- Clone
- S18025A (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_S18025A_PECyanine7_CD289_Antibody_052424 A_S18025A_PECyanine7_CD289_Antibody_052424](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/S18025A_PECyanine7_CD289_Antibody_052424.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were surface stained with anti-mouse CD45R/B220 (clone RA3-6B2) FITC. Cells were then fixed, permeabilized, and intracellularly stained with anti-mouse CD289 (TLR9) (clone S18025A) PE/Cyanine7 (left) or rat IgG1, κ PE/Cyanine7 isotype control (right). -
C57BL/6 mouse splenocytes were surface stained with anti-mouse CD317 (BST2, PDCA-1) (clone 927) PE. Cells were then fixed, permeabilized, and intracellularly stained with anti-mouse CD289 (TLR9) (clone S18025A) PE/Cyanine7 (left) or rat IgG1, κ PE/Cyanine7 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
159109 | 25 µg | £121 | ||||
159110 | 100 µg | £262 |
TLR9 is a member of the toll-like receptor family that aids in recognition of pathogen associated molecular patterns (PAMPs). TLR9 recognizes unmethylated CpG sequences in DNA (ie. intracellular bacteria and DNA viruses). TLR9 signals through the MyD88 pathway leading to inflammatory cytokine production, especially production of type I interferons by pDCs.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Proprietary
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Antigen Details
- Distribution
-
B cells, Dendritic cells, pDCs
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Bacterial proteins and Toxins, Immuno-Oncology, Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Innate Immune Signaling, Toll Like Receptors
- Antigen References
-
- Onji M, et al. 2013. Nat. Commun. DOI: 10.1038/ncomms2949
- Krug A, et al. 2001. Eur J Immunol. 10:3026-37.
- Gene ID
- 81897 View all products for this Gene ID
- UniProt
- View information about CD289 on UniProt.org
Other Formats
View All CD289 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD289 (TLR9) | S18025A | ICFC |
PE anti-mouse CD289 (TLR9) | S18025A | ICFC |
FITC anti-mouse CD289 (TLR9) | S18025A | ICFC |
APC anti-mouse CD289 (TLR9) | S18025A | ICFC |
PE/Cyanine7 anti-mouse CD289 (TLR9) | S18025A | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD289 (TLR9)
C57BL/6 mouse splenocytes were stained with CD317 Pacific Bl... -
PE anti-mouse CD289 (TLR9)
C57BL/6 mouse splenocytes were stained with CD317 Pacific Bl... C57BL/6 mouse splenocytes were stained with CD45R/B220 APC/C... -
FITC anti-mouse CD289 (TLR9)
C57BL/6 mouse splenocytes were stained with anti-mouse CD317... C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
APC anti-mouse CD289 (TLR9)
C57BL/6 mouse splenocytes were stained with anti-mouse CD317... C57BL/6 mouse splenocytes were stained with anti-mouse CD45R... -
PE/Cyanine7 anti-mouse CD289 (TLR9)
C57BL/6 mouse splenocytes were surface stained with anti-mou... C57BL/6 mouse splenocytes were surface stained with anti-mou...
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