- Clone
- 16B5C71 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IK1, IKZF1
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![16B5C71_PECyanine7_Ikaros_Antibody_1_102423 16B5C71_PECyanine7_Ikaros_Antibody_1_102423](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/16B5C71_PECyanine7_Ikaros_Antibody_1_102423.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood mononuclear cells were stained with CD19 PE/Dazzle™ 594, treated with the True-Nuclear™ Transcription Factor Buffer Set, and then stained with Ikaros (clone 16B5C71) PE/Cyanine7 (left) or Mouse IgG2a, κ PE/Cyanine7 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
368413 | 25 tests | £113 | ||||
368414 | 100 tests | £282 |
Ikaros, also known as IKZF1, belongs to a family of zinc-finger DNA-binding proteins that regulate lymphocyte differentiation. It has the ability to form homo- and heterodimers with other members of the Ikaros family, which include Aiolos and Helios. Ikaros can be found on a majority of fetal and adult hematopoietic cell types such as T- and B-cells, natural killer cells (NKs), monocytes/macrophages, and dendritic cells. Ikaros is a tumor suppressor and its absence or reduced expression has been reported in several leukemias.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
There are eight splicing variants with predicted MW 58, 53, 48(2), 43, 41, 31, 24 KDa. This antibody might recognize some of the isoforms.
- Additional Product Notes
-
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- RRID
-
AB_2734415 (BioLegend Cat. No. 368413)
AB_2734416 (BioLegend Cat. No. 368414)
Antigen Details
- Structure
- Zinc-finger DNA binding protein, homo/heterodimer, 57.5 kD
- Distribution
-
Fetal and adult hematopoietic cells.
- Function
- Regulates lymphocyte differentiation.
- Interaction
- DNA.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Wargnier A, et al. 1995. Proc. Natl. Acad. Sci. USA 92:6930.
2. Tonnelle C, et al. 2009. Mol. Immunol. 46:1736.
3. Wang H, et al. 2014. Pediatr. Blood Cancer 61:2230. - Gene ID
- 10320 View all products for this Gene ID
- UniProt
- View information about Ikaros on UniProt.org
Other Formats
View All Ikaros Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human Ikaros | 16B5C71 | WB,ChIP |
Alexa Fluor® 647 anti-human Ikaros | 16B5C71 | ICFC |
Brilliant Violet 421™ anti-human Ikaros | 16B5C71 | ICFC |
Alexa Fluor® 488 anti-human Ikaros | 16B5C71 | ICFC |
PE anti-human Ikaros | 16B5C71 | ICFC |
APC anti-human Ikaros | 16B5C71 | ICFC |
PE/Cyanine7 anti-human Ikaros | 16B5C71 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Ikaros
Total lysates (15 µg protein) from Jurkat (lane 1) and HeLa ... Chromatin Immunoprecipitations (ChIP) were done with fixed a... Chromatin Immunoprecipitations (ChIP) were done with fixed a... -
Alexa Fluor® 647 anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
Brilliant Violet 421™ anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
Alexa Fluor® 488 anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
PE anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
APC anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C... -
PE/Cyanine7 anti-human Ikaros
Human peripheral blood mononuclear cells were stained with C...
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