20120F) PE (left) or rat IgG2a, κ PE isotype control (right) and costained with anti-human CD11c APC.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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380503 | 25 tests | £89 | ||||
380504 | 100 tests | £230 |
CD209 (DC-SIGN) is a C-type lectin that has a high affinity for the ICAM3 molecule. It binds various microorganisms by recognizing high mannose-containing glycoproteins on their envelopes. It especially functions as a receptor for several viruses such as HIV and Hepatitis C. CD299 (L-SIGN) is a transmembrane receptor and is referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. The protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain, and a C-type lectin carbohydrate recognition domain.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human CD209 protein extracellular domain
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone A20120F can completely block the binding of clone 14E3G7 to target cells. It does not affect the binding of clone A20120B, clone 9E9A8 and clone DCS-8C1.
- RRID
-
AB_3068055 (BioLegend Cat. No. 380503)
AB_3068055 (BioLegend Cat. No. 380504)
Antigen Details
- Structure
- DC-SIGN is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. DC-SIGNR (DC-SIGN-related molecule) is a type II integral membrane protein that is 77% identical to DC-SIGN.
- Distribution
-
Dendritic cells, endothelial cells, macrophages
- Function
- CD299 mediates interactions between dendritic cells and resting T cells and has a high affinity for HIV-1 gp120. CD209 plays a critical role in capturing and internalizing pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates the transport of HIV to the proteasome.
- Ligand/Receptor
- CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTNA2A1). CD299 is a receptor for ICAM-3 (CD50).
- Cell Type
- Dendritic cells, Endothelial cells, Macrophages
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
- Fauci AS. 1996. Nature. 384:529-34.
- Yokoyama-Kobayashi M, et al. 1999. Gene. 228:161-7.
- Soilleux EJ, et al. 2000. J Immunol. 165:2937-42.
- Pohlmann S, et al. 2001. Proc. Natl. Acad. Sci. USA 98:2670-5.
- Bashirova AA, et al. 2001. J Exp Med. 193:671-8.
- Lai WK, et al. 2006. Am J Pathol. 169:20-8.
- Gene ID
- 30835 View all products for this Gene ID 10332 View all products for this Gene ID
- UniProt
- View information about CD209/CD299 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD209/CD299 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD209/CD299 (DC-SIGN/L-SIGN) | A20120F | FC |
PE anti-human CD209/CD299 (DC-SIGN/L-SIGN) | A20120F | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD209/CD299 (DC-SIGN/L-SIGN)
Human monocyte-derived dendritic cells were stained with pur... -
PE anti-human CD209/CD299 (DC-SIGN/L-SIGN)
Human monocyte-derived dendritic cells were stained with ant...
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